Microbiology

Temperature modulates the dissemination potential of Microsporidia MB, a malaria-blocking endosymbiont of Anopheles mosquitoes

1. Fidel Gabriel Otieno
2. Priscille Barreaux
3. Affognon Steeven Belvinos
4. Edward Edmond Makhulu
5. Thomas Ogao Onchuru
6. Anne Wambui Wairimu
7. Stancy Mandere Omboye
8. Cynthia Nyambura King’ori
9. Sokame Bonoukpoè Mawuko
10. Anthony Nyamache Kebira
11. Jeremy Keith Herren

Reviewed by Rapid Reviews Infectious Diseases

The influence of the pre-membrane and envelope proteins on structure, pathogenicity and tropism of tick-borne encephalitis virus

1. Ebba Rosendal
2. Kyrylo Bisikalo
3. Stefanie M.A. Willekens
4. Marie Lindgren
5. Jiří Holoubek
6. Pavel Svoboda
7. Amanda Lappalainen
8. Ebba Könighofer
9. Ekaterina Mirgorodskaya
10. Rickard Nordén
11. Federico Morini
12. William Rosenbaum
13. Daniel Růžek
14. Ulf Ahlgren
15. Maria Anastasina
16. Andres Merits
17. Sarah J Butcher
18. Emma Nilsson
19. Anna K Överby

Reviewed by Review Commons

SATAY-based chemogenomic screening uncovers antifungal resistance mechanisms and key determinants of ATI-2307 and chitosan sensitivity

1. Matthew T Karadzas
2. Agnès H Michel
3. Andreas Mosbach
4. George Giannakopoulos
5. Ruairi McGettigan
6. Gabriel Scalliet
7. Benoît Kornmann

• Curated by eLife

  eLife Assessment

  This important manuscript proposes a new strategy for the identification of new mechanisms of drug resistance based on SAturated Transposon Analysis in Yeast (SATAY), a powerful transposon sequencing method in Saccharomyces cerevisiae. This method allows us to uncover loss- and gain-of-function mutations conferring resistance to 20 different antifungal compounds. The method is convincing, allowing the authors to identify a novel interaction of chitosan with the cell wall mannosylphosphate, and show that the transporter Hol1 concentrates the novel antifungal ATI-2307 within yeast.

Reviewed by eLife