Microbiology

SARS-ANI: a global open access dataset of reported SARS-CoV-2 events in animals

1. Afra Nerpel
2. Liuhuaying Yang
3. Johannes Sorger
4. Annemarie Käsbohrer
5. Chris Walzer
6. Amélie Desvars-Larrive

Reviewed by ScreenIT

The interferon-inducible GTPase MxB promotes capsid disassembly and genome release of herpesviruses

1. Manutea C Serrero
2. Virginie Girault
3. Sebastian Weigang
4. Todd M Greco
5. Ana Ramos-Nascimento
6. Fenja Anderson
7. Antonio Piras
8. Ana Hickford Martinez
9. Jonny Hertzog
10. Anne Binz
11. Anja Pohlmann
12. Ute Prank
13. Jan Rehwinkel
14. Rudolf Bauerfeind
15. Ileana M Cristea
16. Andreas Pichlmair
17. Georg Kochs
18. Beate Sodeik

• Curated by eLife

  Evaluation Summary:

  This paper uses an innovative cell-free system to identify antiviral factors that interact with HSV-1 in cells. In addition to cataloging many capsid-interacting factors, the paper probes the antiviral mechanism of one of these, MxB. The data provide strong support for an intriguing model in which MxB "punches" holes in HSV-1 capsids, releasing viral DNA and potentially triggering host DNA sensors. However, the binding of a variety of factors to the capsid appears able to bind to and shield the capsids from MxB attack, suggesting a new perspective on how viruses might evade some host defenses.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Phosphoregulation accommodates Type III secretion and assembly of a tether of ER-Chlamydia inclusion membrane contact sites

1. Rachel J Ende
2. Rebecca L Murray
3. Samantha K D'Spain
4. Isabelle Coppens
5. Isabelle Derré

• Curated by eLife

  Evaluation Summary:

  This manuscript will be of interest to readers in the field of infection and cell biology. The authors follow up their previous study to further deepen our understanding of host-pathogen interactions that contribute to transkingdom contact sites. The authors show that the Chlamydia effector protein IncV tethers ER to the bacterial vacuole in a manner dependent on the phosphorylation of its C-terminus by the host kinase CK2. In addition, the authors show that IncV recruits the host kinase CK2 to the bacterial vacuole in manner required for its phosphorylation and ER tethering. Overall, the data justify most of the key claims put forward by the authors. Nonetheless, clarification is required concerning the chain of events and the phosphorylation events required for VAP binding.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

The Delta variant SARS-CoV-2 spike protein uniquely promotes aggregation of pseudotyped viral particles

1. Jennifer D. Petersen
2. Jianming Lu
3. Wendy Fitzgerald
4. Fei Zhou
5. Paul S. Blank
6. Doreen Matthies
7. Joshua Zimmerberg

Reviewed by ScreenIT

Hippo signaling pathway activation during SARS-CoV-2 infection contributes to host antiviral response

1. Gustavo Garcia
2. Arjit Vijey Jeyachandran
3. Yijie Wang
4. Joseph Ignatius Irudayam
5. Sebastian Castillo Cario
6. Chandani Sen
7. Shen Li
8. Yunfeng Li
9. Ashok Kumar
10. Karin Nielsen-Saines
11. Samuel W. French
12. Priya S. Shah
13. Kouki Morizono
14. Brigitte N. Gomperts
15. Arjun Deb
16. Arunachalam Ramaiah
17. Vaithilingaraja Arumugaswami

Reviewed by ScreenIT

Antibody Resistance of SARS-CoV-2 Omicron BA.1, BA.1.1, BA.2 and BA.3 Sub-lineages

1. Jingwen Ai
2. Xun Wang
3. Xinyi He
4. Xiaoyu Zhao
5. Yi Zhang
6. Yuchao Jiang
7. Minghui Li
8. Yuchen Cui
9. Yanjia Chen
10. Rui Qiao
11. Lin Li
12. Lulu Yang
13. Yi Li
14. Zixin Hu
15. Wenhong Zhang
16. Pengfei Wang

Reviewed by ScreenIT

Delta-Omicron recombinant escapes therapeutic antibody neutralization

1. Ralf Duerr
2. Hao Zhou
3. Takuya Tada
4. Dacia Dimartino
5. Christian Marier
6. Paul Zappile
7. Guiqing Wang
8. Jonathan Plitnick
9. Sara B. Griesemer
10. Roxanne Girardin
11. Jessica Machowski
12. Sean Bialosuknia
13. Erica Lasek-Nesselquist
14. Samuel L. Hong
15. Guy Baele
16. Meike Dittmann
17. Mila B. Ortigoza
18. Prithiv J. Prasad
19. Kathleen McDonough
20. Nathaniel R. Landau
21. Kirsten St George
22. Adriana Heguy

Reviewed by ScreenIT

Thermosynechococcus switches the direction of phototaxis by a c-di-GMP-dependent process with high spatial resolution

1. Daisuke Nakane
2. Gen Enomoto
3. Heike Bähre
4. Yuu Hirose
5. Annegret Wilde
6. Takayuki Nishizaka

• Curated by eLife

  Evaluation Summary:

  This manuscript by Nakane et al investigates phototaxis of the rod shaped bacteria Thermosynechococcus vulcanus. This is important because most our knowledge on phototaxis is only emerging on round-shaped cyanobacteria. In the study, the authors demonstrate that T. vulcanus can chemotax positively or negatively to light depending on the light source. They identify a photoreceptor complex that drives negative phototaxis and propose that it impacts motility by increasing cdiGMP levels, which in turn would regulate the motility complex, formed by bi-polar Type-IV pili. Provided that the link between light induced cdi-GMP and spatial TFP activity is established, the work would provide a new mechanistic framework to explain TFP-driven phototaxis. This is an important topic because TFPs are emerging as spatially-regulated motility machineries in a large number of bacterial systems and linking their activity to receptors and secondary messengers is a current unresolved question.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Targeted protein S-nitrosylation of ACE2 inhibits SARS-CoV-2 infection

1. Chang-ki Oh
2. Tomohiro Nakamura
3. Nathan Beutler
4. Xu Zhang
5. Juan Piña-Crespo
6. Maria Talantova
7. Swagata Ghatak
8. Dorit Trudler
9. Lauren N. Carnevale
10. Scott R. McKercher
11. Malina A. Bakowski
12. Jolene K. Diedrich
13. Amanda J. Roberts
14. Ashley K. Woods
15. Victor Chi
16. Anil K. Gupta
17. Mia A. Rosenfeld
18. Fiona L. Kearns
19. Lorenzo Casalino
20. Namir Shaabani
21. Hejun Liu
22. Ian A. Wilson
23. Rommie E. Amaro
24. Dennis R. Burton
25. John R. Yates
26. Cyrus Becker
27. Thomas F. Rogers
28. Arnab K. Chatterjee
29. Stuart A. Lipton

Reviewed by ScreenIT

Screening of SARS-CoV-2 antivirals through a cell-based RNA-dependent RNA polymerase (RdRp) reporter assay

1. Timsy Uppal
2. Kai Tuffo
3. Svetlana Khaiboullina
4. Sivani Reganti
5. Mark Pandori
6. Subhash C. Verma

Reviewed by ScreenIT