Cell Biology

TET2-mediated epigenetic modification promotes stress senescence of pancreatic β cells in type 2 diabetes mellitus

1. Weijuan Cai
2. Qingqing Song
3. Xiaoqing Mo
4. Huaqian Li
5. Yuling Song
6. Liang Yin

• Curated by eLife

  eLife Assessment

  This is a useful study that seeks to address the role of the TET family of DNA demethylation enzymes in pancreatic beta cell senescence in the context of type 2 diabetes (T2DM). Although the concepts are novel and of interest, the study presents incomplete evidence to fully support its main conclusions.

Reviewed by eLife

Loss of ErbB3 redirects Integrin β1 from early endosomal recycling to secretion in extracellular vesicles

1. Dorival Mendes Rodrigues-Junior
2. Ana Rosa Sáez-Ibáñez
3. Takeshi Terabayashi
4. Nina Daubel
5. Taija Mäkinen
6. Olof Idevall-Hagren
7. Aristidis Moustakas
8. Ingvar Ferby

Reviewed by Review Commons

Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages

1. Tomoharu Kanie
2. Roy Ng
3. Keene L Abbott
4. Niaj Mohammad Tanvir
5. Esben Lorentzen
6. Olaf Pongs
7. Peter K Jackson

• Curated by eLife

  eLife assessment

  The identification of NCS1 as a distal appendage protein that captures preciliary vesicles has important implications for understanding the early steps of ciliary assembly. Furthermore, the work has important implications for the broader understanding of NCS1, which prior to this work was focused on roles in neurotransmission, but now must be considered in a broader context. The investigators used a variety of state-of-the-art methodologies, and the conclusions are convincingly supported by the experimental data. This work will be of interest to cell biologists studying ciliary assembly, human geneticists exploring the pathology of cilia as well as neurobiologists studying NCS1.

Reviewed by eLife

Single-molecule tracking reveals the dynamics of Ipl1 recruitment to the kinetochores and spindles in S. cerevisiae

1. Nitesh Kumar Podh
2. Ayan Das
3. Akriti Kumari
4. Kirti Garg
5. Rashmi Yadav
6. Kirti Kashyap
7. Sahil Islam
8. Anupam Gupta
9. Gunjan Mehta

Reviewed by Review Commons

Pharmacologic activation of integrated stress response kinases inhibits pathologic mitochondrial fragmentation

1. Kelsey R Baron
2. Samantha Oviedo
3. Sophia Krasny
4. Mashiat Zaman
5. Rama Aldakhlallah
6. Prerona Bora
7. Prakhyat Mathur
8. Gerald Pfeffer
9. Michael J Bollong
10. Timothy E Shutt
11. Danielle A Grotjahn
12. R Luke Wiseman

• Curated by eLife

  eLife Assessment

  This important study identifies a new class of small molecules that activate the integrated stress response (ISR) via the kinase HRI. Convincing evidence, including the image analysis pipeline, indicates that two of these compounds promote mitochondrial elongation and protect against mitochondrial fragmentation caused by chemical stress conditions or by genetic alterations. These findings open an avenue for new strategies for mitochondrial dysfunction targeting linked to ISR alterations.

Reviewed by eLife

Catalytic growth in a shared enzyme pool ensures robust control of centrosome size

1. Deb Sankar Banerjee
2. Shiladitya Banerjee

• Curated by eLife

  eLife Assessment

  This valuable work suggests a new physical model of centrosome maturation: a catalytic growth model with a shared enzyme pool. The authors provide compelling evidence to show that the model is able to reproduce various experimental results such as centrosome size scaling with cell size and centrosome growth curves in C. elegans, and that the final centrosome size is more robust to differences in initial centrosome size. While direct experimental support for this theory is currently lacking, the authors propose concrete experiments that could distinguish their shared-enzyme model from previously proposed alternatives.

Reviewed by eLife

Lysine demethylase 4A is a centrosome‐associated protein required for centrosome integrity and genomic stability

1. Pratim Chowdhury
2. Xiaoli Wang
3. Richard I. Han
4. Manga Motrapu
5. Ashley G. Boice
6. Yuya Nakatani
7. Sofia Vargas‐Hernandez
8. Julia F. Love
9. Claude Chew
10. Sandra L. Grimm
11. Dereck Mezquita
12. Frank M. Mason
13. Elisabeth D. Martinez
14. Cristian Coarfa
15. Cheryl L. Walker
16. Anna‐Karin Gustavsson
17. Ruhee Dere

Reviewed by Review Commons

A hierarchical pathway for assembly of the distal appendages that organize primary cilia

1. Tomoharu Kanie
2. Beibei Liu
3. Julia F Love
4. Saxton D Fisher
5. Anna-Karin Gustavsson
6. Peter K Jackson

• Curated by eLife

  eLife assessment

  This important and detailed study presents the most comprehensive view of the functional organization and requirements for a mother centriole's distal appendage in primary cilia assembly published to date. Crispr-knockouts and super-resolution microscopy analysis of the distal appendage proteins provides convincing evidence to support the claims of the authors. This work will be of high value to cell biologists and biophysicists working on the structure and function of the centrosome as well as human geneticists exploring ciliary pathology.

Reviewed by eLife

Necrosulfonamide causes oxidation of PCM1 and impairs ciliogenesis and autophagy

1. Clotilde C.N. Renaud
2. Carolina Alves Nicolau
3. Clément Maghe
4. Kilian Trillet
5. Jane Jardine
6. Sophie Escot
7. Nicolas David
8. Julie Gavard
9. Nicolas Bidère

Reviewed by preLights

AtVPS13M1 is involved in lipid remodeling in low phosphate and is located at the mitochondria surface in plants

1. Sébastien Leterme
2. Catherine Albrieux
3. Sabine Brugière
4. Yohann Couté
5. Julien Dellinger
6. Benjamin Gillet
7. Sandrine Hughes
8. Julie Castet
9. Amélie Bernard
10. David Scheuring
11. Marion Schilling
12. Juliette Jouhet
13. Morgane Michaud

Reviewed by Review Commons