Molecular Biology

A TranSNP in the DDIT4 mRNA can impact its translation efficiency and modulate p53-dependent responses in cancer cells

1. Meriem Hadjer Hamadou
2. Laura Alunno
3. Tecla Venturelli
4. Samuel Valentini
5. Davide Dalfovo
6. Francesca Lorenzini
7. Alessia Mattivi
8. Vincenza Vigorito
9. Glenda Paola Grupelli
10. Alessandro Matte’
11. Pamela Gatto
12. Michael Pancher
13. Chiara Valentini
14. Veronica De Sanctis
15. Roberto Bertorelli
16. Virginie Marcel
17. Emilio Cusanelli
18. Stefano Freddi
19. Giovanni Bertalot
20. Sara Zaccara
21. Marina Mione
22. Luca L. Fava
23. Alessandro Romanel
24. Alberto Inga

Reviewed by Review Commons

Single-cell proteomics workflow for characterizing heterogeneous cell populations in saliva and tear fluid

1. Jackson Gabriel Miyamoto
2. Heloísa Monteiro do Amaral-Prado
3. Fábio Malta de Sá Patroni
4. Guilherme Pimentel Telles
5. Carolina Moretto Carnielli
6. Guilherme Araújo Câmara
7. Daniella de Figueiredo
8. Elaine Cristina Cardoso
9. Daniela Campos Granato
10. Alan Roger Santos-Silva
11. Marcio Ajudarte Lopes
12. Adriana Franco Paes Leme

Reviewed by PREreview

Deletion of sulfate transporter SUL1 extends yeast replicative lifespan via reduced PKA signaling instead of decreased sulfate uptake

1. Juan Long
2. Meng Ma
3. Yuting Chen
4. Bo Gong
5. Yi Zheng
6. Hao Li
7. Jing Yang

• Curated by eLife

  eLife Assessment

  This study offers a valuable contribution to the understanding of how inorganic nutrient transporters, particularly SUL1, influence yeast lifespan through signaling pathways rather than transport functions. The findings suggest a novel link between SUL1 deletion and extended replicative lifespan, supported by transcriptomic and stress-response data. However, the strength of the evidence remains incomplete, with key experiments-such as sulfate supplementation tests, functional autophagy validation, and transport assays-either missing or insufficiently described. As a result, while the manuscript presents promising insights, additional work is needed to robustly support its conclusions.

Reviewed by eLife

Sex-lethal is recruited to chromatin to promote neuronal tRNA synthesis in males through RNA Polymerase III regulation

1. Freya Storer
2. Colin D. McClure
3. Alicia Estacio Gomez
4. Tsz Lam Wong
5. Lucy J. Minkley
6. Tony D. Southall

Reviewed by Review Commons

Conserved and unique features of terminal telomeric sequences in ALT-positive cancer cells

1. Benura Azeroglu
2. Wei Wu
3. Raphael Pavani
4. Ranjodh Singh Sandhu
5. Tadahiko Matsumoto
6. André Nussenzweig
7. Eros Lazzerini-Denchi

• Curated by eLife

  eLife Assessment

  This study demonstrates the application of END-seq, originally developed to study genomewide DNA double-strand breaks, to telomere biology; the work packs a punch, concisely demonstrating the utility of this approach and the new insights that can be gained. The authors confirm that telomeres in telomerase-positive cells terminate with 5'-ATC in a Pot1-dependent manner, and demonstrate that this principle holds true in telomerase-negative ALT cells as well. S1-END-seq is similarly developed for telomeres, showing that ALT cells harbor several regions of ssDNA. The study is well-executed and convincing, the new insights are fundamental and compelling, and the optimized END-seq approaches will be widely utilized. The work will prompt additional studies that the reviewers look forward to, including combining telomeric END-seq with long-read sequencing to address the distribution and origin of variant telomere repeats and ssDNA along telomeres in ALT and telomerase-positive settings.

Reviewed by eLife

Structural mechanism of strand exchange by the RAD51 filament

1. Luay Joudeh
2. Robert E Appleby
3. Joseph D Maman
4. Luca Pellegrini

• Curated by eLife

  eLife Assessment

  This landmark study describes the structure of the human RAD51 filament with a recombination intermediate called the displacement loop (D-loop). Using cryogenic structural, biochemical, and single-molecule analyses, the authors provide compelling evidence on how the RAD51 filament promotes strand exchange between single-stranded and double-stranded DNAs. The findings are highly relevant to the fields of homologous recombination, DNA repair, and genome stability.

Reviewed by eLife

Molecular Landscape of the Mouse Adrenal Gland and Adjacent Adipose by Spatial Transcriptomics

1. Małgorzata Blatkiewicz
2. Szymon Hryhorowicz
3. Marta Szyszka
4. Joanna Suszyńska-Zajczyk
5. Andrzej Pławski
6. Adam Plewiński
7. Andrea Porzionato
8. Ludwik K Malendowicz
9. Marcin Rucinski

• Curated by eLife

  eLife Assessment

  This useful study provides a spatial transcriptomic analysis of the mouse adrenal gland that could have implications for future research and applications. The authors present solid results that allow the dissection of the cell signalling pathways and cellular composition of different zones of the adrenal glands in the mouse model; they propose new zone-specific gene markers and specific intra- and inter-zonal signaling pathways based on receptor-ligand expression patterns. Their web tool is user-friendly and will be helpful for adrenal scientists; however, the validation of crucial results of the large dataset is necessary. There are also several contradictory results/interpretations, and the opportunity to dissect the sexually dimorphic gene expression pattern and mouse-human interspecies differences is a missed opportunity.

Reviewed by eLife

Molecular interactome of HNRNPU reveals regulatory networks in neuronal differentiation and DNA methylation

1. Marika Oksanen
2. Francesca Mastropasqua
3. Krystyna Mazan-Mamczarz
4. Jennifer L. Martindale
5. Xuan Ye
6. Abishek Arora
7. Nirad Banskota
8. Myriam Gorospe
9. Kristiina Tammimies

Reviewed by preLights

Boltz-2: Towards Accurate and Efficient Binding Affinity Prediction

1. Saro Passaro
2. Gabriele Corso
3. Jeremy Wohlwend
4. Mateo Reveiz
5. Stephan Thaler
6. Vignesh Ram Somnath
7. Noah Getz
8. Tally Portnoi
9. Julien Roy
10. Hannes Stark
11. David Kwabi-Addo
12. Dominique Beaini
13. Tommi Jaakkola
14. Regina Barzilay

Reviewed by PREreview

Srs2 binding to proliferating cell nuclear antigen (PCNA) and its sumoylation contribute to replication protein A (RPA) antagonism during the DNA damage response

1. Jiayi Fan
2. Nalini Dhingra
3. Tammy Yang
4. Vicki Yang
5. Xiaolan Zhao

• Curated by eLife

  eLife Assessment

  This manuscript reports valuable findings on the role of the Srs2 protein in turning off the DNA damage signaling response initiated by Mec1 (human ATR) kinase. The data provide convincing evidence that Srs2 interaction with PCNA and ensuing SUMO modification is required for checkpoint downregulation. However, while the model that Srs2 acts at gaps after camptothecin-induced DNA damage is reasonable, direct experimental evidence for this is currently lacking. The work will be of interest to cell biologists studying genome integrity.

Reviewed by eLife