Cell Biology

Exonuclease Xrn1 regulates TORC1 signaling in response to SAM availability

1. Madeline M. McGinnis
2. Benjamin M. Sutter
3. Samira Jahangiri
4. Benjamin P. Tu

• Curated by eLife

  eLife assessment

  This study in budding yeast (S. cerevisiae) presents important findings demonstrating that the exonuclease Xrn1 regulates autophagy in response to methionine deprivation through effects on TORC1. There is solid evidence that the impact of Xrn1 on TORC1 is contingent on its catalytic activity rather than the degradation of any specific category of mRNAs. A major strength is the novel mechanism, in which Xrn1 modulates the nucleotide-binding state of the Gtr1/2 complex.

Reviewed by eLife

The stress-induced lincRNA JUNI is a critical factor for cancer cell survival whose interactome is a prognostic signature in clear cell renal cell carcinoma

1. Vikash Kumar
2. Xavier Sabaté-Cadenas
3. Isha Soni
4. Esther Stern
5. Carine Vias
6. Doron Ginsberg
7. Carlos Romá-Mateo
8. Rafael Pulido
9. Martin Dodel
10. Faraz K. Mardakheh
11. Iddo Z. Ben-Dov
12. Alena Shkumatava
13. Eitan Shaulian

Reviewed by Review Commons

Structured RhoGEF recruitment drives myosin II organization on large exocytotic vesicles

1. Kumari Kamalesh
2. Dagan Segal
3. Ori Avinoam
4. Eyal D. Schejter
5. Ben-Zion Shilo

Reviewed by preLights

Phosphorylation bar-coding of free fatty acid receptor 2 is generated in a tissue-specific manner

1. Natasja Barki
2. Laura Jenkins
3. Sara Marsango
4. Domonkos Dedeo
5. Daniele Bolognini
6. Louis Dwomoh
7. Aisha M Abdelmalik
8. Margaret Nilsen
9. Manon Stoffels
10. Falko Nagel
11. Stefan Schulz
12. Andrew B Tobin
13. Graeme Milligan

• Curated by eLife

  eLife assessment

  In this study, the authors present important tools for monitoring distinct tissue-specific patterns of agonist-induced Free Fatty Acid receptor 2 phosphorylation. The work includes several validation experiments, which provide convincing evidence that will be beneficial for the scientific community.

Reviewed by eLife

Mechanical coupling coordinates microtubule growth

1. Bonnibelle K Leeds
2. Katelyn F Kostello
3. Yuna Y Liu
4. Christian R Nelson
5. Sue Biggins
6. Charles L Asbury

• Curated by eLife

  eLife assessment

  In this technically advanced and important piece of work, the authors study the coordination of microtubule growth in kinetochore fibers using force spectroscopy and numerical simulations. With compelling evidence the authors address the question of how microtubules, which naturally exhibit variable growth rates, can coordinate their behavior by mechanical coupling so as to function as a single unit in generating forces during chromosome segregation.

Reviewed by eLife

Metabolic regulation of misfolded protein import into mitochondria

1. Yuhao Wang
2. Linhao Ruan
3. Jin Zhu
4. Xi Zhang
5. Alexander Chih-Chieh Chang
6. Alexis Tomaszewski
7. Rong Li

• Curated by eLife

  eLife assessment

  This study makes a connection between cellular metabolism and proteostasis through MAGIC, a previously proposed protein quality control pathway of clearance of cytosolic misfolded and aggregated proteins by importing into mitochondria. The authors reveal the role of Snf1, a yeast AMPK, in preventing the import of misfolded proteins to mitochondria for MAGIC controlled by the transcription factor Hap4, depending on the cellular metabolic status. The key message is important, although the evidence for physiological relevance of MAGIC for overall cellular proteostasis and its molecular regulation by Snf1 remains incomplete.

Reviewed by eLife, Life Science Editors Foundation

The loss of DNA polymerase epsilon accessory subunits POLE3-POLE4 leads to BRCA1-independent PARP inhibitor sensitivity

1. Hasan Mamar
2. Roberta Fajka-Boja
3. Mónika Mórocz
4. Eva Pinto Jurado
5. Siham Zentout
6. Alexandra Mihuț
7. Anna Georgina Kopasz
8. Mihály Mérey
9. Rebecca Smith
10. Lajos Haracska
11. Sébastien Huet
12. Gyula Timinszky

Reviewed by preLights

Tight nanoscale clustering of Fcγ receptors using DNA origami promotes phagocytosis

1. Nadja Kern
2. Rui Dong
3. Shawn M Douglas
4. Ronald D Vale
5. Meghan A Morrissey

Reviewed by Review Commons, preLights

Mild mitochondrial impairment enhances innate immunity and longevity through ATFS‐1 and p38 signaling

1. Juliane C Campos
2. Ziyun Wu
3. Paige D Rudich
4. Sonja K Soo
5. Meeta Mistry
6. Julio CB Ferreira
7. T Keith Blackwell
8. Jeremy M Van Raamsdonk

Reviewed by Review Commons

Genetic screen identified PRMT5 as a neuroprotection target against cerebral ischemia

1. Haoyang Wu
2. Peiyuan Lv
3. Jinyu Wang
4. Brian Bennett
5. Jiajia Wang
6. Pishun Li
7. Yi Peng
8. Guang Hu
9. Jiaji Lin

• Curated by eLife

  eLife assessment

  The authors performed a useful RNAi screen to identify epigenetic regulators involved in oxygen-glucose deprivation (OGD)-induced neuronal injury. PRMT5 was identified as a negative regulator of neuronal cell survival after OGD. Solid in vitro and in vivo data suggest that PRMT5 could be a novel therapeutic target for the treatment of ischemic stroke.

Reviewed by eLife