Cell Biology

Organelle proteomic profiling reveals lysosomal heterogeneity in association with longevity

1. Yong Yu
2. Shihong M Gao
3. Youchen Guan
4. Pei-Wen Hu
5. Qinghao Zhang
6. Jiaming Liu
7. Bentian Jing
8. Qian Zhao
9. David M Sabatini
10. Monther Abu-Remaileh
11. Sung Yun Jung
12. Meng C Wang

• Curated by eLife

  eLife assessment

  These authors present a powerful tool to unbiasedly identify lysosome-associated proteins in C. elegans, and they provide a compelling, in-depth assessment of how this method can be used to understand longevity pathways and identify novel proteins. Understanding lysosomal differences in specific tissues or in response to different longevity conditions are exciting as it provides new insight into how organelles could control specific homeostasis responses. This valuable tool and proteomics datasets also represent a great resource for the C. elegans community and should pry open new studies on the regulation and role of the lysosome at the organismal level.

Reviewed by eLife

Off-targets of BRAF inhibitors disrupt endothelial signaling and differentially affect vascular barrier function

1. Sophie Bromberger
2. Yuliia Zadorozhna
3. Julia Maria Ressler
4. Silvio Holzner
5. Arkadiusz Nawrocki
6. Nina Zila
7. Alexander Springer
8. Martin Røssel Larsen
9. Klaudia Schossleitner

Reviewed by Review Commons

Tunneling nanotubes enable intercellular transfer in zebrafish embryos

1. Olga Korenkova
2. Shiyu Liu
3. Inès Prlesi
4. Anna Pepe
5. Shahad Albadri
6. Filippo Del Bene
7. Chiara Zurzolo

Reviewed by Arcadia Science

Bsp1, a fungal CPI motif protein, regulates actin filament capping in endocytosis and cytokinesis

1. Daniel R. Hummel
2. Markku Hakala
3. Christopher P. Toret
4. Marko Kaksonen

Reviewed by Arcadia Science

A force-sensitive mutation reveals a spindle assembly checkpoint-independent role for dynein in anaphase progression

1. David Salvador-Garcia
2. Li Jin
3. Andrew Hensley
4. Mert Gölcük
5. Emmanuel Gallaud
6. Sami Chaaban
7. Fillip Port
8. Alessio Vagnoni
9. Vicente José Planelles-Herrero
10. Mark A. McClintock
11. Emmanuel Derivery
12. Andrew P. Carter
13. Régis Giet
14. Mert Gür
15. Ahmet Yildiz
16. Simon L. Bullock

Reviewed by Review Commons

Condensin positioning at telomeres by shelterin proteins drives sister-telomere disjunction in anaphase

1. Léonard Colin
2. Celine Reyes
3. Julien Berthezene
4. Laetitia Maestroni
5. Laurent Modolo
6. Esther Toselli
7. Nicolas Chanard
8. Stephane Schaak
9. Olivier Cuvier
10. Yannick Gachet
11. Stephane Coulon
12. Pascal Bernard
13. Sylvie Tournier

• Curated by eLife

  eLife assessment

  This is an important study that characterises the involvement of condensin complexes in the segregation of telomeres in fission yeast. The authors present convincing evidence to support their claims, employing a diverse range of complementary techniques. This research will be of interest to cell biologists working on chromosome biology and cell division.

Reviewed by eLife

Systemic Lupus Erythematosus Serum Stimulation of Human Intestinal Organoids Induces Changes in Goblet Cell Differentiation and Mitochondrial Fitness

1. Inga Viktoria Hensel
2. Szabolcs Éliás
3. Michelle Steinhauer
4. Bilgenaz Stoll
5. Salvatore Benfatto
6. Wolfgang Merkt
7. Stefan Krienke
8. Hanns-Martin Lorenz
9. Jürgen Haas
10. Brigitte Wildeman
11. Martin Resnik-Docampo

Reviewed by Review Commons

Replisome loading reduces chromatin motion independent of DNA synthesis

1. Maruthi Kumar Pabba
2. Christian Ritter
3. Vadim O Chagin
4. Janis Meyer
5. Kerem Celikay
6. Jeffrey H Stear
7. Dinah Loerke
8. Ksenia Kolobynina
9. Paulina Prorok
10. Alice Kristin Schmid
11. Heinrich Leonhardt
12. Karl Rohr
13. M Cristina Cardoso

• Curated by eLife

  eLife assessment

  This is a valuable investigation of the chromatin dynamics throughout the cell cycle by using fluorescence signals and patterns of GFP-PCNA and CY3-dUTP, which labels newly synthesized DNA. The authors report reduced chromatin mobility in S relative to G1 phase. The technology and methods used are solid. The data will be of interest to researchers working on chromatin dynamics.

Reviewed by eLife

β-Arrestin-dependent and -independent endosomal G protein activation by the vasopressin type 2 receptor

1. Carole Daly
2. Akim Abdul Guseinov
3. Hyunggu Hahn
4. Adam Wright
5. Irina G Tikhonova
6. Alex Rojas Bie Thomsen
7. Bianca Plouffe

• Curated by eLife

  eLife assessment

  This is an important study that contributes to our understanding of the role of beta-arrestins in endosomal activation of the vasopressin type 2 receptors. While the use of a minigene as a tool is a weakness, the evidence is overall convincing and makes for significant findings whose theoretical and practical implications extend to other GPCRs.

Reviewed by eLife

Oral supplementation of gut microbial metabolite indole-3-acetate alleviates diet-induced steatosis and inflammation in mice

1. Yufang Ding
2. Karin Yanagi
3. Fang Yang
4. Evelyn Callaway
5. Clint Cheng
6. Martha E Hensel
7. Rani Menon
8. Robert C Alaniz
9. Kyongbum Lee
10. Arul Jayaraman

• Curated by eLife

  eLife assessment

  The studies are important to the field of hepatic steatosis and inflammation. The data provided are convincing that treatment with I3A mitigated Western diet (WD)-induced hepatic steatosis, inflammation and reversed WD-induced alterations in liver bile acids and free fatty acids in mice.

Reviewed by eLife