Microbiology

Inner membrane complex proteomics reveals a palmitoylation regulation critical for intraerythrocytic development of malaria parasite

1. Pengge Qian
2. Xu Wang
3. Chuan-Qi Zhong
4. Jiaxu Wang
5. Mengya Cai
6. Wang Nguitragool
7. Jian Li
8. Huiting Cui
9. Jing Yuan

• Curated by eLife

  Evaluation Summary:

  This paper is of interest to scientists within the field of apicomplexans cytoskeleton and malaria parasite proliferation. A series of compelling experimental manipulations identify potential new pellicle proteins and dissect the role of a protein acyl-transferase for the development of the intraerythrocytic stages of Plasmodiun yoelii and the palmitoylation status of two potential substrates.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Understanding drivers of phylogenetic clustering and terminal branch lengths distribution in epidemics of Mycobacterium tuberculosis

1. Fabrizio Menardo

• Curated by eLife

  Evaluation Summary:

  This is an interesting simulation-based study focusing on the genomic epidemiology of Mycobacterium tuberculosis. The work nicely relates key biological and epidemiological parameters to how M. tuberculosis isolates cluster together, and to the terminal branch lengths in M. tuberculosis phylogenies. These concepts have both been applied to comparative studies of M. tuberculosis success and have often been interpreted as reflecting differences in transmission. The author finds that clustering and terminal branch lengths can also be modified by differences in the latent period, the mutation rate or the sampling fraction. This work will be of broad interest to readers studying tuberculosis epidemiology and transmission modelling.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Differential Pathogenesis of SARS-CoV-2 Variants of Concern in Human ACE2-Expressing Mice

1. Janhavi Prasad Natekar
2. Heather Pathak
3. Shannon Stone
4. Pratima Kumari
5. Shaligram Sharma
6. Tabassum Tasnim Auroni
7. Komal Arora
8. Hussin Alwan Rothan
9. Mukesh Kumar

Reviewed by ScreenIT

The SARS-CoV-2 spike S375F mutation characterizes the Omicron BA.1 variant

1. Izumi Kimura
2. Daichi Yamasoba
3. Hesham Nasser
4. Jiri Zahradnik
5. Yusuke Kosugi
6. Jiaqi Wu
7. Kayoko Nagata
8. Keiya Uriu
9. Yuri L. Tanaka
10. Jumpei Ito
11. Ryo Shimizu
12. Toong Seng Tan
13. Erika P. Butlertanaka
14. Hiroyuki Asakura
15. Kenji Sadamasu
16. Kazuhisa Yoshimura
17. Takamasa Ueno
18. Akifumi Takaori-Kondo
19. Gideon Schreiber
20. Mako Toyoda
21. Kotaro Shirakawa
22. Takashi Irie
23. Akatsuki Saito
24. So Nakagawa
25. Terumasa Ikeda
26. Kei Sato

Reviewed by ScreenIT

Evolution of the Quorum Sensing Regulon in Cooperating Populations of Pseudomonas aeruginosa

1. Nicole E. Smalley
2. Amy L. Schaefer
3. Kyle L. Asfahl
4. Crystal Perez
5. E. Peter Greenberg
6. Ajai A. Dandekar

• Curated by eLife

  Evaluation Summary:

  Pseudomonas aeruginosa regulates the production of many cooperative traits through quorum sensing cell-cell signaling. The authors carried out transcriptomic studies of experimental evolved populations of P. aeruginosa and observed that the size of the quorum sensing regulon decreases when only a few but not all the cooperative processes regulated quorum sensing are required for growth. Their findings are consistent with the hypothesis that quorum sensing regulated genes can be counter selected rapidly when not beneficial. This study is of interest for microbiologists studying quorum-sensing, and evolutionary biologists studying the evolution of cooperative behavior.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

SARS-CoV-2 Omicron is specifically restricted in its replication in human lung tissue, compared to other variants of concern

1. Or Alfi
2. Marah Hamdan
3. Ori Wald
4. Arkadi Yakirevitch
5. Ori Wandel
6. Esther Oiknine-Djian
7. Ben Gvili
8. Hadas Knoller
9. Noa Rozendorn
10. Hadar Golan
11. Sheera Adar
12. Olesya Vorontsov
13. Michal Mandelboim
14. Zichria Zakay-Rones
15. Menachem Oberbaum
16. Amos Panet
17. Dana G. Wolf

Reviewed by ScreenIT

Brequinar and dipyridamole in combination exhibits synergistic antiviral activity against SARS-CoV-2 in vitro: Rationale for a host-acting antiviral treatment strategy for COVID-19

1. James F. Demarest
2. Maryline Kienle
3. RuthMabel Boytz
4. Mary Ayres
5. Eun Jung Kim
6. J.J. Patten
7. Donghoon Chung
8. Varsha Gandhi
9. Robert A. Davey
10. David B. Sykes
11. Nadim Shohdy
12. John C. Pottage
13. Vikram S. Kumar

Reviewed by ScreenIT

Cross-neutralization of Omicron BA.1 against BA.2 and BA.3 SARS-CoV-2

1. Jing Zou
2. Chaitanya Kurhade
3. Hongjie Xia
4. Mingru Liu
5. Xuping Xie
6. Ping Ren
7. Pei-Yong Shi

Reviewed by ScreenIT

Methylene blue, Mycophenolic acid, Posaconazole, and Niclosamide inhibit SARS-CoV-2Omicron variant BA.1 infection of human airway epithelial explant cultures

1. Romain Volle
2. Luca Murer
3. Anthony Petkidis
4. Vardan Andriasyan
5. Alessandro Savi
6. Cornelia Bircher
7. Nicole Meili
8. Lucy Fischer
9. Daniela Policarpo Sequeira
10. Daniela Katharina Mark
11. Alfonso Gomez-Gonzalez
12. Urs F. Greber

Reviewed by ScreenIT

Neutralization of Omicron BA.1, BA.2, and BA.3 SARS-CoV-2 by 3 doses of BNT162b2 vaccine

1. Chaitanya Kurhade
2. Jing Zou
3. Hongjie Xia
4. Hui Cai
5. Qi Yang
6. Mark Cutler
7. David Cooper
8. Alexander Muik
9. Kathrin U. Jansen
10. Xuping Xie
11. Kena A. Swanson
12. Pei‑Yong Shi

Reviewed by ScreenIT