Quantitative phase imaging with temporal kinetics predicts hematopoietic stem cell diversity
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Abstract
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between changes in Hlf-tdTomato expression and temporal differentiation in HSCs was confirmed (Fig. 2E,157Fig. S3, F and G). To determine whether Hlf-tdTomato expression levels act as a functional indicator of158HSCs, tdTomato high and low expanded HSCs were transplanted into irradiated recipients, and long-term159bone marrow reconstitution abilities were evaluated. HSCs with high tdTomato demonstrate robust long-160term marrow reconstitution capabilities. Conversely, chimerism decreased in those who received161tdTomato-low HSCs (Fig. 2, F to H). Furthermore, the analysis of the spatiotemporal dynamics of early162hematopoiesis post-transplantation revealed that tdTomato high HSCs gradually reconstituted systemic163hematopoiesis, whereas tdTomato low HSCs induced rapid hematopoiesis between day 14 and 21 (Fig.1642, I to L).
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between changes in Hlf-tdTomato expression and temporal differentiation in HSCs was confirmed (Fig. 2E,157Fig. S3, F and G). To determine whether Hlf-tdTomato expression levels act as a functional indicator of158HSCs, tdTomato high and low expanded HSCs were transplanted into irradiated recipients, and long-term159bone marrow reconstitution abilities were evaluated. HSCs with high tdTomato demonstrate robust long-160term marrow reconstitution capabilities. Conversely, chimerism decreased in those who received161tdTomato-low HSCs (Fig. 2, F to H). Furthermore, the analysis of the spatiotemporal dynamics of early162hematopoiesis post-transplantation revealed that tdTomato high HSCs gradually reconstituted systemic163hematopoiesis, whereas tdTomato low HSCs induced rapid hematopoiesis between day 14 and 21 (Fig.1642, I to L).
This is a really nice demonstration that the levels of Hlf-tdTomato are indicators of 'stemness' but it would also be nice to see a confirmation that the fusion protein is expressed at levels (or not) that are similar to Hlf. Either would be fine given it is a clear indicator of 'stemness' but the molecular correlation would provide clearer ties to the mechanism of 'stemness.'
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