Microbiology

A pH-sensitive switch activates virulence in Salmonella

1. Dasvit Shetty
2. Linda J Kenney

• Curated by eLife

  eLife assessment

  Salmonella invades and survives in host cells via SPI-1 and SPI-2 type III secretion system mechanisms, with the SPI-2 system allowing for intracellular survival in Salmonella-containing vacuoles, which have a low-pH environment. Transcription of SPI-2 genes at low pH is activated by the DNA-binding SsrB protein, which sits at the top of the SPI-2 regulatory hierarchy. This study provides important insights as to how SsrB is allosterically affected by pH resulting in acid-dependent DNA binding. However, there are concerns about some experiments, and the evidence presented is not fully conclusive.

Reviewed by eLife

ORBIT for E. coli : Kilobase-scale oligonucleotide recombineering at high throughput and high efficiency

1. Scott H. Saunders
2. Ayesha M. Ahmed

Reviewed by Arcadia Science

Novel role for peptidoglycan carboxypeptidases in maintaining the balance between bacterial cell wall synthesis and degradation

1. Manuela Alvarado Obando
2. Tobias Dörr

Reviewed by Arcadia Science

Replaying the tape of ecology to domesticate wild microbiota

1. Alberto Pascual-García
2. Damian Rivett
3. Matt L. Jones
4. Thomas Bell

Reviewed by Arcadia Science

Decomposing a San Francisco Estuary microbiome using long read metagenomics reveals species and species- and strain-level dominance from picoeukaryotes to viruses

1. Lauren M. Lui
2. Torben N. Nielsen

Reviewed by Arcadia Science

A Highly Immunogenic Measles Virus-based Th1-biased COVID-19 Vaccine

1. Cindy Hörner
2. Christoph Schürmann
3. Arne Auste
4. Aileen Ebenig
5. Samada Muraleedharan
6. Maike Herrmann
7. Barbara Schnierle
8. Michael D. Mühlebach

Reviewed by ScreenIT

Human angiotensin-converting enzyme 2 transgenic mice infected with SARS-CoV-2 develop severe and fatal respiratory disease

1. Joseph W. Golden
2. Curtis R. Cline
3. Xiankun Zeng
4. Aura R. Garrison
5. Brian D. Carey
6. Eric M. Mucker
7. Lauren E. White
8. Joshua D. Shamblin
9. Rebecca L. Brocato
10. Jun Liu
11. April M. Babka
12. Hypaitia B. Rauch
13. Jeffrey M. Smith
14. Bradley S. Hollidge
15. Collin Fitzpatrick
16. Catherine V. Badger
17. Jay W. Hooper

Reviewed by ScreenIT

A single-dose live-attenuated YF17D-vectored SARS-CoV2 vaccine candidate

1. Lorena Sanchez Felipe
2. Thomas Vercruysse
3. Sapna Sharma
4. Ji Ma
5. Viktor Lemmens
6. Dominique van Looveren
7. Mahadesh Prasad Arkalagud Javarappa
8. Robbert Boudewijns
9. Bert Malengier-Devlies
10. Suzanne F. Kaptein
11. Laurens Liesenborghs
12. Carolien De Keyzer
13. Lindsey Bervoets
14. Madina Rasulova
15. Laura Seldeslachts
16. Sander Jansen
17. Michael Bright Yakass
18. Osbourne Quaye
19. Li-Hsin Li
20. Xin Zhang
21. Sebastiaan ter Horst
22. Niraj Mishra
23. Lotte Coelmont
24. Christopher Cawthorne
25. Koen Van Laere
26. Ghislain Opdenakker
27. Greetje Van de Velde
28. Birgit Weynand
29. Dirk E. Teuwen
30. Patrick Matthys
31. Johan Neyts
32. Hendrik Jan Thibaut
33. Kai Dallmeier

Reviewed by ScreenIT

Continuous flexibility analysis of SARS-CoV-2 spike prefusion structures

1. Roberto Melero
2. Carlos Oscar S. Sorzano
3. Brent Foster
4. José-Luis Vilas
5. Marta Martínez
6. Roberto Marabini
7. Erney Ramírez-Aportela
8. Ruben Sanchez-Garcia
9. David Herreros
10. Laura del Caño
11. Patricia Losana
12. Yunior C. Fonseca-Reyna
13. Pablo Conesa
14. Daniel Wrapp
15. Pablo Chacon
16. Jason S. McLellan
17. Hemant D. Tagare
18. Jose-Maria Carazo

Reviewed by ScreenIT

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs

1. Lei Sun
2. Pan Li
3. Xiaohui Ju
4. Jian Rao
5. Wenze Huang
6. Lili Ren
7. Shaojun Zhang
8. Tuanlin Xiong
9. Kui Xu
10. Xiaolin Zhou
11. Mingli Gong
12. Eric Miska
13. Qiang Ding
14. Jianwei Wang
15. Qiangfeng Cliff Zhang

Reviewed by ScreenIT