Molecular Biology

Robust single nucleus RNA sequencing reveals depot-specific cell population dynamics in adipose tissue remodeling during obesity

1. Jisun So
2. Olivia Strobel
3. Jamie Wann
4. Kyungchan Kim
5. Avishek Paul
6. Dominic J. Acri
7. Luke C. Dabin
8. Gang Peng
9. Jungsu Kim
10. Hyun Cheol Roh

• Curated by eLife

  eLife assessment

  This study employs a modified protocol for single-nuclei RNA sequencing of adipose tissue that preserves RNA quality and nuclei integrity. Using this protocol, the study provides valuable insights into the cellular heterogeneity and molecular landscape of murine adipose tissue from lean mice and mice with diet-induced obesity. The study is solid in its approach and analysis, providing a comprehensive description of a dysfunctional hypertrophic adipocyte subpopulation that emerges in association with obesity.

Reviewed by eLife

Aberrant methylation and expression of TNXB promote chondrocyte apoptosis and extracullar matrix degradation in hemophilic arthropathy via AKT signaling

1. Jiali Chen
2. Qinghe Zeng
3. Xu Wang
4. Rui Xu
5. Weidong Wang
6. Yuliang Huang
7. Qi Sun
8. Wenhua Yuan
9. Pinger Wang
10. Di Chen
11. Peijian Tong
12. Hongting Jin

• Curated by eLife

  eLife assessment

  This important study identifies the TNXB-AKT pathway as a potential mechanism underlying hemophilia-associated cartilage degeneration. The evidence supporting the conclusions is convincing, with murine and human patient evidence as well as genome-wide DNA methylation analysis. This paper would be of interest to cell biologists and biochemists working on the field of musculoskeletal disorders.

Reviewed by eLife

Stomatin-like protein 2 senses oxidative stress through the interaction with phosphatidic acid to promote mitochondrial unfolded protein response

1. Maria Paulina Castelo Rueda
2. Irene Pichler
3. Karolina Musilova
4. Stanislav Kmoch
5. Peter P. Pramstaller
6. Ales Hnizda
7. Andrew A. Hicks
8. Roman Vozdek

Reviewed by Arcadia Science

Lipid-Based Transfection of Zebrafish Embryos: A Robust Protocol for Nucleic Acid Delivery in Zebrafish

1. Aslihan Terzi
2. Tiger Lao
3. Adrian Jacobo

Reviewed by preLights

Late-life dietary folate restriction reduces biosynthetic processes without compromising healthspan in mice

1. Heidi M. Blank
2. Staci E. Hammer
3. Laurel Boatright
4. Courtney Roberts
5. Katarina E. Heyden
6. Aravindh Nagarajan
7. Mitsuhiro Tsuchiya
8. Marcel Brun
9. Charles D. Johnson
10. Patrick J. Stover
11. Raquel Sitcheran
12. Brian K. Kennedy
13. L. Garry Adams
14. Matt Kaeberlein
15. Martha S. Field
16. David W. Threadgill
17. Helene L. Andrews-Polymenis
18. Michael Polymenis

Reviewed by Review Commons

Comparative transcriptomics reveal a novel tardigrade-specific DNA-binding protein induced in response to ionizing radiation

1. Marwan Anoud
2. Emmanuelle Delagoutte
3. Quentin Helleu
4. Alice Brion
5. Evelyne Duvernois-Berthet
6. Marie As
7. Xavier Marques
8. Khadija Lamribet
9. Catherine Senamaud-Beaufort
10. Laurent Jourdren
11. Annie Adrait
12. Sophie Heinrich
13. Geraldine Toutirais
14. Sahima Hamlaoui
15. Giacomo Gropplero
16. Ilaria Giovannini
17. Loic Ponger
18. Marc Geze
19. Corinne Blugeon
20. Yohann Couté
21. Roberto Guidetti
22. Lorena Rebecchi
23. Carine Giovannangeli
24. Anne De Cian
25. Jean-Paul Concordet

• Curated by eLife

  eLife assessment

  This study offers valuable insight into the remarkable resistance of tardigrades to ionizing radiation by showing that radiation treatment induces a suite of DNA repair proteins and by identifying a strongly induced tardigrade-specific DNA-binding protein that can reduce the number of double-strand breaks in human U2OS cells. The evidence of upregulation of repair proteins is convincing, and the case for a role of the newly identified protein in repair can be strengthened as genetic tools for tardigrades become better developed. The results will interest the fields of DNA repair and radiobiology as well as tardigrade biologists.

Reviewed by eLife

Genetic Stability of Mycobacterium smegmatis under the Stress of First-Line Antitubercular Agents: Assessing Mutagenic Potential

1. Dániel Molnár
2. Éva Viola Surányi
3. Tamás Trombitás
4. Dóra Füzesi
5. Rita Hirmondó
6. Judit Tóth

• Curated by eLife

  eLife assessment

  This useful study reports on the impact of antibiotic pressure on the genomic stability of the mc2155 strain of Mycobacterium smegmatis, a model for Mycobacterium tuberculosis. The study concludes that exposure to antibiotics did not lead to the emergence of new adaptive mutations in laboratory settings, contradicting the prevailing theory of antibiotic resistance development through drug-induced microevolution. While the genomic analysis provided detailed insights into the stability of M. smegmatis following exposure to standard TB treatment antibiotics, the evidence presented for antibiotic pressure not contributing to the occurrence of new adaptive mutations is still incomplete.

Reviewed by eLife

Cardiac neurons expressing a glucagon-like receptor mediate cardiac arrhythmia induced by high-fat diet in Drosophila

1. Yunpo Zhao
2. Jianli Duan
3. Joyce van de Leemput
4. Zhe Han

• Curated by eLife

  eLife assessment

  This study reports some useful information on the mechanisms by which a high-fat diet induces arrhythmias in the model organism Drosophila. Specifically, the authors propose that adipokinetic hormone (Akh) secretion is increased with this diet, and through binding of Akh to its receptor on cardiac neurons, arrhythmia is induced. The presented data, however, incompletely support the conclusions, with a number of concerns identified, such as the need for editorial clarifications, issues with experimental design (including additional control experiments), and over or misinterpretation of some of the experimental data. Nonetheless, some of the data will be helpful to those who wish to extend the research to a more complex model system, such as the mouse.

Reviewed by eLife

The PRC2.1 Subcomplex Opposes G1 Progression through Regulation of CCND1 and CCND2

1. Adam D Longhurst
2. Kyle Wang
3. Harsha Garadi Suresh
4. Mythili Ketavarapu
5. Henry N. Ward
6. Ian R. Jones
7. Vivek Narayan
8. Frances V. Hundley
9. Arshia Zernab Hassan
10. Charles Boone
11. Chad Myers
12. Yin Shen
13. Vijay Ramani
14. Brenda J Andrews
15. David P Toczyski

• Curated by eLife

  eLife assessment

  This valuable study reports a chemogenetic screen for resistance and sensitivity towards three compounds that inhibit cell cycle progression: camptothecin, colchicine, and palbociclib. Following up on the palbociclib results, the authors provide solid evidence that knockdown of the PRC2.1 complex, likely through increasing D-type cyclin expression, confers resistance to palbociclib. The generality of the results would be improved by demonstrating the effect of PRC2.1 on cyclin expression and cell cycle progression in more than one cell line.

Reviewed by eLife

Txnip deletions and missense alleles prolong the survival of cones in a retinitis pigmentosa mouse model

1. Yunlu Xue
2. Yimin Zhou
3. Constance L Cepko

• Curated by eLife

  eLife assessment

  This fundamental study advances our understanding of the cell specific treatment of cone photoreceptor degeneration by Txnip. The evidence supporting the conclusions is compelling with rigorous genetic manipulation of Txnip mutations. The work will be of broad interest to vision researchers, cell biologists and biochemists.

Reviewed by eLife