Cell Biology

Development and validation of a high throughput screening platform to enable target identification in skeletal muscle cells from Duchenne Muscular Dystrophy (DMD) patients

1. Santosh Hariharan
2. Oana Lorintiu
3. Chia-Chin Lee
4. Eve Duchemin-Pelletier
5. Xianfeng Li
6. Aileen Healy
7. Regis Doyonnas
8. Luc Selig
9. Pauline Poydenot
10. Erwann Ventre
11. Andrea Weston
12. Jane Owens
13. Nicolas Christoforou

• Curated by eLife

  eLife assessment

  This is a solid methods paper developing a machine learning based protocol differentiating normal and diseased myofibers. It emerges with and validates a potentially valuable approach to diifferentiate healthy and Duchenne muscle dystrophy myofibers.

Reviewed by eLife

Crosstalk between the plasma membrane and cell-cell adhesion maintains epithelial identity for correct polarised cell divisions

1. Manal M. Hosawi
2. Jiaoqi Cheng
3. Maria Fankhaenel
4. Marcin R. Przewloka
5. Salah Elias

Reviewed by Review Commons

Phosphorylation-dependent interactions of VAPB and ELYS contribute to the temporal progression of mitosis

1. Christina James
2. Ulrike Möller
3. Sabine König
4. Henning Urlaub
5. Ralph H. Kehlenbach

Reviewed by Review Commons

Remodeling of the secretory pathway is coordinated with de novo membrane formation in budding yeast gametogenesis

1. Yasuyuki Suda
2. Hiroyuki Tachikawa
3. Tomomi Suda
4. Kazuo Kurokawa
5. Akihiko Nakano
6. Kenji Irie

Reviewed by Review Commons

Heterogeneity of radial spokes structural components and associated enzymes in Tetrahymena cilia

1. Marta Bicka
2. Corbin Black
3. Avrin Ghanaeian
4. Ewa Joachimiak
5. Anna Osinka
6. Sumita Majhi
7. Anna Konopka
8. Ewa Bulska
9. Khanh Huy Bui
10. Dorota Wloga

• Curated by eLife

  eLife assessment

  This study presents valuable new insights from the protist Tetrahymena regarding radial spokes, conserved protein complexes that are relevant for cilia motility. The work employs interdisciplinary approaches to provide convincing support for radial spoke composition with some experiments, but there are weaker areas with partially incomplete support, such as relying on knockouts alone rather than including localization studies of tagged proteins.

Reviewed by eLife

Continuous sensing of nutrients and growth factors by the mTORC1-TFEB axis

1. Breanne Sparta
2. Nont Kosaisawe
3. Michael Pargett
4. Madhura Patankar
5. Nicholaus DeCuzzi
6. John G Albeck

• Curated by eLife

  eLife assessment

  In this study, Sparta et al., generated and employed a battery of fluorescent reporters that allowed them to perform time-resolved monitoring of mechanistic target of rapamycin complex 1 (mTORC1) responses to stimuli including glucose, amino acids, and insulin at the single cell resolution. The results of this elegant approach support a model of graded mTORC1 activation in response to the aforementioned stimuli when applied individually or in combination. This model is consistent with continuous adjustment of mTORC1 signaling to changes in cellular environment and opposed to the "on/off" model of mTORC1 function. Considering the pivotal role of mTORC1 in integrating signals such as nutrients, hormones, growth factors, oxygen, and energy status with a plethora of outputs that affect cell fate and organismal physiology, it was thought that this study will be of interests across a variety of biomedical disciplines. Overall, the elegance and robustness of the approach was highly appreciated, though the paper would be strengthened by addressing some technical issues and concerns regarding the positioning of the proposed model of mTORC1 regulation in the field.

Reviewed by eLife

Mechano-dependent sorbitol accumulation supports biomolecular condensate

1. Stephanie Torrino
2. William M. Oldham
3. Andrés R. Tejedor
4. Ignacio S. Burgos
5. Lara Nasr
6. Nesrine Rachedi
7. Kéren Fraissard
8. Caroline Chauvet
9. Chaima Sbai
10. Brendan P. O’Hara
11. Sophie Abélanet
12. Frederic Brau
13. Cyril Favard
14. Stephan Clavel
15. Rosana Collepardo-Guevara
16. Jorge R. Espinosa
17. Issam Ben-Sahra
18. Thomas Bertero

Reviewed by preLights

Fluorescein-based sensors to purify human α-cells for functional and transcriptomic analyses

1. Sevim Kahraman
2. Kimitaka Shibue
3. Dario F De Jesus
4. Hyunki Kim
5. Jiang Hu
6. Debasish Manna
7. Bridget Wagner
8. Amit Choudhary
9. Rohit N Kulkarni

• Curated by eLife

  eLife assessment

  The manuscript by Kahraman et al. describes the use of a fluorescent dye for purifying and analyzing human islet alpha cells. The study provides solid evidence that the alpha cells can be purified using this method and the cells remained viable and functional after culturing for several days. The significance of the study is access to a new tool that will be useful for islet biologists and researchers studying diabetes mechanisms.

Reviewed by eLife

Tip60-mediated Rheb acetylation links palmitic acid with mTORC1 activation and insulin resistance

1. Zengqi Zhao
2. Qiang Chen
3. Xiaojun Xiang
4. Weiwei Dai
5. Wei Fang
6. Kun Cui
7. Baolin Li
8. Qiangde Liu
9. Yongtao Liu
10. Yanan Shen
11. Yueru Li
12. Wei Xu
13. Kangsen Mai
14. Qinghui Ai

Reviewed by Review Commons

Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast

1. Takuro Tojima
2. Yasuyuki Suda
3. Natsuko Jin
4. Kazuo Kurokawa
5. Akihiko Nakano

Reviewed by Review Commons