Cell Biology

GLUD1 dictates muscle stem cell differentiation by controlling mitochondrial glutamate levels

1. Inés Soro-Arnáiz
2. Sarah Cherkaoui
3. Gillian Fitzgerald
4. Jing Zhang
5. Paola Gilardoni
6. Adhideb Ghosh
7. Ori Bar-Nur
8. Evi Masschelein
9. Pierre Maechler
10. Nicola Zamboni
11. Martin Poms
12. Alessio Cremonesi
13. Juan Carlos García Cañaveras
14. Katrien De Bock
15. Raphael J. Morscher

Reviewed by PREreview

Microtubule reorganization during mitotic cell division in the dinoflagellate Ostreospis cf. ovata

1. David Velasquez-Carvajal
2. Flavie Garampon
3. Rodolphe Lemée
4. Sebastian Schaub
5. Stefania Castagnetti

Reviewed by Arcadia Science

Distinct states of nucleolar stress induced by anticancer drugs

1. Tamara A Potapova
2. Jay R Unruh
3. Juliana Conkright-Fincham
4. Charles AS Banks
5. Laurence Florens
6. David Alan Schneider
7. Jennifer L Gerton

• Curated by eLife

  eLife assessment

  This study and associated data is compelling, novel, important, and well-carried out. The study demonstrates a novel finding that different chemotherapeutic agents can induce nucleolar stress, which manifests with varying cellular and molecular characteristics. The study also proposes a mechanism for how a novel type of nucleolar stress driven by CDK inhibitors may be regulated. The study sheds light on the importance of nucleolar stress in defining the on-target and off-target effects of chemotherapy in normal and cancer cells.

Reviewed by eLife

A Window into Mammalian Basement Membrane Development: Insights from the mTurq2-Col4a1 Mouse Model

1. Rebecca A. Jones
2. Brandon Trejo
3. Parijat Sil
4. Katherine A. Little
5. H. Amalia Pasolli
6. Bradley Joyce
7. Eszter Posfai
8. Danelle Devenport

Reviewed by preLights

Competition and synergy of Arp2/3 and formins in nucleating actin waves

1. Xiang Le Chua
2. Chee San Tong
3. Maohan Su
4. X.J. Xǔ
5. Shengping Xiao
6. Xudong Wu
7. Min Wu

Reviewed by preLights

Interface-acting nucleotide controls polymerization dynamics at microtubule plus- and minus-ends

1. Lauren A McCormick
2. Joseph M Cleary
3. William O Hancock
4. Luke M Rice

• Curated by eLife

  eLife assessment

  This important study combines in vitro experiments with simulations to identify the mechanisms governing modulation of microtubule dynamics by GTP hydrolysis. The authors introduce a convincing new approach by using a mixed GDP/GMPCPP lattice and varying GDP concentration to reveal that the nucleotide at the interface of two tubulin dimers determines the strength of the interaction between two dimers. Overall, the findings will be of interest to biophysicists and cell biologists, especially in the field of microtubule biology.

Reviewed by eLife

ARHGAP18-ezrin functions as an autoregulatory module for RhoA in the assembly of distinct actin-based structures

1. Andrew T Lombardo
2. Cameron AR Mitchell
3. Riasat Zaman
4. David J McDermitt
5. Anthony Bretscher

• Curated by eLife

  eLife assessment

  This fundamental work substantially advances our understanding of how cells can tightly modulate small GTPase activity to build and maintain neighboring cytoskeletal structures, in this case microvilli. The evidence supporting these claims is compelling and is supported by both protein-protein interaction assays as well as cell biological studies. The work will be of interest to cell biologist studying the cytoskeleton as well as those interested in G-protein mediated regulation.

Reviewed by eLife

Differential regulation of hair cell actin cytoskeleton mediated by SRF and MRTFB

1. Ling-Yun Zhou
2. Chen-Xi Jin
3. Wen-Xiao Wang
4. Lei Song
5. Jung-Bum Shin
6. Ting-Ting Du
7. Hao Wu

• Curated by eLife

  eLife assessment

  This valuable study provides solid evidence implicating two transcription factors in the development of the actin cytoskeleton that shapes the mechanosensory hair bundles of the inner ear's hair cells. The work will be of interest to biologists interested in the development and maintenance of the hair bundle, both normal or impaired. Its impact would be improved by providing a mechanistic model for the observed effects.

Reviewed by eLife

Organelle proteomic profiling reveals lysosomal heterogeneity in association with longevity

1. Yong Yu
2. Shihong M Gao
3. Youchen Guan
4. Pei-Wen Hu
5. Qinghao Zhang
6. Jiaming Liu
7. Bentian Jing
8. Qian Zhao
9. David M Sabatini
10. Monther Abu-Remaileh
11. Sung Yun Jung
12. Meng C Wang

• Curated by eLife

  eLife assessment

  These authors present a powerful tool to unbiasedly identify lysosome-associated proteins in C. elegans, and they provide a compelling, in-depth assessment of how this method can be used to understand longevity pathways and identify novel proteins. Understanding lysosomal differences in specific tissues or in response to different longevity conditions are exciting as it provides new insight into how organelles could control specific homeostasis responses. This valuable tool and proteomics datasets also represent a great resource for the C. elegans community and should pry open new studies on the regulation and role of the lysosome at the organismal level.

Reviewed by eLife

Off-targets of BRAF inhibitors disrupt endothelial signaling and differentially affect vascular barrier function

1. Sophie Bromberger
2. Yuliia Zadorozhna
3. Julia Maria Ressler
4. Silvio Holzner
5. Arkadiusz Nawrocki
6. Nina Zila
7. Alexander Springer
8. Martin Røssel Larsen
9. Klaudia Schossleitner

Reviewed by Review Commons