Microbiology

Structure of the two-component S-layer of the archaeon Sulfolobus acidocaldarius

1. Lavinia Gambelli
2. Mathew McLaren
3. Rebecca Conners
4. Kelly Sanders
5. Matthew C Gaines
6. Lewis Clark
7. Vicki AM Gold
8. Daniel Kattnig
9. Mateusz Sikora
10. Cyril Hanus
11. Michail N Isupov
12. Bertram Daum

• Curated by eLife

  eLife assessment

  This fundamental work substantially advances our structural understanding of S-layers in Archaea and how they are built to form formidable cell support structures able to stabilise the cytoplasmic membrane under harsh physicochemical conditions. The supporting evidence for the S-layer model is convincing, making excellent use of state-of-the-art 3D cryo-electron tomography reconstructions, although the proposed S-layer model would benefit from some additional validation.

Reviewed by eLife

Salmonella succinate utilisation is inhibited by multiple regulatory systems

1. Nicolas Wenner
2. Xiaojun Zhu
3. Will P. M. Rowe
4. Kristian Händler
5. Jay C. D. Hinton

Reviewed by Review Commons

Plasma activated water as a pre-treatment strategy in the context of biofilm-infected chronic wounds

1. Heema K.N. Vyas
2. Binbin Xia
3. David Alam
4. Nicholas P. Gracie
5. Joanna G. Rothwell
6. Scott A. Rice
7. Dee Carter
8. Patrick J. Cullen
9. Anne Mai-Prochnow

Reviewed by PREreview

Cell arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms

1. Hannah Dayton
2. Julie Kiss
3. Mian Wei
4. Shradha Chauhan
5. Emily LaMarre
6. William Cole Cornell
7. Chase J. Morgan
8. Anuradha Janakiraman
9. Wei Min
10. Raju Tomer
11. Alexa Price-Whelan
12. Jasmine A Nirody
13. Lars E.P. Dietrich

Reviewed by PREreview

SIRT-1 is required for release of enveloped enteroviruses

1. Alagie Jassey
2. James Logue
3. Stuart Weston
4. Michael A Wagner
5. Ganna Galitska
6. Katelyn Miller
7. Matthew Frieman
8. William T Jackson

• Curated by eLife

  eLife assessment

  The presence or absence of a surrounding envelope, previously a clear distinguishing feature of different viruses, has been blurred by the recent recognition that many so-called 'nonenveloped' viruses are released from cells as quasi-enveloped virions cloaked in host cell membranes. This mechanism of viral egress allows for non-lytic infection, and has potentially important implications for pathogenesis. In this manuscript, Jassey and colleagues provide solid evidence that the protein deacetylase SIRT-1 is required for the non-lytic release of enteroviruses in extracellular vesicles.

Reviewed by eLife

σ28-dependent small RNA regulation of flagella biosynthesis

1. Sahar Melamed
2. Aixia Zhang
3. Michal Jarnik
4. Joshua Mills
5. Aviezer Silverman
6. Hongen Zhang
7. Gisela Storz

• Curated by eLife

  eLife assessment

  This article provides important findings on how bacteria use small RNAs to regulate flagellar expression with implications for multiple fields. The data supporting the conclusions are convincing with a large amount of data that include results from phenotypic analyses, genomics approaches as well as in-vitro and in-vivo target identification and validation methods. This study on the varied effects of three sRNAs (UhpU, FliX and MotR) is of broad interest to RNA biochemists and microbiologists.

Reviewed by eLife

A phage nucleus-associated RNA-binding protein is required for jumbo phage infection

1. Eray Enustun
2. Emily G Armbruster
3. Jina Lee
4. Sitao Zhang
5. Brian A Yee
6. Kseniya Malukhina
7. Yajie Gu
8. Amar Deep
9. Jack T Naritomi
10. Qishan Liang
11. Stefan Aigner
12. Benjamin A Adler
13. Brady F Cress
14. Jennifer A Doudna
15. Vorrapon Chaikeeratisak
16. Don W Cleveland
17. Majid Ghassemian
18. Bogdan Bintu
19. Gene W Yeo
20. Joe Pogliano
21. Kevin D Corbett

Reviewed by Arcadia Science

Persistence of intact HIV-1 proviruses in the brain during antiretroviral therapy

1. Weiwei Sun
2. Yelizaveta Rassadkina
3. Ce Gao
4. Sarah Isabel Collens
5. Xiaodong Lian
6. Isaac H Solomon
7. Shibani S Mukerji
8. Xu G Yu
9. Mathias Lichterfeld

• Curated by eLife

  eLife assessment

  This important study uses near full-length HIV-1 sequencing to examine proviral persistence in various tissues derived from three individuals who received antiretroviral therapy until time of death. Intact as well as defective HIV-1 proviruses are found at various anatomical sites including the central nervous system; the results are convincing and relevant for our understanding of latent viral reservoirs, especially in the brain.

Reviewed by eLife

Chikungunya virus glycoproteins transform macrophages into productive viral dissemination vessels

1. Zhenlan Yao
2. Sangeetha Ramachandran
3. Serina Huang
4. Yasaman Jami-Alahmadi
5. James A. Wohlschlegel
6. Melody M.H. Li

Reviewed by Review Commons

C. difficile may be overdiagnosed in adults and is a prevalent commensal in infants

1. Pamela Ferretti
2. Jakob Wirbel
3. Oleksandr M Maistrenko
4. Thea Van Rossum
5. Renato Alves
6. Anthony Fullam
7. Wasiu Akanni
8. Christian Schudoma
9. Anna Schwarz
10. Roman Thielemann
11. Leonie Thomas
12. Stefanie Kandels
13. Rajna Hercog
14. Anja Telzerow
15. Ivica Letunic
16. Michael Kuhn
17. Georg Zeller
18. Thomas SB Schmidt
19. Peer Bork

• Curated by eLife

  eLife assessment

  This important study points out discrepancies between the clinical diagnosis of Clostridioides difficile infection and the lack of detectable C. difficile in gut microbiome samples, as well as different relationships between asymptomatic C. difficile carriage and adult or infant gut microbiota compositions. While the solid analysis of a comprehensive and diverse metagenomic dataset suggests an over-diagnosis of C. difficile infection and an under-diagnosis of other putative enteric pathogens, the work requires addressing the detection limitations of the approach to be more convincing. This work will interest microbiologists and clinicians concerned with understanding the role of C. difficile in gut microbiota health and dysbiosis.

Reviewed by eLife