Molecular Biology

Rif2 protects Rap1-depleted telomeres from MRX-mediated degradation in Saccharomyces cerevisiae

1. Fernando Rodrigo Rosas Bringas
2. Sonia Stinus
3. Pien de Zoeten
4. Marita Cohn
5. Michael Chang

• Curated by eLife

  Evaluation Summary:

  This study investigates the tolerance to change in the sequence of telomere repeats, by analyzing a strain expressing mutant TLC1, the RNA component of the telomerase. The authors conclude that Rif2 protects telomere ends in the absence of RAP1 by inhibiting MRX and promoting Rad51-dependent homologous recombination to maintain telomere homeostasis. The study clarifies the role of Rif2 in telomere homeostais and describes how cells can extend telomeres and control senescence in the absence of Rap1 binding to telomeres. The possibility of coping with telomere sequence modification through flexibility and redundancy of capping proteins is of general interest in terms of telomere evolution.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Exploring functional protein covariation across single cells using nPOP

1. Andrew Leduc
2. R. Gray Huffman
3. Joshua Cantlon
4. Saad Khan
5. Nikolai Slavov

Reviewed by ASAPbio crowd review

A rapid bead-based assay for screening of SARS-CoV-2 neutralizing antibodies

1. Santhik Subhasingh Lupitha
2. Pramod Darvin
3. Aneesh Chandrasekharan
4. Shankara Narayanan Varadarajan
5. Soumya Jaya Divakaran
6. Sreekumar Easwaran
7. Shijulal Nelson-Sathi
8. Perunthottathu K Umasankar
9. Sara Jones
10. Iype Joseph
11. Madhavan Radhakrishna Pillai
12. Thankayyan Retnabai Santhoshkumar

Reviewed by ScreenIT

SARS-CoV-2 infects and replicates in photoreceptor and retinal ganglion cells of human retinal organoids

1. Yotam Menuchin-Lasowski
2. André Schreiber
3. Aarón Lecanda
4. Angeles Mecate-Zambrano
5. Linda Brunotte
6. Olympia E. Psathaki
7. Stephan Ludwig
8. Thomas Rauen
9. Hans R. Schöler

Reviewed by ScreenIT

Secondary Structure of Subgenomic RNA M of SARS-CoV-2

1. Marta Soszynska-Jozwiak
2. Agnieszka Ruszkowska
3. Ryszard Kierzek
4. Collin A. O’Leary
5. Walter N. Moss
6. Elzbieta Kierzek

Reviewed by ScreenIT

Highly resolved spatial transcriptomics for detection of rare events in cells

1. Silvia Groiss
2. Daniela Pabst
3. Cynthia Faber
4. Andreas Meier
5. Annette Bogdoll
6. Conny Unger
7. Benedikt Nilges
8. Sascha Strauss
9. Esther Föderl-Höbenreich
10. Melina Hardt
11. Andreas Geipel
12. Frank Reinecke
13. Christian Korfhage
14. Kurt Zatloukal

Reviewed by ScreenIT

A bacteria-based assay to study SARS-CoV-2 protein-protein interactions

1. Benjamin L. Springstein
2. Padraig Deighan
3. Grzegorz Grabe
4. Ann Hochschild

Reviewed by ScreenIT

ACE2 pathway regulates thermogenesis and energy metabolism

1. Xi Cao
2. Ting-Ting Shi
3. Chuan-Hai Zhang
4. Wan-Zhu Jin
5. Li-Ni Song
6. Yi-Chen Zhang
7. Jing-Yi Liu
8. Fang-Yuan Yang
9. Charles N Rotimi
10. Aimin Xu
11. Jin-Kui Yang

• Curated by eLife

  Evaluation Summary:

  With a series of elegant experiments, the authors have shown that the renin-angiotensin system (RAS) plays a critical role in thermogenesis. Involvement of brown fat and mitochondrial chain open new scenarios that may be helpful to define new target pathways for the treatment of obesity and diabetes.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Inhibition of SARS-CoV-2 spike protein palmitoylation reduces virus infectivity

1. Ahmed A. Ramadan
2. Karthick Mayilsamy
3. Andrew R. McGill
4. Anandita Ghosh
5. Marc A. Giulianotti
6. Haley M. Donow
7. Shyam S. Mohapatra
8. Subhra Mohapatra
9. Bala Chandran
10. Robert J. Deschenes
11. Arunava Roy

Reviewed by ScreenIT

An entropic safety catch controls hepatitis C virus entry and antibody resistance

1. Lenka Stejskal
2. Mphatso D Kalemera
3. Charlotte B Lewis
4. Machaela Palor
5. Lucas Walker
6. Tina Daviter
7. William D Lees
8. David S Moss
9. Myrto Kremyda-Vlachou
10. Zisis Kozlakidis
11. Giulia Gallo
12. Dalan Bailey
13. William Rosenberg
14. Christopher JR Illingworth
15. Adrian J Shepherd
16. Joe Grove

• Curated by eLife

  Evaluation Summary:

  HCV is unique in its glycoprotein structure, complex receptor usage and its unusual persistence for a (+)RNA virus. This is a well done study that explains a number of observations regarding receptor usage and how HCV may evade antibody control via HVR1 due to its disordered nature, enable mutation to continually evade antibody responses. This manuscript should be of substantial interest to those in the fields of virus entry, vaccination against human viruses, and the study of how intrinsically disordered regions can play regulatory roles.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife