Microbiology

Soluble MAC is primarily released from MAC-resistant bacteria that potently convert complement component C5

1. Dennis J Doorduijn
2. Marie V Lukassen
3. Marije FL van 't Wout
4. Vojtech Franc
5. Maartje Ruyken
6. Bart W Bardoel
7. Albert JR Heck
8. Suzan HM Rooijakkers

• Curated by eLife

  Evaluation Summary:

  The soluble membrane attack complex (sMAC) is generated from complement activation and contains the complement proteins C5b, C6, C7, C8, C9 together with the regulatory proteins clusterin and vitronectin. Despite intense interest in sMAC, the mechanisms regulating its formation remain poorly understood. In this manuscript the authors demonstrate that sMAC is formed when complement is activated on bacteria that are resistant to killing by MAC pores.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Gas6 drives Zika virus-induced neurological complications in humans and congenital syndrome in immunocompetent mice

1. Joao Luiz Silva-Filho
2. Lilian G. de Oliveira
3. Leticia Monteiro
4. Pierina L. Parise
5. Nagela G. Zanluqui
6. Carolina M. Polonio
7. Carla L. de Freitas
8. Daniel A. Toledo-Teixeira
9. William M. de Souza
10. Najara Bittencourt
11. Mariene R. Amorim
12. Julia Forato
13. Stéfanie P. Muraro
14. Gabriela F. de Souza
15. Matheus C. Martini
16. Karina Bispo-dos-Santos
17. Aline Vieira
18. Carla C. Judice
19. Glaucia M. Pastore
20. Eliana Amaral
21. Renato Passini Junior
22. Helaine M.B.P. Mayer-Milanez
23. Carolina C. Ribeiro-do-Valle
24. Roseli Calil
25. João Renato Bennini Junior
26. Giuliane J. Lajos
27. Albina Altemani
28. Marcos T. Nolasco da Silva
29. Ana Carolina Coan
30. Maria Francisca Colella-Santos
31. Andrea P.B. von Zuben
32. Marco Aurélio R. Vinolo
33. Clarice Weis Arns
34. Rodrigo Ramos Catharino
35. Maria Laura Costa
36. Rodrigo N. Angerami
37. André R.R. Freitas
38. Mariangela R. Resende
39. Márcia T. Garcia
40. Maria Luiza Moretti
41. Laurent Renia
42. Lisa F.P. Ng
43. Carla V. Rothlin
44. Fabio T.M. Costa
45. Jean Pierre Schatzmann Peron
46. José Luiz Proença-Modena

• Curated by eLife

  Evaluation Summary:

  The pathophysiology of Zika virus infection remains an area of high research interest. In this study, the authors use diverse experimental models to examine the potential role of Gas6 in Zika virus infection and associated neurological sequelae.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Nsp1 proteins of human coronaviruses HCoV-OC43 and SARS-CoV2 inhibit stress granule formation

1. Stacia M. Dolliver
2. Mariel Kleer
3. Maxwell P. Bui-Marinos
4. Shan Ying
5. Jennifer A. Corcoran
6. Denys A. Khaperskyy

Reviewed by ScreenIT

The highly conserved stem-loop II motif is important for the lifecycle of astroviruses but dispensable for SARS-CoV-2

1. Andrew B Janowski
2. Hongbing Jiang
3. Chika Fujii
4. Macee C Owen
5. Traci L Bricker
6. Tamarand L Darling
7. Houda H. Harastani
8. Kuljeet Seehra
9. Stephen Tahan
10. Ana Jung
11. Binita Febles
12. Joshua A Blatter
13. Scott A Handley
14. Bijal A Parikh
15. Valeria Lulla
16. Adrianus CM Boon
17. David Wang

Reviewed by ScreenIT

A Bacteriophage-Based, Highly Efficacious, Needle- and Adjuvant-Free, Mucosal COVID-19 Vaccine

1. Jingen Zhu
2. Swati Jain
3. Jian Sha
4. Himanshu Batra
5. Neeti Ananthaswamy
6. Paul B. Kilgore
7. Emily K. Hendrix
8. Yashoda M. Hosakote
9. Xiaorong Wu
10. Juan P. Olano
11. Adeyemi Kayode
12. Cristi L. Galindo
13. Simran Banga
14. Aleksandra Drelich
15. Vivian Tat
16. Chien-Te K. Tseng
17. Ashok K. Chopra
18. Venigalla B. Rao

Reviewed by ScreenIT

Removal of clinically relevant SARS-CoV-2 variants by an affinity resin containing Galanthus nivalis agglutinin

1. Melanie Gooldy
2. Christelle M. Roux
3. Steven P. LaRosa
4. Nicole Spaulding
5. Charles J. Fisher

Reviewed by ScreenIT

Structural and functional characteristics of SARS-CoV-2 Omicron subvariant BA.2 spike

1. Jun Zhang
2. Weichun Tang
3. Hailong Gao
4. Christy L. Lavine
5. Wei Shi
6. Hanqin Peng
7. Haisun Zhu
8. Krishna Anand
9. Matina Kosikova
10. Hyung Joon Kwon
11. Pei Tong
12. Avneesh Gautam
13. Sophia Rits-Volloch
14. Shaowei Wang
15. Megan L. Mayer
16. Duane R. Wesemann
17. Michael S. Seaman
18. Jianming Lu
19. Tianshu Xiao
20. Hang Xie
21. Bing Chen

Reviewed by ScreenIT

The spike gene is a major determinant for the SARS-CoV-2 Omicron-BA.1 phenotype

1. G. Tuba Barut
2. Nico Joel Halwe
3. Adriano Taddeo
4. Jenna N. Kelly
5. Jacob Schön
6. Nadine Ebert
7. Lorenz Ulrich
8. Christelle Devisme
9. Silvio Steiner
10. Bettina Salome Trüeb
11. Bernd Hoffmann
12. Inês Berenguer Veiga
13. Nathan Georges François Leborgne
14. Etori Aguiar Moreira
15. Angele Breithaupt
16. Claudia Wylezich
17. Dirk Höper
18. Kerstin Wernike
19. Aurélie Godel
20. Lisa Thomann
21. Vera Flück
22. Hanspeter Stalder
23. Melanie Brügger
24. Blandina I. Oliveira Esteves
25. Beatrice Zumkehr
26. Guillaume Beilleau
27. Annika Kratzel
28. Kimberly Schmied
29. Sarah Ochsenbein
30. Reto M. Lang
31. Manon Wider
32. Carlos Machahua
33. Patrick Dorn
34. Thomas M. Marti
35. Manuela Funke-Chambour
36. Andri Rauch
37. Marek Widera
38. Sandra Ciesek
39. Ronald Dijkman
40. Donata Hoffmann
41. Marco P. Alves
42. Charaf Benarafa
43. Martin Beer
44. Volker Thiel

Reviewed by ScreenIT

P681 mutations within the polybasic motif of spike dictate fusogenicity and syncytia formation of SARS CoV-2 variants

1. Alona Kuzmina
2. Nofar Atari
3. Aner Ottolenghi
4. Dina Korovin
5. Ido Cohen lass
6. Benyamin Rosental
7. Elli Rosenberg
8. Michal Mandelboim
9. Ran Taube

Reviewed by ScreenIT

Targeted genomic sequencing with probe capture for discovery and surveillance of coronaviruses in bats

1. Kevin S Kuchinski
2. Kara D Loos
3. Danae M Suchan
4. Jennifer N Russell
5. Ashton N Sies
6. Charles Kumakamba
7. Francisca Muyembe
8. Placide Mbala Kingebeni
9. Ipos Ngay Lukusa
10. Frida N’Kawa
11. Joseph Atibu Losoma
12. Maria Makuwa
13. Amethyst Gillis
14. Matthew LeBreton
15. James A Ayukekbong
16. Nicole A Lerminiaux
17. Corina Monagin
18. Damien O Joly
19. Karen Saylors
20. Nathan D Wolfe
21. Edward M Rubin
22. Jean J Muyembe Tamfum
23. Natalie A Prystajecky
24. David J McIver
25. Christian E Lange
26. Andrew DS Cameron

• Curated by eLife

  Evaluation Summary:

  This work applies hybrid-capture sequencing for coronavirus (CoV) surveillance in bats. Given that bats are a major reservoir for animal-to-human virus spillover events, which have caused several major epidemics/pandemics, this is a very important field of research. The reported hybrid-capture method shows some clear advantages over amplicon-based viral sequencing, which is the established standard in the field. This new approach has clear merits that are well supported by the data presented and is likely to become an important tool in viral surveillance programs that ultimately aim to predict/prevent/prepare for future pandemics. The work will be of interest to microbiologists, particularly those studying viruses or interested in genomics surveillance.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife, ScreenIT