Cell Biology

Single-nucleus transcriptomics reveal the cytological mechanism of conjugated linoleic acids in regulating intramuscular fat deposition

1. Liyi Wang
2. Shiqi Liu
3. Shu Zhang
4. Yizhen Wang
5. Yanbing Zhou
6. Tizhong Shan

• Curated by eLife

  eLife Assessment

  This study provides valuable information on the single nucleus RNA sequencing transcriptome, pathways, and cell types in pig skeletal muscle in response to conjugated linoleic acid (CLA) supplementation. Based on the comprehensive data analyses, the data are considered compelling and provide new insight into the mechanisms underlying intramuscular fat deposition and muscle fiber remodeling. The study contributes significantly to the understanding of nutritional strategies for fat infiltration in pig muscle.

Reviewed by eLife

Med12 cooperates with multiple differentiation signals to facilitate efficient lineage transitions in embryonic stem cells

1. Max Fernkorn
2. Christian Schröter

Reviewed by Review Commons

Piezo1 balances membrane tension and cortical contractility to stabilize intercellular junctions and maintain epithelial barrier integrity

1. Ahsan Javed
2. Aki Stubb
3. Clémentine Villeneuve
4. Franziska Peters
5. Matthias Rübsam
6. Carien M. Niessen
7. Leah C. Biggs
8. Sara A. Wickström

Reviewed by Review Commons

Control of ciliary transcriptional programs during spermatogenesis by antagonistic transcription factors

1. Weihua Wang
2. Junqiao Xing
3. Xiqi Zhang
4. Hongni Liu
5. Xingyu Liu
6. Haochen Jiang
7. Cheng Xu
8. Xue Zhao
9. Zhangfeng Hu

• Curated by eLife

  eLife Assessment

  This valuable study presents data suggesting the critical roles of two ancient proteins, XAP5 and XAP5L, in regulating the transcriptional program of ciliogenesis during mouse spermatogenesis. The supporting data are solid, and this work will be of interest to biomedical researchers studying ciliogenesis and reproduction.

Reviewed by eLife

Multi-omics analyses and machine learning prediction of oviductal responses in the presence of gametes and embryos

1. Ryan M Finnerty
2. Daniel J Carulli
3. Akshata Hedge
4. Yanli Wang
5. Frimpong Boadu
6. Sarayut Winuthayanon
7. Jianlin Jack Cheng
8. Wipawee Winuthayanon

• Curated by eLife

  eLife Assessment

  This important study reports the transcriptomic and proteomic landscapes of the oviducts at four different preimplantation stages during natural fertilization, pseudopregnancy, and superovulation. The supporting data are convincing. This work will be of interest to reproductive biologists and clinicians practicing reproductive medicine.

Reviewed by eLife

Quantification of Cyclin-CDK dissociation constants in living cells using fluorescence cross-correlation spectroscopy with green and near-infrared fluorescent proteins

1. Aika Toyama
2. Yuhei Goto
3. Yuhei Yamauchi
4. Hironori Sugiyama
5. Yohei Kondo
6. Atsushi Mochizuki
7. Kazuhiro Aoki

Reviewed by Review Commons

Bridging the Gap in Cancer Cell Behavior Against Matrix Stiffening: Insights from a Trizonal Model

1. Mohammad E Torki
2. Fan Liu
3. Rongguang Xu
4. Yunfeng Chen
5. Jeffery Fredberg
6. Zi Chen

• Curated by eLife

  eLife Assessment

  This study presents a valuable nonlinear mathematical model that addresses how cell shape transitions in response to ECM stiffness. The evidence supporting the claims of the authors is solid, although additional work is required to improve the manuscript. For instance, the authors should improve the overall readability of the text and amend the experimental validation section. The work will be of interest to scientists working on a spectrum of fields including cell mechanics, cell behaviors, and cancer research.

Reviewed by eLife

Fis1 is required for the development of the dendritic mitochondrial network in pyramidal cortical neurons

1. Klaudia Strucinska
2. Parker Kneis
3. Travis Pennington
4. Katarzyna Cizio
5. Patrycja Szybowska
6. Abigail Morgan
7. Joshua Weertman
8. Tommy L Lewis

Reviewed by preLights

SRS microscopy identifies inhibition of vitellogenesis as a mediator of lifespan extension by caloric restriction in C. elegans

1. Bowen Yang
2. Bryce Manifold
3. Wuji Han
4. Catherin DeSousa
5. Wanyi Zhu
6. Aaron Streets
7. Denis V. Titov

Reviewed by Arcadia Science

MuSpAn: A Toolbox for Multiscale Spatial Analysis

1. Joshua A. Bull
2. Joshua W. Moore
3. Shania M. Corry
4. Muyang Lin
5. Hayley L. Belnoue-Davis
6. Eoghan J. Mulholland-Illingworth
7. Simon J. Leedham
8. Helen M. Byrne

Reviewed by Arcadia Science