eLife

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1. The title and authors for this article are not available from our external data provider yet:
   10.48550/arxiv.2402.07252v3

2. Activity-dependent synapse elimination requires caspase-3 activation

   This article has 4 authors:

   1. Zhou Yu
   2. Andrian Gutu
   3. Namsoo Kim
   4. Erin K O'Shea

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents an important finding on the involvement of a Caspase 3-dependent pathway in the elimination of synapses for retinogeniculate circuit refinement and eye-specific territory segregation. This work fits well with the concept of "synaptosis" which has been proposed in the past. The evidence supporting the claims of the authors is convincing, demonstrating that caspase-3 activation is essential for microglial elimination of synapses during both brain development and neurodegeneration. The work will be of interest to investigators studying cell death pathways, neurodevelopment, and neurodegenerative disease.

   Reviewed by eLife

3. Characterization of direct Purkinje cell outputs to the brainstem

   This article has 4 authors:

   1. Christopher H Chen
   2. Zhiyi Yao
   3. Shuting Wu
   4. Wade G Regehr

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important paper provides an unbiased landscape for the cerebellar cortical outputs to the brainstem nuclei. By conducting anatomical and physiological analyses of the axonal terminals of Purkinje cells, the data provides convincing evidence that Purkinje cells innervate brainstem nuclei directly. The results show that in addition to previously known inputs to vestibular and parabrachial nuclei, Purkinje cells synapse onto the pontine central grey nucleus but have little effect on the locus coeruleus and mesencephalic trigeminal neurons.

   Reviewed by eLife

4. Xist RNA binds select autosomal genes and depends on Repeat B to regulate their expression

   This article has 4 authors:

   1. Shengze Yao
   2. Yesu Jeon
   3. Barry Kesner
   4. Jeannie T Lee

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study addresses the potential roles of the master regulator of X chromosome inactivation, the Xist long non-coding RNA, in the regulation of autosomal genes. Using data from mouse cells, the authors propose that Xist can coat specific autosomal promoters, which in turn leads to the attenuation of their transcriptional activity. The evidence from individual genes is interesting, and the model aligns with recently published results from humans. However, despite some improvements during revision, the data and statistical analyses in the current study are not yet strong enough to allow for conclusive inferences, leaving the evidence for mouse cells behaving like human cells incomplete. The topic of the work is of broad interest, in particular to colleagues studying gene regulation and noncoding RNAs.

   Reviewed by eLife

5. Crystal structure and catalytic mechanism of PL35 family glycosaminoglycan lyases with an ultrabroad substrate spectrum

   This article has 12 authors:

   1. Lin Wei
   2. Hai-Yan Cao
   3. Ruyi Zou
   4. Min Du
   5. Qingdong Zhang
   6. Danrong Lu
   7. Xiangyu Xu
   8. Yingying Xu
   9. Wenshuang Wang
   10. Xiu-Lan Chen
   11. Yu-Zhong Zhang
   12. Fuchuan Li

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This useful manuscript reports on the crystal structures of two glycosaminoglycan (GAG) lyases from the PL35 family, along with in vitro enzyme activity assays and comprehensive structure-guided mutagenesis. The authors have addressed key concerns by incorporating additional docking analyses, validating the role of His188 in alginate degradation, and providing ICP-MS data to examine Mn²⁺ binding. While these improvements enhance the study, the study is incomplete due to the lack of enzyme-substrate complex structures and reliance on modeling which still limit mechanistic insight. Nonetheless, the revised manuscript presents a more complete analysis that will be of interest to specialists in carbohydrate-active enzymes.

   Reviewed by eLife

6. LAPTM4B Alleviates Pulmonary Fibrosis by Enhancing NEDD4L-Mediated TGF-β Signaling Suppression

   This article has 12 authors:

   1. Kai Xu
   2. Xiaoyue Pan
   3. Hui Lian
   4. Yaxuan Wang
   5. Ruyan Wan
   6. Zhongzheng Li
   7. Xin Pan
   8. Yajun Li
   9. Juntang Yang
   10. Ivan Rosas
   11. Lan Wang
   12. Guoying Yu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this valuable manuscript, the authors propose that the lysosomal protein LAPTM4B plays a role in suppressing the TGF-β/SMAD signaling pathway and suggest that enhancing LAPTM4B function could be a potential therapeutic strategy for alleviating bleomycin-induced lung fibrosis. The findings will be of interest to the lung disease field, and the data presented to support the authors' conclusions is solid. However, it remains unclear whether the suppressive effect of LAPTM4b on idiopathic pulmonary fibrosis is mediated by Nedd4l.

   Reviewed by eLife

7. Phosphodiesterase 1A physically interacts with YTHDF2 and reinforces the progression of non-small cell lung cancer

   This article has 11 authors:

   1. Chong Zhang
   2. Zuoyan Zhang
   3. Yueyi Wu
   4. Yuchen Wu
   5. Jing Cheng
   6. Kaizhi Luo
   7. Zhidi Li
   8. Manman Zhang
   9. Jian Wang
   10. Xuesen Zhang
   11. Yangling Li

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript provides valuable mechanistic insight into NSCLC progression, both in terms of tumour metastasis and the development of chemoresistance. The authors draw upon a range of techniques and assays and the evidence shown is solid and has been strengthened by incorporation of suggestions by the two reviewers. The work presented will be of interest to cancer biologists and more broadly to those interested in NSCLC translational studies.

   Reviewed by eLife

8. HIV integrase compacts viral DNA into biphasic condensates

   This article has 14 authors:

   1. Pauline J Kolbeck
   2. Marjolein de Jager
   3. Margherita Gallano
   4. Tine Brouns
   5. Ben Bekaert
   6. Wout Frederickx
   7. Sebastian F Konrad
   8. Siska Van Belle
   9. Frauke Christ
   10. Steven De Feyter
   11. Zeger Debyser
   12. Laura Filion
   13. Jan Lipfert
   14. Willem Vanderlinden

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The manuscript by Kolbeck and co-workers is an important contribution to understanding the physical mechanism that controls a key step in the retroviral infectious cycle. The authors employ a wide range of experimental techniques, complemented with Montecarlo simulations, that result in convincing evidence of compaction of HIV DNA by the viral integrase. This manuscript would benefit from in-depth discussion and analysis of the biophysical implications of the results.

   Reviewed by eLife

9. Image-based identification and isolation of micronucleated cells to dissect cellular consequences

   This article has 5 authors:

   1. Lucian DiPeso
   2. Sriram Pendyala
   3. Heather Z Huang
   4. Douglas M Fowler
   5. Emily M Hatch

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable paper reports machine learning-based image analysis pipelines for the automated segmentation of micronuclei and the detection and sorting of micronuclei-containing cells. These are powerful new tools for researchers who study micronuclei and their physiologic consequences. The analysis of the new tools and their benchmarking is rigorous and convincing; applications and remaining limitations are well explained in the paper.

   Reviewed by eLife

10. Plectin-mediated cytoskeletal crosstalk as a target for inhibition of hepatocellular carcinoma growth and metastasis

    This article has 31 authors:

    1. Zuzana Outla
    2. Gizem Oyman-Eyrilmez
    3. Katerina Korelova
    4. Magdalena Prechova
    5. Lukas Frick
    6. Lenka Sarnova
    7. Piyush Bisht
    8. Petra Novotna
    9. Jan Kosla
    10. Patricia Bortel
    11. Yasmin Borutzki
    12. Andrea Bileck
    13. Christopher Gerner
    14. Mohammad Rahbari
    15. Nuh Rahbari
    16. Emrullah Birgin
    17. Bibiana Kvasnicova
    18. Andrea Galisova
    19. Katerina Sulkova
    20. Andreas Bauer
    21. Njainday Jobe
    22. Ondrej Tolde
    23. Eva Sticova
    24. Daniel Rösel
    25. Tracy O'Connor
    26. Martin Otahal
    27. Daniel Jirak
    28. Mathias Heikenwälder
    29. Gerhard Wiche
    30. Samuel M Meier-Menches
    31. Martin Gregor

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This valuable study investigated the role of PLECTIN, a cytoskeletal crosslinker protein, in hepatocellular carcinoma development and progression. Using a liver-specific Plectin knockout mouse model, the authors showed solid evidence that PLECTIN is critical for hepatocarcinogenesis, since inhibition of PLECTIN suppressed tumor formation in multiple models. They also show that PLECTIN is key for HCC invasion and metastasis. They show a correlation between PLECTIN inhibition and attenuated FAK, MAPK/ERK, and PI3K/AKT signaling.

    Reviewed by eLife