A rapid, high-throughput, viral infectivity assay using automated brightfield microscopy with machine learning

Article activity feed

1. Version published to 10.1016/j.slast.2023.07.003

   Oct 1, 2023
2. 

   ScreenIT

   Mar 30, 2022

   SciScore for 10.1101/2022.03.23.485512: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   NIH rigor criteria are not applicable to paper type.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   The cultures for Corona 229E-infected Huh7 cells and Influenza A-infected MDCK cells were prepared as described in et al.51, and Crisci et al.52, respectively.	Huh7 suggested: CLS Cat# 300156/p7178_HuH7, RRID:CVCL_0336)
   Adenovirus-infected HeLa cells were prepared as described in Jogler et al.56, vaccinia-infected BHK-21 cells were prepared as described in Earl et al.57, polio-infected HeLa cells were prepared as described in et al.58.	HeLa suggested: None BHK-21 suggested: ATCC Cat# CRL-6281, RRID:CVCL_1914)
   Influenza-infected MDCK cells (see Supplementary Fig. 9) were prepared as …

   SciScore for 10.1101/2022.03.23.485512: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   NIH rigor criteria are not applicable to paper type.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   The cultures for Corona 229E-infected Huh7 cells and Influenza A-infected MDCK cells were prepared as described in et al.51, and Crisci et al.52, respectively.	Huh7 suggested: CLS Cat# 300156/p7178_HuH7, RRID:CVCL_0336)
   Adenovirus-infected HeLa cells were prepared as described in Jogler et al.56, vaccinia-infected BHK-21 cells were prepared as described in Earl et al.57, polio-infected HeLa cells were prepared as described in et al.58.	HeLa suggested: None BHK-21 suggested: ATCC Cat# CRL-6281, RRID:CVCL_1914)
   Influenza-infected MDCK cells (see Supplementary Fig. 9) were prepared as described by Kim et al.59 and the images were captured with a Synentec CellaVista35 at 20x.	MDCK suggested: None
   Software and Algorithms
   Sentences	Resources
   Bootstrapping on the knowledge acquired from those models allowed the AVIA models to converge faster and at a higher accuracy 64.	AVIA suggested: (AVIA, RRID:SCR_005172)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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3. 

   Version published to 10.1101/2022.03.23.485512 on bioRxiv

   Mar 28, 2022