Cell Biology

CRB3 navigates Rab11 trafficking vesicles to promote γTuRC assembly during ciliogenesis

1. Bo Wang
2. Zheyong Liang
3. Tan Tan
4. Miao Zhang
5. Yina Jiang
6. Yangyang Shang
7. Xiaoqian Gao
8. Shaoran Song
9. Ruiqi Wang
10. He Chen
11. Jie Liu
12. Juan Li
13. Yu Ren
14. Peijun Liu

• Curated by eLife

  eLife assessment

  This is a useful study for scientists interested in cell polarity, epithelial morphogenesis, cancer, and primary cilia. The authors investigate the role of CRB3 in regulating these processes by using a combination of a mammary epithelial cell-specific conditional Crb3 knockout mouse model, and cellular, molecular and biochemical approaches. The results, which are solid, supporting and extending previous findings, suggest that CRB3 affects ciliogenesis by a mechanism involving Rab11 and gamma-TuRC.

Reviewed by eLife

ESYT1 tethers the endoplasmic reticulum to mitochondria and is required for mitochondrial lipid and calcium homeostasis

1. Alexandre Janer
2. Jordan L. Morris
3. Michiel Krols
4. Hana Antonicka
5. Mari J. Aaltonen
6. Zhen-Yuan Lin
7. Anne-Claude Gingras
8. Julien Prudent
9. Eric A. Shoubridge

Reviewed by Review Commons

PerTurboID, a targeted in situ method reveals the impact of kinase deletion on its local protein environment in the cytoadhesion complex of malaria-causing parasites

1. Heledd Davies
2. Hugo Belda
3. Malgorzata Broncel
4. Jill Dalimot
5. Moritz Treeck

• Curated by eLife

  eLife assessment

  This important study combines conditional mutagenesis with proximity labeling to evaluate alterations in a sub-cellular proteome upon a perturbing event. The approach is applied to the deletion of a kinase involved in trafficking of adhesins to the malaria parasite-infected erythrocyte surface and the evidence supporting the conclusions is compelling. The work will be of broad interest to cell biologists and biochemists.

Reviewed by eLife

Aurora A mediated new phosphorylation of RAD51 is observed in Nuclear Speckles

1. Mohamad Alaouid
2. Parfait Kenfack Ymbe
3. Vanessa Philippot-Ménil
4. Gwennina Cueff
5. Alexandre Demeyer
6. Damien Marquis
7. Nizar Ayadi
8. Fabrice Fleury
9. Houda Benhelli-Mokrani

Reviewed by Review Commons

The actin binding protein profilin 1 localizes inside mitochondria and is critical for their function

1. Tracy-Ann Read
2. Bruno A Cisterna
3. Kristen Skruber
4. Samah Ahmadieh
5. Tatiana M Liu
6. Josefine A Vitriol
7. Yang Shi
8. Joseph B Black
9. Mitchell T Butler
10. Halli L Lindamood
11. Austin EYT Lefebvre
12. Alena Cherezova
13. Daria V Ilatovskaya
14. James E Bear
15. Neal L Weintraub
16. Eric A Vitriol

Reviewed by Arcadia Science

A Bright, Photostable Dye that Enables Multicolor, Time Lapse, and Super-Resolution Imaging of Acidic Organelles

1. Lauren Lesiak
2. Neville Dadina
3. Shuai Zheng
4. Marianne Schelvis
5. Alanna Schepartz

Reviewed by Arcadia Science

Rab12 is a regulator of LRRK2 and its activation by damaged lysosomes

1. Xiang Wang
2. Vitaliy V Bondar
3. Oliver B Davis
4. Michael T Maloney
5. Maayan Agam
6. Marcus Y Chin
7. Audrey Cheuk-Nga Ho
8. Rajarshi Ghosh
9. Dara E Leto
10. David Joy
11. Meredith EK Calvert
12. Joseph W Lewcock
13. Gilbert Di Paolo
14. Robert G Thorne
15. Zachary K Sweeney
16. Anastasia G Henry

• Curated by eLife

  eLife assessment

  This valuable study shows that Rab12 is required for LRRK2 activation. However, while some of the data are compelling, some claims, especially the ones related to LRRK2's membrane association are not supported. Addressing discrepancies between figures (pointed out by reviewers) and re-writing certain sections will greatly improve this manuscript.

Reviewed by eLife

Synchronization of oscillatory growth prepares fungal hyphae for fusion

1. Valentin Wernet
2. Marius Kriegler
3. Vojtech Kumpost
4. Ralf Mikut
5. Lennart Hilbert
6. Reinhard Fischer

• Curated by eLife

  eLife assessment

  This valuable study combines live cell imaging and mathematical modeling to show how an emerging model fungus engages in an oscillatory chemical dialogue to prepare for cell-cell fusion. Cell data and modeling are in compelling agreement but leave many open questions as to the nature of coordination between cells and the significance of oscillations, rendering the strength of evidence in support of the authors' inferences incomplete.

Reviewed by eLife

Transcription inhibition suppresses nuclear blebbing and rupture independently of nuclear rigidity

1. Isabel K. Berg
2. Marilena L. Currey
3. Sarthak Gupta
4. Yasmin Berrada
5. Bao V. Nguyen
6. Mai Pho
7. Alison E. Patteson
8. J. M. Schwarz
9. Edward J. Banigan
10. Andrew D. Stephens

Reviewed by Review Commons

Centriolar defects underlie a primary ciliary dyskinesia phenotype in an adenylate kinase 7 deficient ciliated epithelium

1. Jennifer Sheridan
2. Aline Grata
3. Julia Dorr
4. Eve E. Suva
5. Enzo Bresteau
6. Linus R. Mitchell
7. Osama Hassan
8. Brian Mitchell

Reviewed by Arcadia Science