Molecular Biology

ACE2 pathway regulates thermogenesis and energy metabolism

1. Xi Cao
2. Ting-Ting Shi
3. Chuan-Hai Zhang
4. Wan-Zhu Jin
5. Li-Ni Song
6. Yi-Chen Zhang
7. Jing-Yi Liu
8. Fang-Yuan Yang
9. Charles N Rotimi
10. Aimin Xu
11. Jin-Kui Yang

• Curated by eLife

  Evaluation Summary:

  With a series of elegant experiments, the authors have shown that the renin-angiotensin system (RAS) plays a critical role in thermogenesis. Involvement of brown fat and mitochondrial chain open new scenarios that may be helpful to define new target pathways for the treatment of obesity and diabetes.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Inhibition of SARS-CoV-2 spike protein palmitoylation reduces virus infectivity

1. Ahmed A. Ramadan
2. Karthick Mayilsamy
3. Andrew R. McGill
4. Anandita Ghosh
5. Marc A. Giulianotti
6. Haley M. Donow
7. Shyam S. Mohapatra
8. Subhra Mohapatra
9. Bala Chandran
10. Robert J. Deschenes
11. Arunava Roy

Reviewed by ScreenIT

An entropic safety catch controls hepatitis C virus entry and antibody resistance

1. Lenka Stejskal
2. Mphatso D Kalemera
3. Charlotte B Lewis
4. Machaela Palor
5. Lucas Walker
6. Tina Daviter
7. William D Lees
8. David S Moss
9. Myrto Kremyda-Vlachou
10. Zisis Kozlakidis
11. Giulia Gallo
12. Dalan Bailey
13. William Rosenberg
14. Christopher JR Illingworth
15. Adrian J Shepherd
16. Joe Grove

• Curated by eLife

  Evaluation Summary:

  HCV is unique in its glycoprotein structure, complex receptor usage and its unusual persistence for a (+)RNA virus. This is a well done study that explains a number of observations regarding receptor usage and how HCV may evade antibody control via HVR1 due to its disordered nature, enable mutation to continually evade antibody responses. This manuscript should be of substantial interest to those in the fields of virus entry, vaccination against human viruses, and the study of how intrinsically disordered regions can play regulatory roles.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

SARS- CoV-2 viroporins: A multi-omics insight from nucleotides to amino acids

1. Manish Sarkar
2. Paul Etheimer
3. Victor Hannothiaux
4. Soham Saha

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Analysis of the upper respiratory tract microbiota in mild and severe COVID-19 patients

1. V. Babenko
2. R. Bakhtyev
3. V. Baklaushev
4. L. Balykova
5. P. Bashkirov
6. J. Bespyatykh
7. A. Blagonravova
8. D. Boldyreva
9. D. Fedorov
10. I. Gafurov
11. R. Gaifullina
12. J. Galeeva
13. E. Galova
14. A. Gospodaryk
15. E. Ilina
16. K. Ivanov
17. D. Kharlampieva
18. P. Khromova
19. K. Klimina
20. K. Kolontarev
21. N. Kolyshkina
22. A. Koritsky
23. V. Kuropatkin
24. V. Lazarev
25. A. Manolov
26. V. Manuvera
27. D. Matyushkina
28. M. Morozov
29. E. Moskaleva
30. V. Musarova
31. O. Ogarkov
32. E. Orlova
33. A. Pavlenko
34. A. Petrova
35. N. Pozhenko
36. D. Pushkar
37. A. Rumyantsev
38. S. Rumyantsev
39. V. Rumyantsev
40. L. Rychkova
41. A. Samoilov
42. I. Shirokova
43. V. Sinkov
44. S. Solovieva
45. E. Starikova
46. P. Tikhonova
47. G. Trifonova
48. A. Troitsky
49. A. Tulichev
50. Y. Udalov
51. A. Varizhuk
52. A. Vasiliev
53. R. Vereshchagin
54. V. Veselovsky
55. A. Volnukhin
56. G. Yusubalieva
57. V. Govorun

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A BioID-Derived Proximity Interactome for SARS-CoV-2 Proteins

1. Danielle G. May
2. Laura Martin-Sancho
3. Valesca Anschau
4. Sophie Liu
5. Rachel J. Chrisopulos
6. Kelsey L. Scott
7. Charles T. Halfmann
8. Ramon Díaz Peña
9. Dexter Pratt
10. Alexandre R. Campos
11. Kyle J. Roux

Reviewed by ScreenIT

Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells

1. Sarah G Swygert
2. Dejun Lin
3. Stephanie Portillo-Ledesma
4. Po-Yen Lin
5. Dakota R Hunt
6. Cheng-Fu Kao
7. Tamar Schlick
8. William S Noble
9. Toshio Tsukiyama

• Curated by eLife

  Evaluation Summary:

  This work characterizes chromatin compaction in quiescent yeast cells and its role in the repression of gene expression. The authors' findings that chromatin compaction via heterogeneous interactions between nucleosomes directly contributes to transcriptional repression provides a useful conceptual paradigm for studies of quiescence in other organisms.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Principles of mRNA targeting via the Arabidopsis m6A-binding protein ECT2

1. Laura Arribas-Hernández
2. Sarah Rennie
3. Tino Köster
4. Carlotta Porcelli
5. Martin Lewinski
6. Dorothee Staiger
7. Robin Andersson
8. Peter Brodersen

• Curated by eLife

  Evaluation Summary:

  Gene regulation triggered by recognising N6-methyladenosine (m6A) by RNA-binding proteins emerged as a fundamental process for development and adaptation in most eukaryotes. This paper is of interest to scientists within the field of post-transcriptional gene regulation and RNA biology. In a deep and thorough analysis, the authors resolved previously contradictory reports of sequence motifs associated with methylation sites in messenger RNAs and shed light on the proteins that recognise this modification.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

  This manuscript was co-submitted with: https://www.biorxiv.org/content/10.1101/2021.08.01.454660v1

Reviewed by eLife

The YTHDF proteins ECT2 and ECT3 bind largely overlapping target sets and influence target mRNA abundance, not alternative polyadenylation

1. Laura Arribas-Hernández
2. Sarah Rennie
3. Michael Schon
4. Carlotta Porcelli
5. Balaji Enugutti
6. Robin Andersson
7. Michael D Nodine
8. Peter Brodersen

• Curated by eLife

  Evaluation Summary:

  Post-transcriptional gene regulation mediated by YTH proteins that bind N6-methyladenosine (m6A) in mRNA is a pathway that has emerged of importance in eukaryotic biology. This paper focuses on two evolutionarily close proteins of this family, ETC2 and ETC3 to find redundant, specific and divergent functions. The authors addressed previously contradictory reports regarding the subcellular location of these proteins. The paper is of general interest to scientists within the field of post-transcriptional gene regulation and RNA biology and is not limited to the plant research community. This will advance our understanding of mRNA methylation and its role in plant biology.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

  This manuscript was co-submitted with: https://www.biorxiv.org/content/10.1101/2021.04.18.440342v2

Reviewed by eLife

The key features of SARS-CoV-2 leader and NSP1 required for viral escape of NSP1-mediated repression

1. Lucija Bujanic
2. Olga Shevchuk
3. Nicolai von Kügelgen
4. Anna Kalinina
5. Katarzyna Ludwik
6. David Koppstein
7. Nadja Zerna
8. Albert Sickmann
9. Marina Chekulaeva

Reviewed by ScreenIT