eLife

Latest preprint reviews

1. Multiple antagonist calcium-dependent mechanisms control CaM kinase-1 subcellular localization in a C. elegans thermal nociceptor

   This article has 2 authors:

   1. Domenica Ippolito
   2. Dominique A Glauser

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable study provides solid results on the molecular signaling mechanisms of CaM kinase kinase-1 (CKK-1) in the context of the nociceptive behaviors of C. elegans. The authors report previously undescribed elements that control the nuclear/cytoplasmic shuttling of CKK-1, suggesting a complex interplay of multiple nuclear localization and export sequences. Therefore, the work will be of broad interest to scientists studying behavior, neuronal signaling, and signal transduction in general.

   Reviewed by eLife

2. Variation in thermal physiology can drive the temperature-dependence of microbial community richness

   This article has 2 authors:

   1. Tom Clegg
   2. Samraat Pawar

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study proposes a phenomenologically motivated theoretical framework to explain observed patterns of the temperature dependence of microbial diversity. The methodology is overall convincing, but the explanations of approximations and assumptions, and of their regime of validity, are incomplete. The manuscript should be of interest to microbial ecologists.

   Reviewed by eLife

3. Osteoblast-intrinsic defect in glucose metabolism impairs bone formation in type II diabetic male mice

   This article has 10 authors:

   1. Fangfang Song
   2. Won Dong Lee
   3. Tyler Marmo
   4. Xing Ji
   5. Chao Song
   6. Xueyang Liao
   7. Rebecca Seeley
   8. Lutian Yao
   9. Haoran Liu
   10. Fanxin Long

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this study, the authors made important progress in understanding bone metabolic defects of T2D. They have established a valuable model that could mimic some aspects of T2D in mice. Particularly, the study provided important evidence showing bone turnover and metabolism were in defects, and changes in glycolysis would rescue bone defects in T2D. Overall, the authors provide compelling evidence from dynamic histomorphometry, C13 isotype labeling in vivo, scRNA-seq, and metabolic assays to demonstrate that the defective glucose metabolism causes osteopenia associated with T2D.

   Reviewed by eLife

4. Antisense, but not sense, repeat expanded RNAs activate PKR/eIF2α-dependent ISR in C9ORF72 FTD/ALS

   This article has 15 authors:

   1. Janani Parameswaran
   2. Nancy Zhang
   3. Elke Braems
   4. Kedamawit Tilahun
   5. Devesh C Pant
   6. Keena Yin
   7. Seneshaw Asress
   8. Kara Heeren
   9. Anwesha Banerjee
   10. Emma Davis
   11. Samantha L Schwartz
   12. Graeme L Conn
   13. Gary J Bassell
   14. Ludo Van Den Bosch
   15. Jie Jiang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The current study provides important, mechanistic insight into the potential contribution of antisense C4G2 expanded RNA to disease in C9orf72-associated ALS/FTD. The authors convincingly demonstrate that expression of this RNA species activates the PKR/eIF2α-dependent integrated stress response. They further provide evidence that this can contribute to disease phenotypes using multiple models and post-mortem patient samples.

   Reviewed by eLife

5. Staphylococcus aureus FtsZ and PBP4 bind to the conformationally dynamic N-terminal domain of GpsB

   This article has 14 authors:

   1. Michael D Sacco
   2. Lauren R Hammond
   3. Radwan E Noor
   4. Dipanwita Bhattacharya
   5. Lily J McKnight
   6. Jesper J Madsen
   7. Xiujun Zhang
   8. Shane G Butler
   9. M Trent Kemp
   10. Aiden C Jaskolka-Brown
   11. Sebastian J Khan
   12. Ioannis Gelis
   13. Prahathees Eswara
   14. Yu Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study reports a unique N-terminal motif of Staphylococcus aureus GpsB and the co-crystal structure of GpsB with the C-terminus of PBP4. It provides convincing evidence demonstrating the interactions of GpsB with PBP4 and FtsZ, shedding light on the role of GpsB in the pathogen's cell division. However, the functional characterization of GpsB's new motif caused and the structural characterization of GpsB and FtsZ's interaction is incomplete.

   Reviewed by eLife

6. Evidence for absence of links between striatal dopamine synthesis capacity and working memory capacity, spontaneous eye-blink rate, and trait impulsivity

   This article has 9 authors:

   1. Ruben van den Bosch
   2. Frank H Hezemans
   3. Jessica I Määttä
   4. Lieke Hofmans
   5. Danae Papadopetraki
   6. Robbert-Jan Verkes
   7. Andre F Marquand
   8. Jan Booij
   9. Roshan Cools

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental work shows the absence of links between striatal dopamine synthesis capacity and working memory capacity, spontaneous eye-blink rate, and trait impulsivity. The evidence supporting the conclusions is compelling, with rigorous PET investigations and state-of-the-art cognitive assessments in a large sample. Given the high interest in the role of dopamine, the work will be of very broad interest to basic neuroscientists, clinical neuroscientists, and clinicians including neurologists and psychiatrists.

   Reviewed by eLife

7. Defects in lipid homeostasis reflect the function of TANGO2 in phospholipid and neutral lipid metabolism

   This article has 10 authors:

   1. Agustin Leonardo Lujan
   2. Ombretta Foresti
   3. Conor Sugden
   4. Nathalie Brouwers
   5. Alex Mateo Farre
   6. Alessio Vignoli
   7. Mahshid Azamian
   8. Alicia Turner
   9. Jose Wojnacki
   10. Vivek Malhotra

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important manuscript describes a series of cellular phenotypes associated with the depletion of TANGO2, a poorly characterized gene product but relevant to neurological and muscular disorders. The authors present solid data indicating that TANGO2 associates with membrane-bound organelles, mainly mitochondria, impacting lipid metabolism and the accumulation of reactive-oxygen species. A few additional experiments would help to understand the link between the lipid changes reported and the cellular phenotype.

   Reviewed by eLife

8. Microcephaly-associated WDR62 mutations hamper Golgi apparatus-to-spindle pole shuttling in human neural progenitors

   This article has 10 authors:

   1. Claudia Dell’Amico
   2. Marilyn M. Angulo Salavarria
   3. Yutaka Takeo
   4. Ichiko Saotome
   5. Maria Teresa Dell’Anno
   6. Maura Galimberti
   7. Enrica Pellegrino
   8. Elena Cattaneo
   9. Angeliki Louvi
   10. Marco Onorati

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The paper is of interest to neuroscientists, developmental biologists, and those interested in mechanisms that underlie intellectual disability. The study is well executed and brings new insight into the role of WDR62 and its role in causing microcephaly. The key claims of the manuscript require additional data.

   Reviewed by eLife

9. Allele-specific gene-editing approach for vision loss restoration in RHO-associated retinitis pigmentosa

   This article has 7 authors:

   1. Xiaozhen Liu
   2. Jing Qiao
   3. Ruixuan Jia
   4. Fan Zhang
   5. Xiang Meng
   6. Yang Li
   7. Liping Yang

   This article has been curated by 1 group:

   • Curated by eLife

     **eLife assessment
     **
     This work provides a valuable allele-specific gene editing therapeutic approach to selectively target the human RHO-T17M mutation, one of the most frequent genetic causes of autosomal dominant retinitis pigmentosa. However, the current data are incomplete. Further validation of gene editing efficiency in rods at cellular level in vivo and use of Rho-T17M mice will strengthen the conclusion.

   Reviewed by eLife

10. Lifelong regeneration of cerebellar Purkinje cells after induced cell ablation in zebrafish

    This article has 6 authors:

    1. Sol Pose-Méndez
    2. Paul Schramm
    3. Barbara Winter
    4. Jochen C Meier
    5. Konstantinos Ampatzis
    6. Reinhard W Köster

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      The present manuscript addresses the controversial issue of the regeneration potential of cerebellar Purkinje cells in zebrafish and their integration into functional circuits. The authors use interesting genetic models to induce Purkinje cell-specific ablation to demonstrate regeneration of Purkinje cells can occur until adulthood and is accomplished by ptf1a+ progenitors. They further show that regenerated neurons reestablish electrophysiological properties and support appropriate behavior. These are important results that may help understand why mammalian neurons do not have similar properties and fail to regenerate. The conclusions on the source of regenerated neurons will however need additional experimental support.

    Reviewed by eLife