Cell Biology

Editing of endogenous tubulins reveals varying effects of tubulin posttranslational modifications on axonal growth and regeneration

1. Yu-Ming Lu
2. Shan Yan
3. Shih-Chieh Ti
4. Chaogu Zheng

• Curated by eLife

  eLife assessment

  This fundamental study analyzes the roles of post-translational modifications of tubulin by generating a large panel of tubulin mutants and describing their effects on morphogenesis and function of sensory neurons in C. elegans. The work, which is of interest to all cell biologists, in particular researchers with an interest in the microtubule cytoskeleton and neurobiology, presents conclusions that are supported by solid evidence. Demonstrating that all introduced mutations have the intended consequences and exploring their direct effect on microtubules would further increase the impact of the work.

Reviewed by eLife

Heparan sulfate-dependent phase separation of CCL5 and its chemotactic activity

1. Xiaolin Yu
2. Guangfei Duan
3. Pengfei Pei
4. Long Chen
5. Renji Gu
6. Wenrui Hu
7. Hongli Zhang
8. Yan-Dong Wang
9. Lili Gong
10. Lihong Liu
11. Ting-Ting Chu
12. Jin-Ping Li
13. Shi-Zhong Luo

• Curated by eLife

  eLife assessment

  How the triplicate interaction between chemokines with both GAGs and G protein-coupled receptors (GPCR) works and how gradients are created and potentially maintained in vivo are poorly understood. The authors provide solid evidence to show phase separation can drive chemotactic gradient formation. The paper is a useful advance in the field of chemokine biology.

Reviewed by eLife

The endoplasmic reticulum stress sensor IRE1 regulates collagen secretion through the enforcement of the proteostasis factor P4HB/PDIA1 contributing to liver damage and fibrosis

1. Younis Hazari
2. Hery Urra
3. Valeria A. Garcia Lopez
4. Javier Diaz
5. Giovanni Tamburini
6. Mateus Milani
7. Philippe Pihan
8. Sylvere Durand
9. Fanny Aprahamia
10. Reese Baxter
11. Menghao Huang
12. X Charlie Dong
13. Helena Vihinen
14. Ana Batista-Gonzalez
15. Patricio Godoy
16. Alfredo Criollo
17. Vlad Ratziu
18. Fabienne Foufelle
19. Jan G. Hengstler
20. Eija Jokitalo
21. Beatrice Bailly-maitre
22. Jessica L Maiers
23. Lars Plate
24. Guido Kroemer
25. Claudio Hetz

Reviewed by Review Commons

Novel Nuclear Roles for Testis-Specific ACTL7A and ACTL7B Supported by In Vivo Characterizations and AI Facilitated In Silico Mechanistic Modeling with Implications for Epigenetic Regulation in Spermiogenesis

1. Pierre Ferrer
2. Srijana Upadhyay
3. James J. Cai
4. Tracy M. Clement

• Curated by eLife

  eLife assessment

  This valuable study reports that actin-related proteins may be involved in transcriptional regulation during spermatogenesis. The supporting data remain incomplete, and more extensive disentanglement from the canonical role of these actin-related proteins and the experimental validation of in silico predictions are required. This work will be of interest to reproductive biologists and other researchers working on non-canonical roles of actin and actin-related proteins.

Reviewed by eLife

Temporally controlled nervous system-to-gut signaling bidirectionally regulates longevity in C. elegans

1. Lingxiu Xu
2. Chengxuan Han
3. Lei Chun
4. X.Z. Shawn Xu
5. Jianfeng Liu

• Curated by eLife

  eLife assessment

  This study reports that the timing of 'brain-to-gut' signaling influences the lifespan of the C. elegans model. The main finding, that modulating the same neurotransmitter, Acetylcholine, at different ages elicits lifespan shortening - or extending - effects utilizing different receptors, is important and of broad interest to the longevity field as recognized by all the reviewers. The data is largely consistent with the authors' model, but the strength of the evidence is incomplete. The study requires several rigorous experiments detailed by the reviewers to substantiate the main conclusions.

Reviewed by eLife

O-GlcNAcylation of the intellectual disability protein DDX3X exerts proteostatic cell cycle control

1. Conor W. Mitchell
2. Huijie Yuan
3. Marie Sønderstrup-Jensen
4. Florence Authier
5. Alfonso Manuel D’Alessio
6. Andrew T. Ferenbach
7. Daan M.F. van Aalten

Reviewed by Review Commons

Mir221/222 drive synovial hyperplasia and arthritis by targeting cell cycle inhibitors and chromatin remodeling components

1. Fani Roumelioti
2. Christos Tzaferis
3. Dimitris Konstantopoulos
4. Dimitra Papadopoulou
5. Alejandro Prados
6. Maria Sakkou
7. Anastasios Liakos
8. Panagiotis Chouvardas
9. Theodore Meletakos
10. Yiannis Pandis
11. Niki Karagianni
12. Maria C Denis
13. Maria Fousteri
14. Maria Armaka
15. George Kollias

• Curated by eLife

  eLife assessment

  This important manuscript used state-of-the-art techniques and employed relevant animal models to provide both convincing and solid evidence supporting the regulatory role of microRNA cluster 221/222 in rheumatoid arthritis synovial fibroblast. The findings of this work offer significant advances to current knowledge which will be interesting to a wide range audience in the rheumatology and bone research fields. However, whereas models, techniques, and analyses are solid, certain concepts related to the role of immune and bone cells are limited.

Reviewed by eLife

Nucleocytoplasmic transport senses mechanics independently of cell density in cell monolayers

1. Ignasi Granero-Moya
2. Guillaume Belthier
3. Bart Groenen
4. Marc Molina-Jordán
5. Miguel González-Martín
6. Xavier Trepat
7. Jacco van Rheenen
8. Ion Andreu
9. Pere Roca-Cusachs

Reviewed by Review Commons

G6b-B antibody-based cis-acting platelet receptor inhibitors (CAPRIs) as a new family of anti-thrombotic therapeutics

1. Alexandra Mazharian
2. Ophélie Bertin
3. Amrita Sarkar
4. Johanna Augros
5. Alicia Bornert
6. Cécile Loubière
7. Friederike Jönsson
8. Juli Warwicker
9. W Mark Abbott
10. Omer Dushek
11. Caroline Vayne
12. Lubica Rauova
13. Klaus Fütterer
14. Jérôme Rollin
15. Mortimer Poncz
16. Yotis A Senis

Reviewed by preLights

Alstrom syndrome proteins regulate centriolar cartwheel assembly by enabling Plk4-Ana2 amplification loop in Drosophila

1. Marine Brunet
2. Joelle Thomas
3. Jean-Andre Lapart
4. Léo Krüttli
5. Marine Laporte
6. Maria Giovanna Riparbelli
7. Giuliano Callaini
8. Benedicte Durand
9. Veronique Morel

Reviewed by Review Commons