Molecular Biology

Enhancer cooperativity can compensate for loss of activity over large genomic distances

1. Henry Thomas
2. Songjie Feng
3. Marie Huber
4. Vincent Loubiere
5. Daria Vanina
6. Mattia Pitasi
7. Alexander Stark
8. Christa Buecker

Reviewed by preLights

PAbFold: Linear Antibody Epitope Prediction using AlphaFold2

1. Jacob DeRoo
2. James S. Terry
3. Ning Zhao
4. Timothy J. Stasevich
5. Christopher D. Snow
6. Brian J. Geiss

• Curated by eLife

  eLife assessment

  In this manuscript, the authors describe a new AlphaFold2 pipeline called PabFold that can represent a useful tool for identifying linear antibody epitopes (B-cell epitopes) for different antigens. This information can be used in the selection of different reagents in competitive ELISA assays which can save time and reduce costs. Several questions, however, remain and the study is currently incomplete.

Reviewed by eLife

Newly synthesized mRNA selectively escapes translational repression following acute stress

1. Mostafa Zedan
2. Alexandra P. Schürch
3. Stephanie Heinrich
4. Pablo Aurelio Gómez García
5. Sarah Khawaja
6. Karsten Weis

Reviewed by PREreview

The role of Med15 sequence features in transcription factor interactions

1. David G. Cooper
2. Shulin Liu
3. Emma Grunkemeyer
4. Jan S. Fassler

Reviewed by Review Commons

Recruitment of the m ⁶ A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48

1. Syed Nabeel-Shah
2. Shuye Pu
3. Giovanni L. Burke
4. Nujhat Ahmed
5. Ulrich Braunschweig
6. Shaghayegh Farhangmehr
7. Hyunmin Lee
8. Mingkun Wu
9. Zuyao Ni
10. Hua Tang
11. Guoqing Zhong
12. Edyta Marcon
13. Zhaolei Zhang
14. Benjamin J. Blencowe
15. Jack F. Greenblatt

Reviewed by Review Commons

Co-expression of the AsCas12a ultra variant, a T7 RNA Polymerase and a cytosine base editor greatly increases transfection and editing rates in Leishmania species

1. Nicole Herrmann May
2. Annika Schmid
3. Elisabeth Meiser
4. Tom Beneke

• Curated by eLife

  eLife assessment

  This manuscript describes a method for genetic manipulation of Leishmania species which should be sufficiently efficient to enable genome-wide genetic screens. The authors improved numerous aspects of their previously described method, which is based on sequence-specific genome editing to introduce premature stop codons using a CAS9-cytidine deaminase variant. The work is thoroughly described, with convincing data, and will be very important for Leishmania researchers, as well as perhaps suggesting the use of similar approaches in other organisms in which genetic manipulation is challenging.

Reviewed by eLife

Dissecting translation elongation dynamics through ultra-long tracking of single ribosomes

1. Maximilian F. Madern
2. Sora Yang
3. Olivier Witteveen
4. Marianne Bauer
5. Marvin E. Tanenbaum

Reviewed by PREreview

Eugenol mimics exercise to promote skeletal muscle fiber remodeling and myokine IL-15 expression by activating TRPV1 channel

1. Tengteng Huang
2. Xiaoling Chen
3. Jun He
4. Ping Zheng
5. Yuheng Luo
6. Aimin Wu
7. Hui Yan
8. Bing Yu
9. Daiwen Chen
10. Zhiqing Huang

• Curated by eLife

  eLife assessment

  This useful paper addresses a novel exercise mimetic agent on muscle exercise and performance. While the data provided are interesting, the evidence is incomplete, as much of it is correlative.

Reviewed by eLife

The ASC inflammasome adapter controls the extent of peripheral protein aggregate deposition in inflammation-associated amyloidosis

1. Marco Losa
2. Marc Emmenegger
3. Pierre De Rossi
4. Patrick M Schürch
5. Tetiana Serdiuk
6. Niccolò Pengo
7. Danaëlle Capron
8. Dimitri Bieli
9. Niels J Rupp
10. Manfredi C Carta
11. Karl J Frontzek
12. Veronika Lysenko
13. Regina R Reimann
14. Asvin KK Lakkaraju
15. Mario Nuvolone
16. Gunilla T Westermark
17. K. Peter R. Nilsson
18. Magdalini Polymenidou
19. Alexandre P. A. Theocharides
20. Simone Hornemann
21. Paola Picotti
22. Adriano Aguzzi

Reviewed by Review Commons

Modeling the consequences of age-linked rDNA hypermethylation with dCas9-directed DNA methylation in human cells

1. Yana Blokhina
2. Abigail Buchwalter

Reviewed by Review Commons