The bacterial microbiome of symbiotic and menthol-bleached polyps of long-term aquarium-reared Galaxea fascicularis

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  1. Despite their high abundance and prevalence in this study (and in corals in general), the role of Alteromonas spp. remains controversial.

    Maybe different but closely related species have different roles?

  2. three polyps per colony (5 colonies: RS1, RS2, RS3, HK1, HK2) per state (2 states: symbiotic, bleached) were sampled on the 13th day after the menthol treatment when they visually appeared completely bleached and otherwise healthy (n = 15 bleached and 15 symbiotic polyps)

    How did you select this time point for sampling? Do you expect the bacterial population composition would change after this point presumably due to some bacterial species benefiting from the presence of Symbiodinium? A number of time points could be informative.

  3. Figure 4.

    It would be interesting to see if the bacterial species that are abundant in the Red Sea type 1 but missing in the other types fill the same niche as the species that are abundant in the Hong Kong type but missing in the Red Sea type 1 (eg Moraxellaceae). Maybe metabolomics would be a cool way of looking at this?