Cell Biology

The Long Non-Coding RNA MALAT1 Modulates NR4A1 Expression through a Downstream Regulatory Element in Specific Cancer Cell Types

1. Sara Wernig-Zorc
2. Uwe Schwartz
3. Paulina Martínez-Rodríguez
4. Josefa Inalef
5. Francisca Pavicic
6. Pamela Ehrenfeld
7. Gernot Längst
8. Rodrigo Maldonado

Reviewed by PREreview

Harnessing Nature’s nanoSecrets: biocompatibility, biodistribution and bioactivity of extracellular vesicles derived from microalgae

1. Giorgia Adamo
2. Pamela Santonicola
3. Sabrina Picciotto
4. Paola Gargano
5. Aldo Nicosia
6. Valeria Longo
7. Noemi Aloi
8. Daniele P. Romancino
9. Angela Paterna
10. Estella Rao
11. Samuele Raccosta
12. Rosina Noto
13. Monica Salamone
14. Irene Deidda
15. Salvatore Costa
16. Caterina Di Sano
17. Giuseppina Zampi
18. Svenja Morsbach
19. Katharina Landfester
20. Paolo Colombo
21. Mingxing Wei
22. Paolo Bergese
23. Nicolas Touzet
24. Mauro Manno
25. Elia Di Schiavi
26. Antonella Bongiovanni

Reviewed by Arcadia Science

IntAct: a non-disruptive internal tagging strategy to study actin isoform organization and function

1. M.C. van Zwam
2. A. Dhar
3. W. Bosman
4. W. van Straaten
5. S. Weijers
6. E. Seta
7. B. Joosten
8. S. Palani
9. K. van den Dries

Reviewed by Review Commons, Arcadia Science

The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP

1. Maria Körner
2. Susanne R Meyer
3. Gabriella Marincola
4. Maximilian J Kern
5. Clemens Grimm
6. Christina Schuelein-Voelk
7. Utz Fischer
8. Kay Hofmann
9. Alexander Buchberger

Reviewed by Review Commons

Independent regulation of Z-lines and M-lines during sarcomere assembly in cardiac myocytes revealed by the automatic image analysis software sarcApp

1. Abigail C Neininger-Castro
2. James B Hayes
3. Zachary C Sanchez
4. Nilay Taneja
5. Aidan M Fenix
6. Satish Moparthi
7. Stéphane Vassilopoulos
8. Dylan Tyler Burnette

• Curated by eLife

  eLife assessment

  This manuscript describes a useful tool for quantitative assessment of sarcomere structures in healthy and perturbed cardiomyocytes grown in vitro. The work is solid, and the methods, data and analyses broadly support the claims with only minor weaknesses. The tool will be relevant to biologists working on and interested in obtaining quantitative information on sarcomere structure, function and development.

Reviewed by eLife

Inositol pyrophosphate dynamics reveals control of the yeast phosphate starvation program through 1,5-IP8 and the SPX domain of Pho81

1. Valentin Chabert
2. Geun-Don Kim
3. Danye Qiu
4. Guizhen Liu
5. Lydie Michaillat Mayer
6. Muhammed Jamsheer K
7. Henning J Jessen
8. Andreas Mayer

• Curated by eLife

  eLife assessment

  This fundamental study describes the mechanisms for regulation of the phosphate starvation response in baker's yeast, clarifies the interpretations of prior data, and suggests a unifying mechanism across eukaryotes. The study provides compelling data, based on biochemical analyses, protein localization by fluorescence, and genetic approaches that 1,5-InsP8 is the phosphate nutrient messenger in yeast.

Reviewed by eLife

Selective induction of senescence in cancer cells through near‐infrared light treatment via mitochondrial modulation

1. I. Kalampouka
2. R. R. Mould
3. S. W. Botchway
4. A. M. Mackenzie
5. A. V. Nunn
6. E. L. Thomas
7. J. D. Bell

Reviewed by PREreview

CRB3 navigates Rab11 trafficking vesicles to promote γTuRC assembly during ciliogenesis

1. Bo Wang
2. Zheyong Liang
3. Tan Tan
4. Miao Zhang
5. Yina Jiang
6. Yangyang Shang
7. Xiaoqian Gao
8. Shaoran Song
9. Ruiqi Wang
10. He Chen
11. Jie Liu
12. Juan Li
13. Yu Ren
14. Peijun Liu

• Curated by eLife

  eLife assessment

  This is a useful study for scientists interested in cell polarity, epithelial morphogenesis, cancer, and primary cilia. The authors investigate the role of CRB3 in regulating these processes by using a combination of a mammary epithelial cell-specific conditional Crb3 knockout mouse model, and cellular, molecular and biochemical approaches. The results, which are solid, supporting and extending previous findings, suggest that CRB3 affects ciliogenesis by a mechanism involving Rab11 and gamma-TuRC.

Reviewed by eLife

ESYT1 tethers the endoplasmic reticulum to mitochondria and is required for mitochondrial lipid and calcium homeostasis

1. Alexandre Janer
2. Jordan L. Morris
3. Michiel Krols
4. Hana Antonicka
5. Mari J. Aaltonen
6. Zhen-Yuan Lin
7. Anne-Claude Gingras
8. Julien Prudent
9. Eric A. Shoubridge

Reviewed by Review Commons

PerTurboID, a targeted in situ method reveals the impact of kinase deletion on its local protein environment in the cytoadhesion complex of malaria-causing parasites

1. Heledd Davies
2. Hugo Belda
3. Malgorzata Broncel
4. Jill Dalimot
5. Moritz Treeck

• Curated by eLife

  eLife assessment

  This important study combines conditional mutagenesis with proximity labeling to evaluate alterations in a sub-cellular proteome upon a perturbing event. The approach is applied to the deletion of a kinase involved in trafficking of adhesins to the malaria parasite-infected erythrocyte surface and the evidence supporting the conclusions is compelling. The work will be of broad interest to cell biologists and biochemists.

Reviewed by eLife