Microbiology

Amoxicillin-resistant Streptococcus pneumoniae can be resensitized by targeting the mevalonate pathway as indicated by sCRilecs-seq

1. Liselot Dewachter
2. Julien Dénéréaz
3. Xue Liu
4. Vincent de Bakker
5. Charlotte Costa
6. Mara Baldry
7. Jean-Claude Sirard
8. Jan-Willem Veening

• Curated by eLife

  Evaluation Summary:

  Three experts in the field reviewed this manuscript from slightly different perspectives. All three reviewers are generally positive about this interesting, well-presented paper and think that it leads to several advances in the field. However, the reviewers also think changes can be made that would considerably strengthen the current version and its impact. Specific modifications have been requested to improve analysis of the screening data, to discuss hits besides the mevalonate pathway that increase Streptococcus pneumoniae cell length and shape, to clarify some issues about how mevalonate depletion changes pneumococcal cell shape and peptidoglycan synthesis, and to provide more context for clomiphene potentiation of amoxicillin killing of Streptococcus pneumoniae in comparison to previously published results in Staphylococcus aureus.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Geneticin shows selective antiviral activity against SARS-CoV-2 by interfering with programmed −1 ribosomal frameshifting

1. Carmine Varricchio
2. Gregory Mathez
3. Trestan Pillonel
4. Claire Bertelli
5. Laurent Kaiser
6. Caroline Tapparel
7. Andrea Brancale
8. Valeria Cagno

Reviewed by ScreenIT

SARS-CoV-2 Spike evolution influences GBP and IFITM sensitivity

1. Dejan Mesner
2. Ann-Kathrin Reuschl
3. Matthew V.X Whelan
4. Taylor Bronzovich
5. Tafhima Haider
6. Lucy G. Thorne
7. Greg J. Towers
8. Clare Jolly

Reviewed by ScreenIT

Crippled Coronavirus: 5’-PolyU targeted Oligo prevents development of infectious Virions

1. Hemayet Ullah
2. Saadyah Averick
3. Qiyi Tang

Reviewed by ScreenIT

A mechanistic understanding of the modes of Ca ion binding to the SARS-CoV-1 fusion peptide and their role in the dynamics of host membrane penetration

1. Juliana Debrito Carten
2. George Khelashvili
3. Miya K. Bidon
4. Marco R. Straus
5. Tiffany Tang
6. Javier A. Jaimes
7. Harel Weinstein
8. Gary R. Whittaker
9. Susan Daniel

Reviewed by ScreenIT

Microevolution of Trypanosoma cruzi reveals hybridization and clonal mechanisms driving rapid genome diversification

1. Gabriel Machado Matos
2. Michael D Lewis
3. Carlos Talavera-López
4. Matthew Yeo
5. Edmundo C Grisard
6. Louisa A Messenger
7. Michael A Miles
8. Björn Andersson

• Curated by eLife

  Evaluation Summary:

  In this paper the authors dissect the across-the-genome consequences of sexual recombination in Trypanosoma cruzi. It summarises an extensive piece of work, which includes a 5-year in vitro growing of parasites and relatively rigorous genome analyses. It will be of interest to microbiologists working on microbes with cryptic or parasexual modes of genetic exchange.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Impact of a human gut microbe on Vibrio cholerae host colonization through biofilm enhancement

1. Kelsey Barrasso
2. Denise Chac
3. Meti D Debela
4. Catherine Geigel
5. Anjali Steenhaut
6. Abigail Rivera Seda
7. Chelsea N Dunmire
8. Jason B Harris
9. Regina C Larocque
10. Firas S Midani
11. Firdausi Qadri
12. Jing Yan
13. Ana A Weil
14. Wai-Leung Ng

• Curated by eLife

  Evaluation Summary:

  In this work, the authors study the previously reported positive association between the presence of the gut bacterium Paracoccus aminovorans and Vibrio cholerae during infection. They describe and image dual species-biofilm formed in vitro as well as enhanced V. cholerae gut colonization in the presence of P. aminovorans in a neonatal mouse model. Collectively, the authors conclude that P. aminovorans enhances biofilm formation by Vc, which could explain the increased susceptibility of P. aminovorans-containing humans in cholera endemic areas.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Determining the Optimal SARS-CoV-2 mRNA Vaccine Dosing Interval for Maximum Immunogenicity

1. Michael Asamoah-Boaheng
2. David Goldfarb
3. Martin A Prusinkiewicz
4. Liam Golding
5. Mohammad E Karim
6. Vilte Barakauskas
7. Nechelle Wall
8. Agatha N Jassem
9. Ana Citlali Marquez
10. Chris MacDonald
11. Sheila F O’Brien
12. Pascal Lavoie
13. Brian Grunau

Reviewed by ScreenIT

Z-RNA and the flipside of the SARS Nsp13 helicase

1. Alan Herbert
2. Maria Poptsova

Reviewed by ScreenIT

Comparative infection and pathogenesis of SARS-CoV-2 Omicron and Delta variants in aged and young Syrian hamsters

1. Nadia Storm
2. Nicholas A. Crossland
3. Lindsay G. A. McKay
4. Anthony Griffiths

Reviewed by ScreenIT