Genetics

Sickle cell status skews malaria parasite genotype at infection

1. Helena D. Hopson
2. Alejandra Herbert-Mainero
3. Aline Gaelle Bouopda-Tuedom
4. Charlène Tina Nanssong-Vomo
5. Brigitte Tumamo Fotso
6. Belinda Claire Kiam
7. Ibrahima Ibrahima
8. Clément Onguene
9. Luc Abate
10. Radoslaw Igor Omelianczyk
11. Heather D. Evans
12. Lyra E. Horton
13. Gavin Band
14. Tracey J. Lamb
15. Lawrence S. Ayong
16. Sandrine E. Nsango
17. Ellen M. Leffler

Reviewed by Rapid Reviews Infectious Diseases

Visible traits demonstrate that crispant founder mice can be used for phenotypic assessment

1. Rebekah Tillotson
2. Marina Gertsenstein
3. Li-Hsin Chang
4. Julie Ruston
5. Fernando Bellido Molías
6. Lauri G Lintott
7. Christine Taylor
8. Philippe Gautier
9. Lauryl MJ Nutter
10. Monica J Justice

• Curated by eLife

  eLife Assessment

  This study offers important methodological advances for CRISPR-based mutagenesis in mice, highlighting the potential of founder animals for early phenotypic characterization. The authors present convincing evidence, supported by rigorous experimental design, that "crispant" (F0) analysis in mice, despite prior concerns about genetic mosaicism, can be utilized to assess protein function.

Reviewed by eLife

Functional Profiling of 2,193 ASS1 Missense Variants: Insights into Variant Pathogenicity and Epistatic Interactions in Citrullinemia Type I

1. Russell S. Lo
2. Gareth A. Cromie
3. Michelle Tang
4. Amy Sirr
5. Ljubica Caldovic
6. Hiroki Morizono
7. Nicholas Ah Mew
8. Andrea Gropman
9. Aimée M. Dudley

Reviewed by Review Commons

Reproducibility of Scientific Claims in Drosophila Immunity: A Retrospective Analysis of 400 Publications

1. Hannah Westlake
2. Fabrice David
3. Yao Tian
4. Kenan Krakovic
5. Asya Dolgikh
6. Liza Juravlev
7. Thomas Esmangart de Bournonville
8. Alexia Carboni
9. Claudia Melcarne
10. Tisheng Shan
11. Yang Wang
12. Yizhu Mu
13. Akshata Kotwal
14. Nadia Pirko
15. Jean Philippe Boquete
16. Fanny Schüpfer
17. Samuel Rommelaere
18. Mickael Poidevin
19. Zhonggeng Liu
20. Shu Kondo
21. Girish S Ratnaparkhi
22. Sveta Chakrabarti
23. Guiqing Liu
24. Florent Masson
25. Li Xiaoxue
26. Mark A Hanson
27. Haobo Jiang
28. Francesca Di Cara
29. Estee Kurant
30. Bruno Lemaitre

• Curated by eLife

  eLife Assessment

  This fundamental study is part of an impressive, large-scale effort to assess the reproducibility of published findings in the field of Drosophila immunity. In a companion article, the authors analyze 400 papers published between 1959 and 2011, and assess how many of the claims in these papers have been tested in subsequent publications. In this article, the authors report the results of validation experiments to assess a subset of the claims that, according to the literature, have not been corroborated. While the evidence reported for some of these validation studies is convincing, it remains incomplete for others.

Reviewed by eLife

Genetic and environmental determinants of multicellular-like phenotypes in fission yeast

1. Bence Kӧvér
2. Céleste E Cohen
3. Ladislav Seres
4. Saniya Raut
5. Markus Ralser
6. Benjamin M Heineike
7. Jürg Bähler

Reviewed by Review Commons

Khdc3 Regulates Metabolism Across Generations in a DNA-Independent Manner

1. Liana Senaldi
2. Nora Hassan
3. Sean Cullen
4. Uthra Balaji
5. Natalie Trigg
6. Jinghua Gu
7. Hailey Finkelstein
8. Kathryn Phillips
9. Colin Conine
10. Matthew Smith-Raska

• Curated by eLife

  eLife Assessment

  This important mouse study shows that wild-type female progeny of Khdc3 mutants have abnormal gene expression relating to hepatic metabolism, which persists over multiple generations and passes through both female and male lineages. A role for small RNAs on this phenomenon is proposed, and evidence supporting the authors' claims is convincing. Further experiments are required to functionally validate the role of small RNAs in transmission of the phenotype. The work will be of interest to researchers in the field of DNA-independent mechanism of inheritance.

Reviewed by eLife

A default silencing mechanism restrains stress-induced genes in C. elegans

1. Orkan Ilbay
2. Alejandro Rodriguez Gama
3. Daniel F Jarosz
4. Richard I Morimoto
5. Andrew Fire

• Curated by eLife

  eLife Assessment

  This valuable study identifies a novel regulator of stress-induced gene quiescence in C. elegans: the multi-Zinc-finger protein ZNF-236. The work provides evidence for an active mechanism that maintains the repressed state of inducible genes under basal conditions in the absence of stress. The claims for discovery made in the title and abstract are supported by solid experimental data. However, a deeper investigation into the mechanisms of ZNF-236 action could substantially enhance the manuscript's impact and value.

Reviewed by eLife