eLife

Latest preprint reviews

1. PHD1-dependent hydroxylation of RepoMan (CDCA2) on P604 modulates the control of mitotic progression

   This article has 12 authors:

   1. Jimena Druker
   2. Hao Jiang
   3. Dilem Shakir
   4. Fraser Child
   5. Vanesa Alvarez
   6. Melpomeni Platani
   7. Andrea Corno
   8. Constance Alabert
   9. Adrian T Saurin
   10. Jason R Swedlow
   11. Sonia Rocha
   12. Angus I Lamond

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This compelling work describes how the cell cycle-regulating phosphatase subunit, RepoMan, is regulated by the oxygen-dependent, metabolite-sensing hydroxylase PHD1. The characterisation of how proline hydroxylation alters signalling at the molecular and cellular level provides important evidence to enhance our understanding of how 2-oxoglutarate-dependent dioxygenases influence the cell cycle and mitosis.

   Reviewed by eLife

2. SLC4A1 mutations that cause distal renal tubular acidosis alter cytoplasmic pH and cellular autophagy

   This article has 12 authors:

   1. Grace Essuman
   2. Midhat Rizvi
   3. Ensaf Almomani
   4. Shahid AK M Ullah
   5. Sarder MA Hasib
   6. Forough Chelangarimiyandoab
   7. Priyanka Mungara
   8. Manfred J Schmitt
   9. Marguerite Hureaux
   10. Rosa Vargas-Poussou
   11. Nicolas Touret
   12. Emmanuelle Cordat

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work reports the characterization of newly identified genetic variants of SLC4A1 in patients with distal renal tubular acidosis. Cell culture studies supplemented with histological analysis of a previously established disease mouse model provide convincing evidence that some of the variants increase intracellular pH, reduce ATP synthesis, and attenuate autophagic degradative flux. The study is valuable in establishing a mechanistic framework for future exploration of the link between intracellular pH and mutations in SLC4A1 in vivo.

   Reviewed by eLife

3. Optimised genome editing for precise DNA insertion and substitution using Prime Editors in zebrafish

   This article has 11 authors:

   1. Yosuke Ono
   2. Martin Peterka
   3. Michael Love
   4. Ashish Bhandari
   5. Euan Gordon
   6. Jonathan S Ball
   7. Charles R Tyler
   8. Steve Rees
   9. Mohammad Bohlooly-Y
   10. Marcello Maresca
   11. Steffen Scholpp

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a valuable comparison of the efficiency and precision of two prime editing methods to introduce single-nucleotide variants and longer exogenous DNA sequences into the zebrafish genome. Solid data support the conclusion that the PE2 prime editor Nickase is more effective at introducing single-nucleotide variants, while the PEn prime editor nuclease is more effective at integrating short sequences from 3 up to 30 base pairs, for both somatic and germline editing. The results will be of interest to the zebrafish community, in particular to model human disease variants in this model organism.

   Reviewed by eLife

4. Decoding movie content from neuronal population activity in the human medial temporal lobe

   This article has 12 authors:

   1. Franziska Gerken
   2. Alana Darcher
   3. Pedro J Gonçalves
   4. Rachel Rapp
   5. Ismail Elezi
   6. Johannes Niediek
   7. Marcel S Kehl
   8. Thomas P Reber
   9. Stefanie Liebe
   10. Jakob H Macke
   11. Florian Mormann
   12. Laura Leal-Taixé

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study demonstrates that it is possible to decode information about characters and locations from single-unit responses in the human brain to a narrative movie, using a convincing technical approach to capture information in population-level dynamics. The study introduces an exciting dataset of single-unit responses in humans during a naturalistic and dynamic movie stimulus, with recordings from multiple regions within the medial temporal lobe. Using both a traditional firing-rate analysis as well as a population decoding analysis to connect these neural responses to the visual content of the movie, they show that in this dataset, the decoding of semantic scene features (e.g., the person currently on screen), but not scene transitions, is surprisingly driven by classically non-responsive neurons. Based on these findings, the authors argue that dynamic naturalistic semantic information may be processed within the medial temporal lobe at the population level.

   Reviewed by eLife

5. Specific GPCRs Elicit Unique Extracellular Vesicle MiRNA Array Signatures: An Exploratory Study

   This article has 6 authors:

   1. Xiao Shi
   2. Michelle C Palumbo
   3. Sheila Benware
   4. Jack Wiedrick
   5. Sheila Markwardt
   6. Aaron Janowsky

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents valuable findings by demonstrating that specific GPCR subtypes induce distinct extracellular vesicle miRNA signatures, highlighting a potential novel mechanism for intercellular communication with implications for receptor pharmacology within the field. The evidence is solid, however, more experiments are needed to determine whether the distinct extracellular vesicle miRNA signatures result from GPCR-dependent miRNA expression or GPCR-dependent incorporation of miRNAs into extracellular vesicles.

   Reviewed by eLife

6. New idtracker.ai: rethinking multi-animal tracking as a representation learning problem to increase accuracy and reduce tracking times

   This article has 3 authors:

   1. Jordi Torrents
   2. Tiago Costa
   3. Gonzalo G de Polavieja

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study introduces an advance in multi-animal tracking by reframing identity assignment as a self-supervised contrastive representation learning problem. It eliminates the need for segments of video where all animals are simultaneously visible and individually identifiable, and significantly improves tracking speed, accuracy, and robustness with respect to occlusion. This innovation has implications beyond animal tracking, potentially connecting with advances in behavioral analysis and computer vision. The strength of support for these advances is compelling overall, although there were some remaining minor methodological concerns.

   Reviewed by eLife

7. A rapid transfer of virions coated with heparan sulfate from the ECM to CD151 defines an early step in the human papillomavirus infection cascade

   This article has 7 authors:

   1. Annika Massenberg
   2. Yahya Homsi
   3. Carl Niklas Schneider
   4. Snježana Mikuličić
   5. Tatjana Döring
   6. Luise Florin
   7. Thorsten Lang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable insight into the role of actin protrusions in mediating early pre-endoyctic steps of human papillomavirus entry at the cell surface. Using state-of-the-art microscopy in an immortalized keratinocyte model, the authors present mostly solid evidence that filopodia actively promote the transfer of heparin sulfate-coated virions from the extracullar matrix to the viral entry factor CD151. Remaining gaps in the mechanistic model could be further supported by including a more expansive analysis of the fixed microscopy samples and live cell imaging to distinguish virion transfer from direct binding.

   Reviewed by eLife

8. Evolution of gene expression in seasonal environments

   This article has 6 authors:

   1. Shuichi N Kudo
   2. Yuka Ikezaki
   3. Junko Kusumi
   4. Hideki Hirakawa
   5. Sachiko Isobe
   6. Akiko Satake

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors collected time-course RNA-seq data from four tree species in natural environments and analyzed seasonal patterns of gene expression. This fundamental study substantially advances our understanding of how seasonal environments shape gene expression. The evolutionary effects of seasonal environments on gene expression are rarely studied at this scale and the dataset is extensive. The evidence supporting the conclusions is compelling, with caveats and limitations clearly described. The work will be of broad interest to colleagues studying evolution and gene expression.

   Reviewed by eLife

9. Efficient Generation of Expandable Dorsal Forebrain Neural Rosette Stem Cell Lines

   This article has 9 authors:

   1. Signe Emilie Dannulat Frazier
   2. Kristian Honnens de Lichtenberg
   3. Elham Jaberi
   4. Charlotte Bertelsen
   5. Simone Møller Jensen
   6. Andreas Wrona
   7. Nicolaj Strøyer Christophersen
   8. Mie Kristensen
   9. J Carlos Villaescusa

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The study provides valuable technical advances to generate and isolate neural rosettes. The technique is robust, as indicated by both reviewers. The evidence is solid, as shown in orthogonal characterization by flow cytometry, morphology, and scRNA-seq. Comparison with the manual-rosette-picking protocol will enhance the validity of the claims.

   Reviewed by eLife

10. Repeated vaccination with homologous influenza hemagglutinin broadens human antibody responses to unmatched flu viruses

    This article has 6 authors:

    1. Yixiang Deng
    2. Melbourne Tang
    3. Ted M Ross
    4. Aaron G Schmidt
    5. Arup K Chakraborty
    6. Daniel Lingwood

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This valuable study provides outlines the mechanism by which repeated vaccination broadens the breadth of antibody responses against epitope unmatched virus strains. The authors' mathematical model is solid and incorporates various parameters that regulate B cell activation and antibody response.

    Reviewed by eLife