Microbiology

Antibody Resistance of SARS-CoV-2 Omicron BA.1, BA.1.1, BA.2 and BA.3 Sub-lineages

1. Jingwen Ai
2. Xun Wang
3. Xinyi He
4. Xiaoyu Zhao
5. Yi Zhang
6. Yuchao Jiang
7. Minghui Li
8. Yuchen Cui
9. Yanjia Chen
10. Rui Qiao
11. Lin Li
12. Lulu Yang
13. Yi Li
14. Zixin Hu
15. Wenhong Zhang
16. Pengfei Wang

Reviewed by ScreenIT

Delta-Omicron recombinant escapes therapeutic antibody neutralization

1. Ralf Duerr
2. Hao Zhou
3. Takuya Tada
4. Dacia Dimartino
5. Christian Marier
6. Paul Zappile
7. Guiqing Wang
8. Jonathan Plitnick
9. Sara B. Griesemer
10. Roxanne Girardin
11. Jessica Machowski
12. Sean Bialosuknia
13. Erica Lasek-Nesselquist
14. Samuel L. Hong
15. Guy Baele
16. Meike Dittmann
17. Mila B. Ortigoza
18. Prithiv J. Prasad
19. Kathleen McDonough
20. Nathaniel R. Landau
21. Kirsten St George
22. Adriana Heguy

Reviewed by ScreenIT

Thermosynechococcus switches the direction of phototaxis by a c-di-GMP-dependent process with high spatial resolution

1. Daisuke Nakane
2. Gen Enomoto
3. Heike Bähre
4. Yuu Hirose
5. Annegret Wilde
6. Takayuki Nishizaka

• Curated by eLife

  Evaluation Summary:

  This manuscript by Nakane et al investigates phototaxis of the rod shaped bacteria Thermosynechococcus vulcanus. This is important because most our knowledge on phototaxis is only emerging on round-shaped cyanobacteria. In the study, the authors demonstrate that T. vulcanus can chemotax positively or negatively to light depending on the light source. They identify a photoreceptor complex that drives negative phototaxis and propose that it impacts motility by increasing cdiGMP levels, which in turn would regulate the motility complex, formed by bi-polar Type-IV pili. Provided that the link between light induced cdi-GMP and spatial TFP activity is established, the work would provide a new mechanistic framework to explain TFP-driven phototaxis. This is an important topic because TFPs are emerging as spatially-regulated motility machineries in a large number of bacterial systems and linking their activity to receptors and secondary messengers is a current unresolved question.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Targeted protein S-nitrosylation of ACE2 inhibits SARS-CoV-2 infection

1. Chang-ki Oh
2. Tomohiro Nakamura
3. Nathan Beutler
4. Xu Zhang
5. Juan Piña-Crespo
6. Maria Talantova
7. Swagata Ghatak
8. Dorit Trudler
9. Lauren N. Carnevale
10. Scott R. McKercher
11. Malina A. Bakowski
12. Jolene K. Diedrich
13. Amanda J. Roberts
14. Ashley K. Woods
15. Victor Chi
16. Anil K. Gupta
17. Mia A. Rosenfeld
18. Fiona L. Kearns
19. Lorenzo Casalino
20. Namir Shaabani
21. Hejun Liu
22. Ian A. Wilson
23. Rommie E. Amaro
24. Dennis R. Burton
25. John R. Yates
26. Cyrus Becker
27. Thomas F. Rogers
28. Arnab K. Chatterjee
29. Stuart A. Lipton

Reviewed by ScreenIT

Screening of SARS-CoV-2 antivirals through a cell-based RNA-dependent RNA polymerase (RdRp) reporter assay

1. Timsy Uppal
2. Kai Tuffo
3. Svetlana Khaiboullina
4. Sivani Reganti
5. Mark Pandori
6. Subhash C. Verma

Reviewed by ScreenIT

Inner membrane complex proteomics reveals a palmitoylation regulation critical for intraerythrocytic development of malaria parasite

1. Pengge Qian
2. Xu Wang
3. Chuan-Qi Zhong
4. Jiaxu Wang
5. Mengya Cai
6. Wang Nguitragool
7. Jian Li
8. Huiting Cui
9. Jing Yuan

• Curated by eLife

  Evaluation Summary:

  This paper is of interest to scientists within the field of apicomplexans cytoskeleton and malaria parasite proliferation. A series of compelling experimental manipulations identify potential new pellicle proteins and dissect the role of a protein acyl-transferase for the development of the intraerythrocytic stages of Plasmodiun yoelii and the palmitoylation status of two potential substrates.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Understanding drivers of phylogenetic clustering and terminal branch lengths distribution in epidemics of Mycobacterium tuberculosis

1. Fabrizio Menardo

• Curated by eLife

  Evaluation Summary:

  This is an interesting simulation-based study focusing on the genomic epidemiology of Mycobacterium tuberculosis. The work nicely relates key biological and epidemiological parameters to how M. tuberculosis isolates cluster together, and to the terminal branch lengths in M. tuberculosis phylogenies. These concepts have both been applied to comparative studies of M. tuberculosis success and have often been interpreted as reflecting differences in transmission. The author finds that clustering and terminal branch lengths can also be modified by differences in the latent period, the mutation rate or the sampling fraction. This work will be of broad interest to readers studying tuberculosis epidemiology and transmission modelling.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Differential Pathogenesis of SARS-CoV-2 Variants of Concern in Human ACE2-Expressing Mice

1. Janhavi Prasad Natekar
2. Heather Pathak
3. Shannon Stone
4. Pratima Kumari
5. Shaligram Sharma
6. Tabassum Tasnim Auroni
7. Komal Arora
8. Hussin Alwan Rothan
9. Mukesh Kumar

Reviewed by ScreenIT

The SARS-CoV-2 spike S375F mutation characterizes the Omicron BA.1 variant

1. Izumi Kimura
2. Daichi Yamasoba
3. Hesham Nasser
4. Jiri Zahradnik
5. Yusuke Kosugi
6. Jiaqi Wu
7. Kayoko Nagata
8. Keiya Uriu
9. Yuri L. Tanaka
10. Jumpei Ito
11. Ryo Shimizu
12. Toong Seng Tan
13. Erika P. Butlertanaka
14. Hiroyuki Asakura
15. Kenji Sadamasu
16. Kazuhisa Yoshimura
17. Takamasa Ueno
18. Akifumi Takaori-Kondo
19. Gideon Schreiber
20. Mako Toyoda
21. Kotaro Shirakawa
22. Takashi Irie
23. Akatsuki Saito
24. So Nakagawa
25. Terumasa Ikeda
26. Kei Sato

Reviewed by ScreenIT

Evolution of the Quorum Sensing Regulon in Cooperating Populations of Pseudomonas aeruginosa

1. Nicole E. Smalley
2. Amy L. Schaefer
3. Kyle L. Asfahl
4. Crystal Perez
5. E. Peter Greenberg
6. Ajai A. Dandekar

• Curated by eLife

  Evaluation Summary:

  Pseudomonas aeruginosa regulates the production of many cooperative traits through quorum sensing cell-cell signaling. The authors carried out transcriptomic studies of experimental evolved populations of P. aeruginosa and observed that the size of the quorum sensing regulon decreases when only a few but not all the cooperative processes regulated quorum sensing are required for growth. Their findings are consistent with the hypothesis that quorum sensing regulated genes can be counter selected rapidly when not beneficial. This study is of interest for microbiologists studying quorum-sensing, and evolutionary biologists studying the evolution of cooperative behavior.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife