Molecular Biology

Phenotypic impact of individual conserved neuronal microexons and their master regulators in zebrafish

1. Laura Lopez-Blanch
2. Cristina Rodríguez-Marin
3. Federica Mantica
4. Luis P Iñiguez
5. Jon Permanyer
6. Elizabeth M Kita
7. Tahnee Mackensen
8. Mireia Codina-Tobias
9. Francisco Romero-Ferrero
10. Jordi Fernandez-Albert
11. Myriam Cuadrado
12. Xosé R Bustelo
13. Gonzalo de Polavieja
14. Manuel Irimia

• Curated by eLife

  eLife Assessment

  This important work examines how microexons contribute to brain activity, structure, and behavior. The authors find that loss of microexon sequences generally has subtle impacts on these metrics in larval zebrafish, with few exceptions. The evidence is convincing, using modern high-throughput phenotyping methodology in zebrafish. Overall, this work will be of interest to neuroscientists and generate further studies of interest to the field.

Reviewed by eLife

Brown adipose tissue and skeletal muscle coordinately contribute to thermogenesis in mice

1. Yuna Izumi-Mishima
2. Rie Tsutsumi
3. Tetsuya Shiuchi
4. Saori Fujimoto
5. Momoka Taniguchi
6. Mizuki Sugiuchi
7. Manaka Tsutsumi
8. Yuko Okamatsu-Ogura
9. Takeshi Yoneshiro
10. Masashi Kuroda
11. Kazuhiro Nomura
12. Hiroshi Sakaue

• Curated by eLife

  eLife Assessment

  This is a useful paper regarding the roles of brown adipose tissue and skeletal muscle in thermogenesis in mice, with potential significance for the field. The overall approach is innovative but on balance the evidence for the claim is incomplete, as cast immobilization, while innovative, is likely stressful, may impact muscle and BAT directly, and imposes an energetic cost of motion on the animal that is not accounted for. Further experiments are also needed to directly assess the role of adipose-derived BCAAs in thermogenesis. The authors have done a good job of textually editing their manuscript to clarify the findings and limitations of the study.

Reviewed by eLife

RNA Structure Directs RNA Partitioning and is Actively Disrupted inside Stress Granules to Enable Cellular Recovery

1. Bowen Zhu
2. Jovi Jian An Lim
3. Yu Zhang
4. Wen Ting Tan
5. Jiayi Zhang
6. Joy S Xiang
7. Roland Huber
8. Anthony Khong
9. Yue Wan

Reviewed by preLights

A High-Throughput Multiwell-Plate based Approach for the combined Expression, Export and Assay of Recombinant Proteins

1. Karen Baker
2. Daniel P. Mulvihill

Reviewed by Arcadia Science

MRTF-dependent cytoskeletal dynamics drive efficient cell cycle progression

1. Julie C. Nielsen
2. Maria Benito-Jardon
3. Noel Christo Petrela
4. Jessica Diring
5. Sofie Bellamy
6. Richard Treisman

Reviewed by Review Commons

SETD2 suppresses tumorigenesis in a KRASG12C-driven lung cancer model, and its catalytic activity is regulated by histone acetylation

1. Ricardo J Mack
2. Natasha M Flores
3. Geoffrey C Fox
4. Hanyang Dong
5. Metehan Cebeci
6. Simone Hausmann
7. Tourkian Chasan
8. Jill M Dowen
9. Brian D Strahl
10. Pawel K Mazur
11. Or Gozani

• Curated by eLife

  eLife Assessment

  This is a fundamental study providing molecular insight into how cross-talk between histone modifications regulates the histone H3K36 methyltransferase SETD2. The manuscript contains excellent quality data, and the conclusions are convincing and justified. This work will be of interest to many biochemists working in the field of chromatin biology and epigenetics.

Reviewed by eLife

Magnetic Affinity-Based Purification of His-Tagged Proteins Using Ni ²⁺ -Functionalized Nanoparticles

1. Carolina Otonelo
2. Elisa de Sousa
3. Luciana Juncal
4. Pedro Mendoza Zelis
5. Sheila Ons
6. Claudia Rodríguez Torres
7. Carla Layana

Reviewed by Arcadia Science

Nuclear and cytosolic J-domain proteins provide synergistic control of Hsf1 at distinct phases of the heat shock response

1. Carmen Ruger-Herreros
2. Lucia Svoboda
3. Gurranna Male
4. Aseem Shrivastava
5. Markus Höpfler
6. Katharina Jetzinger
7. Jiří Koubek
8. Günter Kramer
9. Fabian den Brave
10. Axel Mogk
11. David S Gross
12. Bernd Bukau

• Curated by eLife

  eLife Assessment

  This valuable study focuses on defining how the HSP70 chaperone system utilizes J-domain proteins to regulate the heat shock response-associated transcription factor HSF1. Using a combination of orthogonal techniques in yeast, this manuscript provides compelling evidence that the J-domain protein Apj1 facilitates attenuation of HSF1 transcriptional activity through a mechanism involving its dissociation from heat shock gene promoter regions. This work generates new insight into the mechanism of HSF1 transcriptional regulation and is a significant contribution of broad interest to cell biologists interested in proteostasis, chaperone networks, and stress-responsive signaling.

Reviewed by eLife

Introduction of cytosine-5 DNA methylation sensitizes cells to oxidative damage

1. Joanna Krwawicz
2. Caroline J Sheeba
3. Katie Hains
4. Thomas McMahon
5. Yimo Zhang
6. Skirmantas Kriaucionis
7. Peter Sarkies

• Curated by eLife

  eLife Assessment

  This important work advances our understanding of DNA methylation and its consequences for susceptibility to DNA damage. This work presents evidence that DNA methylation can accentuate the genomic damage propagated by DNA damaging agents as well as potentially being an independent source of such damage. The experimental results reported are sound. The evidence presented to support the conclusions drawn is convincing and alternative interpretations are considered. The work will be of broad interest to biochemists, cell and genome biologists.

Reviewed by eLife

Sld3CBD–Cdc45 structural insights into Cdc45 recruitment for CMG complex formation during DNA replication

1. Hao Li
2. Izumi Ishizaki
3. Koji Kato
4. Xiaomei Sun
5. Sachiko Muramatsu
6. Hiroshi Itou
7. Toyoyuki Ose
8. Hiroyuki Araki
9. Min Yao

• Curated by eLife

  eLife Assessment

  This valuable paper describes the crystal structure of a complex of the Sld3-Cdc45-binding domain (CBD) with Cdc45, which is essential for the assembly of an active Cdc45-MCM-GINS (CMG) double-hexamer at the replication origin. The structural and biochemical analyses of protein-protein interactions and DNA binding provided solid evidence to support the authors' conclusion. The results shown in the paper are of interest to researchers in DNA replication and genome stability.

Reviewed by eLife