1. Time-resolved proximity proteomics uncovers a membrane tension-sensitive caveolin-1 interactome at the rear of migrating cells

    This article has 4 authors:
    1. Eleanor Martin
    2. Rossana Girardello
    3. Gunnar Dittmar
    4. Alexander Ludwig
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study uses convincing time-resolved proximity proteomics, validated with proximity ligation assays, to provide new insight into mechanical regulation of caveolin-1 complexes that form in migrating cells. Solid follow up experiments reveal a reciprocal relationship between mechanosensitive caveolae and RhoGTPase signalling in migrating cells, but evidence supporting a direct link between the newly identified factors with a specific caveolae subpopulation remains incomplete at this stage.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  2. A MSTNDel73C mutation with FGF5 knockout sheep by CRISPR/Cas9 promotes skeletal muscle myofiber hyperplasia

    This article has 21 authors:
    1. Ming-Ming Chen
    2. Yue Zhao
    3. Kun Yu
    4. Xue-Ling Xu
    5. Xiao-Sheng Zhang
    6. Jin-Long Zhang
    7. Su-Jun Wu
    8. Zhi-Mei Liu
    9. Yi-Ming Yuan
    10. Xiao-Fei Guo
    11. Shi-Yu Qi
    12. Guang Yi
    13. Shu-Qi Wang
    14. Huang-Xiang Li
    15. Ao-Wu Wu
    16. Guo-Shi Liu
    17. Shou-Long Deng
    18. Hong-Bing Han
    19. Feng-Hua Lv
    20. Di Lian
    21. Zheng-Xing Lian
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The authors present a useful analysis of the phenotype of sheep in which the muscle developmental regulator myostatin has been mutated in a FGF5 knockout background. The goal was to produce sheep with a "double-muscled" phenotype, yet the genetically engineered sheep exhibited meat with a smaller cross-sectional area and higher number of muscle fibers. The work extends the extensive body of knowledge already published in this area. The authors provide evidence using in vitro experiments that Fosl1 regulates myogenesis, but the strength of evidence relating to the muscle phenotype and underlying cellular and molecular mechanism remains incomplete.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  3. Arpin deficiency increases actomyosin contractility and vascular permeability

    This article has 12 authors:
    1. Armando Montoya-Garcia
    2. Idaira M Guerrero-Fonseca
    3. Sandra D Chanez-Paredes
    4. Karina B Hernandez-Almaraz
    5. Iliana I Leon-Vega
    6. Angelica Silva-Olivares
    7. Abigail Betanzos
    8. Monica Mondragon-Castelan
    9. Ricardo Mondragon-Flores
    10. Citlaltepetl Salinas-Lara
    11. Hilda Vargas-Robles
    12. Michael Schnoor
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents solid results to demonstrate that arpin is expressed in the endothelium of blood vessels and that its deficiency leads to leaky blood vessels in in vivo and in vitro models. The work does not yet clarify the mechanistic connection between arpin and increased ROCK activity. The study adds some insights to our understanding of the complicated network of proteins that control this process, and it will be useful to individuals within this defined field of study.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  4. Unique ultrastructural organization of human rod photoreceptors

    This article has 9 authors:
    1. Tylor R. Lewis
    2. Natalia V. Klementieva
    3. Sebastien Phan
    4. Carson M. Castillo
    5. Keun-Young Kim
    6. Lauren Y. Cao
    7. Mark H. Ellisman
    8. Vadim Y. Arshavsky
    9. Oleg Alekseev

    Reviewed by Arcadia Science

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
  5. Topological stress triggers persistent DNA lesions in ribosomal DNA with ensuing formation of PML-nucleolar compartment

    This article has 12 authors:
    1. Alexandra Urbancokova
    2. Terezie Hornofova
    3. Josef Novak
    4. Sarka Andrs Salajkova
    5. Sona Stemberkova Hubackova
    6. Alena Uvizl
    7. Tereza Buchtova
    8. Martin Mistrik
    9. Brian McStay
    10. Zdenek Hodny
    11. Jiri Bartek
    12. Pavla Vasicova
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study asks how Promyelocytic leukemia protein (PML) becomes associated with the nucleoli of cells (PML Nucleolar Associations, PNAs) upon various genotoxic stimuli. Using immunostaining analysis with induced DNA double-strand breaks (DSBs) in rDNA repeats, the authors provide solid evidence that PNAs are triggered mostly by the inhibition of topoisomerase and RNA polymerase I, which is augmented by homologous recombination but not by the non-homologous end joining double-strand break repair pathway. The findings have potential implications for a better understanding of how DNA damage in ribosomal DNA is repaired for genome stability. This paper is of interest to researchers in the fields of nuclear structure and DNA repair.

    Reviewed by eLife

    This article has 8 evaluationsAppears in 1 listLatest version Latest activity
  6. Allosteric modulation of the CXCR4:CXCL12 axis by targeting receptor nanoclustering via the TMV-TMVI domain

    This article has 25 authors:
    1. Eva M García-Cuesta
    2. Pablo Martínez
    3. Karthik Selvaraju
    4. Gabriel Ulltjärn
    5. Adrián Miguel Gómez Pozo
    6. Gianluca D'Agostino
    7. Sofia Gardeta
    8. Adriana Quijada-Freire
    9. Patricia Blanco Gabella
    10. Carlos Roca
    11. Daniel del Hoyo
    12. Rodrigo Jiménez-Saiz
    13. Alfonso García-Rubia
    14. Blanca Soler Palacios
    15. Pilar Lucas
    16. Rosa Ayala-Bueno
    17. Noelia Santander Acerete
    18. Yolanda Carrasco
    19. Carlos Oscar Sorzano
    20. Ana Martinez
    21. Nuria E Campillo
    22. Lasse D Jensen
    23. Jose Miguel Rodriguez Frade
    24. César Santiago
    25. Mario Mellado
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This is an important study that describes an elegant modelling driven approach to design of allosteric antagonists for CXCR4 that have a selective effect on receptor nanocluster formation, cell polarisation and chemotaxis, but spare binding of CXCL12 to the receptor and inhibition of adenylate cyclase. This enables selective targeting of processes dependent upon cell polarisation and chemotaxis without impacting signalling effects and may avoid some of the toxicity associated with antagonists that target CXCL12 binding and thus block all CXCR4 signalling. The revised manuscript offers convincing evidence to support the claims. The modelling work is better described and additional data has been presented that better illustrates the unique features of the new antagonist. The in vivo studies in the zebrafish model open a path to studies in mammalian models.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 2 listsLatest version Latest activity
  7. The actomyosin system is essential for the integrity of the endosomal system in bloodstream form Trypanosoma brucei

    This article has 14 authors:
    1. Fabian Link
    2. Sisco Jung
    3. Xenia Malzer
    4. Felix Zierhut
    5. Antonia Konle
    6. Alyssa Borges
    7. Christopher Batters
    8. Monika Weiland
    9. Mara Poellmann
    10. An Binh Nguyen
    11. Johannes Kullmann
    12. Claudia Veigel
    13. Markus Engstler
    14. Brooke Morriswood
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study builds on a previous publication, demonstrating that T. brucei has a continuous endomembrane system, which probably facilitates high rates of endocytosis. Using a range of cutting-edge approaches, the authors present compelling evidence that an actomyosin system, with the myosin TbMyo1 as an active molecular motor, is localized close to and can associate with the endosomal system in the bloodstream form of Trypanosoma brucei. It shows convincingly that both actin and Myo I play a role in the organization and integrity of the endosomal system: both RNAi-mediated depletion of Myo1, and treatment of the cells with latrunculin A resulted in endomembrane disruption. This work should be of interest to cell biologists and microbiologists working on the cytoskeleton, and unicellular eukaryotes.

    Reviewed by eLife, Arcadia Science

    This article has 10 evaluationsAppears in 2 listsLatest version Latest activity
  8. Epigenetic insights into GABAergic development in Dravet Syndrome iPSC and therapeutic implications

    This article has 7 authors:
    1. Jens Schuster
    2. Xi Lu
    3. Yonglong Dang
    4. Joakim Klar
    5. Amelie Wenz
    6. Niklas Dahl
    7. Xingqi Chen
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This is a potentially useful study that shows changes in the chromatin landscape of GABAergic neurons in induced pluripotent stem cells (iPSCs) derived from both Dravet Syndrome (DS) patients and healthy donors. The strength of the evidence is currently incomplete because the authors compared iPSCs from different individuals, rather than isogenic controls. A strategy for minimizing variability across cell lines is used, but the explanation is not complete. The revised manuscript adds RNAseq and qPCR measurements of the expression of the gene SCN1A, however these do not appear to agree, perhaps because of the way the qPCR measurements are normalized, and there is no measurement of Nav1.1, the gene product thought to be responsible for the majority of DS cases. Hence the evidence that there is reduced expression of SCN1A or its gene product is not complete and therefore it is difficult to evaluate whether or not the observed epigenetic changes are causal. The work would potentially be of interest to scientists who study development, developmental disorders, and epigenetic contributions to disease.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  9. Detection of TurboID fusion proteins by fluorescent streptavidin outcompetes antibody signals and visualises targets not accessible to antibodies

    This article has 6 authors:
    1. Johanna Odenwald
    2. Bernardo Gabiatti
    3. Silke Braune
    4. Siqi Shen
    5. Martin Zoltner
    6. Susanne Kramer
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study demonstrates how proximity labeling with streptavidin can be used to boost fluorescence signals in otherwise hard-to-label regions of cells. The experimental verification of amplification of fluorescence near epitope tags in phase-separated compartments is solid, demonstrating enhanced signal-to-noise compared to immunofluorescence. This study will be of particular interest to those using correlative light and electron microscopy or expansion microscopy when the signal is limiting or inaccessible.

    Reviewed by eLife

    This article has 8 evaluationsAppears in 1 listLatest version Latest activity
  10. Membrane Curvature Promotes ER-PM Contact Formation via Junctophilin-EHD Interactions

    This article has 14 authors:
    1. Yang Yang
    2. Luis A. Valencia
    3. Chih-Hao Lu
    4. Melissa L. Nakamoto
    5. Ching-Ting Tsai
    6. Chun Liu
    7. Huaxiao Yang
    8. Wei Zhang
    9. Zeinab Jahed
    10. Wan-Ru Lee
    11. Francesca Santoro
    12. Jen Liou
    13. Joseph C. Wu
    14. Bianxiao Cui

    Reviewed by preLights

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
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