Molecular Biology

A virus‐derived microRNA targets immune response genes during SARS‐CoV‐2 infection

1. Meetali Singh
2. Maxime Chazal
3. Piergiuseppe Quarato
4. Loan Bourdon
5. Christophe Malabat
6. Thomas Vallet
7. Marco Vignuzzi
8. Sylvie van der Werf
9. Sylvie Behillil
10. Flora Donati
11. Nathalie Sauvonnet
12. Giulia Nigro
13. Maryline Bourgine
14. Nolwenn Jouvenet
15. Germano Cecere

Reviewed by PREreview, ScreenIT

Targeting stem-loop 1 of the SARS-CoV-2 5′ UTR to suppress viral translation and Nsp1 evasion

1. Setu M. Vora
2. Pietro Fontana
3. Tianyang Mao
4. Valerie Leger
5. Ying Zhang
6. Tian-Min Fu
7. Judy Lieberman
8. Lee Gehrke
9. Ming Shi
10. Longfei Wang
11. Akiko Iwasaki
12. Hao Wu

Reviewed by ScreenIT

Network analysis outlines strengths and weaknesses of emerging SARS-CoV-2 Spike variants

1. P.D. Manrique
2. S. Chakraborty
3. K. Nguyen
4. R. Mansbach
5. B. Korber
6. S. Gnanakaran

Reviewed by ScreenIT

COVID-19 infection and neurodegeneration: Computational evidence for interactions between the SARS-CoV-2 spike protein and monoamine oxidase enzymes

1. Lucija Hok
2. Hrvoje Rimac
3. Janez Mavri
4. Robert Vianello

Reviewed by ScreenIT

Yeast-expressed recombinant SARS-CoV-2 receptor binding domain RBD203-N1 as a COVID-19 protein vaccine candidate

1. Wen-Hsiang Chen
2. Jeroen Pollet
3. Ulrich Strych
4. Jungsoon Lee
5. Zhuyun Liu
6. Rakhi Tyagi Kundu
7. Leroy Versteeg
8. Maria Jose Villar
9. Rakesh Adhikari
10. Junfei Wei
11. Cristina Poveda
12. Brian Keegan
13. Aaron Oakley Bailey
14. Yi-Lin Chen
15. Portia M. Gillespie
16. Jason T. Kimata
17. Bin Zhan
18. Peter J. Hotez
19. Maria Elena Bottazzi

Reviewed by ScreenIT

Structure-based design of antisense oligonucleotides that inhibit SARS-CoV-2 replication

1. Yan Li
2. Gustavo Garcia
3. Vaithilingaraja Arumugaswami
4. Feng Guo

Reviewed by ScreenIT

End-of-life targeted degradation of DAF-2 insulin/IGF-1 receptor promotes longevity free from growth-related pathologies

1. Richard Venz
2. Tina Pekec
3. Iskra Katic
4. Rafal Ciosk
5. Collin Yvès Ewald

• Curated by eLife

  Evaluation Summary:

  This paper reports careful work using auxin-mediated degradation to manipulate the DAF-2 insulin/IGF-1 receptor in specific tissues and at different ages of the nematode C. elegans. Since its initial discovery as a gene that could dramatically alter lifespan in this organism, daf-2 has been extensively studied. The authors make excellent and thorough use of their novel reagents to successfully add important new findings to our understanding of this broadly conserved aging pathway, including a finer dissection of the spatial and temporal requirements for DAF-2 in multiple processes, such as the decision window for entering dauer arrest and that altering the levels of this protein very late in life can still have dramatic effects on lifespan. Overall the data are convincing, but the presentation and clarity of the text could be improved.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Algorithm for the Quantitation of Variants of Concern for Rationally Designed Vaccines Based on the Isolation of SARS-CoV-2 Hawaiʻi Lineage B.1.243

1. David P. Maison
2. Lauren L. Ching
3. Sean B. Cleveland
4. Alanna C. Tseng
5. Eileen Nakano
6. Cecilia M. Shikuma
7. Vivek R. Nerurkar

Reviewed by ScreenIT

Cap-independent translation and a precisely located RNA sequence enable SARS-CoV-2 to control host translation and escape anti-viral response

1. Boris Slobodin
2. Urmila Sehrawat
3. Anastasia Lev
4. Daniel Hayat
5. Binyamin Zuckerman
6. Davide Fraticelli
7. Ariel Ogran
8. Amir Ben-Shmuel
9. Elad Bar-David
10. Haim Levy
11. Igor Ulitsky
12. Rivka Dikstein

Reviewed by ScreenIT

Ssl2/TFIIH function in transcription start site scanning by RNA polymerase II in Saccharomyces cerevisiae

1. Tingting Zhao
2. Irina O Vvedenskaya
3. William KM Lai
4. Shrabani Basu
5. B Franklin Pugh
6. Bryce E Nickels
7. Craig D Kaplan

• Curated by eLife

  Evaluation Summary:

  Kaplan and colleagues build upon their earlier work using genetic phenotypes to find and analyze mutations that determine how mRNA start sites are chosen. Here they provide convincing genetic evidence supporting a model in which the Transcription Factor II H (TFIIH) protein complex pushes downstream DNA back into the RNA polymerase active site, creating a window within which the polymerase can choose particular start sites. This will primarily interest those in the transcription field who are thinking about initiation mechanisms.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife