Cell Biology

N-cadherin mechanosensing in ovarian follicles controls oocyte maturation and ovulation

1. Alaknanda Emery
2. Orest W. Blaschuk
3. Thao D. Dinh
4. Tim McPhee
5. Rouven Becker
6. Andrew D. Abell
7. Krzysztof M. Mrozik
8. Andrew C.W. Zannettino
9. Rebecca L Robker
10. Darryl L Russell

• Curated by eLife

  eLife assessment

  The manuscript describes important findings regarding the significance of CHD2 in ovarian folliculogenesis. Overall, the results lead to convincing conclusions, with minimal concerns raised by the reviewers. Both the results and conclusions are well discussed. This work will be of interest to ovarian biologists and physicians working on female fertility.

Reviewed by eLife

Organelle landscape analysis using a multi-parametric particle-based method

1. Yoshitaka Kurikawa
2. Ikuko Koyama-Honda
3. Keiko Igarashi
4. Norito Tamura
5. Seiichi Koike
6. Noboru Mizushima

Reviewed by Review Commons

Calcium transients trigger switch-like discharge of prostaglandin E2 in an extracellular signal-regulated kinase-dependent manner

1. Tetsuya Watabe
2. Shinya Yamahira
3. Kanako Takakura
4. Dean Thumkeo
5. Shuh Narumiya
6. Michiyuki Matsuda
7. Kenta Terai

• Curated by eLife

  eLife assessment

  This important study reports on the dynamics of PKA investigated at the single-cell level in vitro and in epithelia in vivo. Using different fluorescent biosensors and optogenetic actuators, the authors dissect the signaling pathway responsible for PKA waves, finding that PKA activation is a consequence of PGE2 release, which in turn is triggered by calcium pulses, requiring high ERK activity. The evidence supporting the claims is solid. At this stage the work is still partly descriptive in nature, and additional measurements would increase the strength of mechanistic insights and physiological relevance.

Reviewed by eLife

Surface curvature and basal hydraulic stress induce spatial bias in cell extrusion

1. Cheng-Kuang Huang
2. Xianbin Yong
3. David T. She
4. Chwee Teck Lim

• Curated by eLife

  eLife assessment

  This paper presents important findings into the response of epithelial monolayers to the combined effects of surface curvature and hydraulic stress, offering insights into how these cues contribute to epithelial cell extrusion. Most of the evidence is convincing, relying mainly on a combination of imaging-based techniques. This paper is of interest to a broad and growing community of biologists, biophysicists, and engineers interested in cell-geometry interactions.

Reviewed by eLife

Single nuclei transcriptomics reveal the differentiation trajectories of periosteal skeletal/stem progenitor cells in bone regeneration

1. Simon Perrin
2. Maria Ethel
3. Vincent Bretegnier
4. Cassandre Goachet
5. Cécile-Aurore Wotawa
6. Marine Luka
7. Fanny Coulpier
8. Cécile Masson
9. Mickael Ménager
10. Céline Colnot

• Curated by eLife

  eLife assessment

  This important study characterizes various cell populations and describes a developmental trajectory using snRNAseq data, highlighting the cell state transitions including periosteal stem cells during bone repair. However, there was a general consensus that the evidence provided is currently incomplete, necessitating the additional data and a more thorough verification of the conclusions. Despite this, the work provides a helpful resource that will be of broad interest to the bone community.

Reviewed by eLife

ADPKD-Causing Missense Variants in Polycystin-1 Disrupt Cell Surface Localization or Polycystin Channel Function

1. Kotdaji Ha
2. Gabriel B. Loeb
3. Meyeon Park
4. Aide Pinedo
5. Christine Haewon Park
6. Nadav Brandes
7. F. Ritu
8. Chun Jimmie Ye
9. Jeremy F. Reiter
10. Markus Delling

Reviewed by Arcadia Science

Heparan sulfate promotes TRAIL-induced tumor cell apoptosis

1. Yin Luo
2. Huanmeng Hao
3. Zhangjie Wang
4. Chih Yean Ong
5. Robert Dutcher
6. Yongmei Xu
7. Jian Liu
8. Lars C Pedersen
9. Ding Xu

• Curated by eLife

  eLife assessment

  This fundamental study advances our understanding of TRAIL-induced apoptosis by defining how Heparan triggers this pathway at the molecular level. The evidence supporting the conclusions is compelling, with rigorous binding assays, structural methods, and cellular studies. The work will be of broad interest to cell biologists and biochemists.

Reviewed by eLife

Context-dependent modification of PFKFB3 in hematopoietic stem cells promotes anaerobic glycolysis and ensures stress hematopoiesis

1. Shintaro Watanuki
2. Hiroshi Kobayashi
3. Yuki Sugiura
4. Masamichi Yamamoto
5. Daiki Karigane
6. Kohei Shiroshita
7. Yuriko Sorimachi
8. Shinya Fujita
9. Takayuki Morikawa
10. Shuhei Koide
11. Motohiko Oshima
12. Akira Nishiyama
13. Koichi Murakami
14. Miho Haraguchi
15. Shinpei Tamaki
16. Takehiro Yamamoto
17. Tomohiro Yabushita
18. Yosuke Tanaka
19. Go Nagamatsu
20. Hiroaki Honda
21. Shinichiro Okamoto
22. Nobuhito Goda
23. Tomohiko Tamura
24. Ayako Nakamura-Ishizu
25. Makoto Suematsu
26. Atsushi Iwama
27. Toshio Suda
28. Keiyo Takubo

• Curated by eLife

  eLife assessment

  This important study provides novel strategies to overcome certain limitations when investigating the metabolism of hematopoietic stem cells, mainly due to their low abundance. The study provides compelling evidence suggesting that proliferative hematopoietic stem cells mainly use glycolysis (rather than mitochondrial OXPHOS or TCA cycle) as their primary energy source during emergency hematopoiesis. The article provides direct links between metabolic features and cell proliferation and explores alternative energy sources, and is of great interest to stem cell biologists.

Reviewed by eLife

Control of cell retraction and protrusion with a single protein

1. Jean De Seze
2. Maud Bongaerts
3. Benoit Boulevard
4. Mathieu Coppey

• Curated by eLife

  eLife assessment

  This important study combines experiments with optogenetic actuation and theory to understand how signalling proteins control the switch between cell protrusion and retraction, two processes in single-cell migration. The authors examine the role of a guanine exchange factor (GEF) on the downstream effectors RhoA and Cdc42, which trigger retraction and protrusion, respectively. The experimental and theoretical evidence provides a convincing explanation for why and how a single signalling protein – here, a GEF of RhoA – can control both protrusion and retraction.

Reviewed by eLife

Basement membrane diversification relies on two competitive secretory routes defined by Rab10 and Rab8 and modulated by dystrophin and the exocyst complex

1. Cynthia Dennis
2. Pierre Pouchin
3. Graziella Richard
4. Vincent Mirouse

Reviewed by Review Commons