Cell Biology

High-content high-resolution microscopy and deep learning assisted analysis reveals host and bacterial heterogeneity during Shigella infection

1. Ana T. López-Jiménez
2. Dominik Brokatzky
3. Kamla Pillay
4. Tyrese Williams
5. Gizem Özbaykal Güler
6. Serge Mostowy

• Curated by eLife

  eLife assessment

  This manuscript describes an AI-automated microscopy-based approach to characterize both bacterial and host cell responses associated with Shigella infection of epithelial cells. The methodology is compelling and should be helpful for investigators studying a variety of intracellular pathogens. The authors have acquired valuable findings regarding host and bacterial responses in the context of infection, which should be followed up with further mechanistic-based studies.

Reviewed by eLife

PILS-Nir1 is a novel phosphatidic acid biosensor that reveals mechanisms of lipid production

1. Claire C Weckerly
2. Taylor A Rahn
3. Max Ehrlich
4. Rachel C Wills
5. Joshua G Pemberton
6. Michael V Airola
7. Gerald R Hammond

Reviewed by Review Commons

Vacuolar H+-ATPase determines daughter cell fates through asymmetric segregation of the nucleosome remodeling and deacetylase complex

1. Zhongyun Xie
2. Yongping Chai
3. Zhiwen Zhu
4. Zijie Shen
5. Zhengyang Guo
6. Zhiguang Zhao
7. Long Xiao
8. Zhuo Du
9. Guangshuo Ou
10. Wei Li

• Curated by eLife

  eLife assessment

  The authors make the intriguing proposal that the NuRD complex in C. elegans, which has been linked to regulation of the cell death protein EGL-1 before, becomes asymmetrically distributed after cell division and that this asymmetry relies on V-ATPase activity. Whereas some disagreement remained between the reviewers' and the authors' interpretation, the final version incorporated alternative possibilities in the text, and with careful interpretation, the current manuscript's model is supported by solid data, and represents a valuable contribution to the field.

Reviewed by eLife

Growth-dependent concentration gradient of the oscillating Min system in Escherichia coli

1. Claudia Parada
2. Ching-Cher Sanders Yan
3. Cheng-Yu Hung
4. I-Ping Tu
5. Chao-Ping Hsu
6. Yu-Ling Shih

Reviewed by Review Commons

Time-resolved interactome profiling deconvolutes secretory protein quality control dynamics

1. Madison T Wright
2. Bibek Timalsina
3. Valeria Garcia Lopez
4. Jake N Hermanson
5. Sarah Garcia
6. Lars Plate

Reviewed by Review Commons

Differential regulation by CD47 and thrombospondin-1 of extramedullary erythropoiesis in mouse spleen

1. Rajdeep Banerjee
2. Thomas J Meyer
3. Margaret C Cam
4. Sukhbir Kaur
5. David D Roberts

• Curated by eLife

  eLife assessment

  This study presents a valuable finding on the cell composition in mouse spleen depleted for the CD47 receptor and its signaling ligand Thrombospondin in hematopoietic differentiation. The supporting evidence is convincing with analytical improvements on the individual contributions of the signaling components and with functional studies. This work has implications for the role of CD47/Thsp in extramedullary erythropoiesis in mouse spleen and will be of interest to medical biologists working on cell signaling, transfusion medicine, and cell therapy.

Reviewed by eLife

A Drug Repurposing Approach Reveals Targetable Epigenetic Pathways in Plasmodium vivax Hypnozoites

1. S. P. Maher
2. M. A. Bakowski
3. A. Vantaux
4. E. L. Flannery
5. C. Andolina
6. M. Gupta
7. Y. Antonova-Koch
8. M. Argomaniz
9. M. Cabrera-Mora
10. B. Campo
11. A. T. Chao
12. A. K. Chatterjee
13. W. T. Cheng
14. E. Chuenchob
15. C. A. Cooper
16. K. Cottier
17. M. R. Galinski
18. A. Harupa-Chung
19. H. Ji
20. S. B. Joseph
21. T. Lenz
22. S. Lonardi
23. J. Matheson
24. S. A. Mikolajczak
25. T. Moeller
26. A. Orban
27. V. Padín-Irizarry
28. K. Pan
29. J. Péneau
30. J. Prudhomme
31. C. Roesch
32. A. A. Ruberto
33. S. S. Sabnis
34. C. L. Saney
35. J. Sattabongkot
36. S. Sereshki
37. S. Suriyakan
38. R. Ubalee
39. Y. Wang
40. P. Wasisakun
41. J. Yin
42. J. Popovici
43. C. W. McNamara
44. C. J. Joyner
45. F. Nosten
46. B. Witkowski
47. K. G. Le Roch
48. D. E. Kyle

• Curated by eLife

  eLife assessment

  This paper reports a large drug repurposing screen based on an in vitro culture platform to identify compounds that can kill Plasmodium hypnozoites. This valuable work adds to the current repertoire of anti-hypnozoites agents and uncovers targetable epigenetic pathways to enhance our understanding of this mysterious stage of the Plasmodium life cycle. The data presented here are based on solid methodology and represent a starting point for further investigation of epigenetic inhibitors to treat P. vivax infection. This paper will be of interest to Plasmodium researchers and more broadly to readers in the fields of host-pathogen interactions and drug development.

Reviewed by eLife

Membrane lipid poly-unsaturation selectively affects ligand induced dopamine D2 receptor internalization

1. Silvia Sposini
2. Rim Baccouch
3. Mathias Lescuyer
4. Véronique De Smedt-Peyrusse
5. Joyce Heuninck
6. Thierry Durroux
7. Pierre Trifilieff
8. David Perrais
9. Isabel Alves

Reviewed by Review Commons

Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects

1. Ritobrata Ghose
2. Fabio Pezzano
3. Savvas Kourtis
4. Ilir Sheraj
5. Shubhamay Das
6. Antoni Gañez Zapater
7. Upamanyu Ghose
8. Lorena Espinar
9. Katja Parapatics
10. Valeria Venturini
11. André C Müller
12. Verena Ruprecht
13. Sara Sdelci

Reviewed by preLights

Neutrophils actively swell to potentiate rapid migration

1. Tamas L Nagy
2. Evelyn Strickland
3. Orion D Weiner

• Curated by eLife

  eLife assessment

  This fundamental study significantly advances our understanding of the role of water influx and swelling on neutrophil migration in response to chemoattractant. The evidence supporting the conclusions, based on a genome-wide CRISPR screen and high quality cellular observations, is compelling. This paper will be of interest to cell biologists and biophysicists working on cell migration.

Reviewed by eLife