Cell Biology

Genome-wide analysis of anterior-posterior mRNA regionalization in Stentor coeruleus reveals a role for the microtubule cytoskeleton

1. Ashley R. Albright
2. Connie Yan
3. David Angeles-Albores
4. Tatyana Makushok
5. Jamarc Allen-Henderson
6. Wallace F. Marshall

Reviewed by Arcadia Science

Constitutively active receptor ADGRA3 signaling induces adipose thermogenesis

1. Zewei Zhao
2. Longyun Hu
3. Bigui Song
4. Tao Jiang
5. Qian Wu
6. Jiejing Lin
7. Xiaoxiao Li
8. Yi Cai
9. Jin Li
10. Bingxiu Qian
11. Siqi Liu
12. Jilu Lang
13. Zhonghan Yang

• Curated by eLife

  eLife Assessment

  The study highlights adhesion G-protein-coupled receptor A3 (ADGRA3) as a potential target for activating adaptive thermogenesis in both white and brown adipose tissue. This finding offers valuable insights for researchers in the field of adipose tissue biology and metabolism. The authors have presented additional evidence to address the reviewers' comments, including experiments conducted on primary stromal vascular fractions from adipose tissues. However, the revised manuscript fails to address several reviewer concerns, such as the measurement of whole-body energy expenditure through indirect calorimetry and the assessment of food intake. Furthermore, the nanoparticle-mediated knockdown of Adgra3 did not adequately address the tissue selectivity of ADGRA in mice. As a result, the primary claims of the study are only partially supported by the available data, leading to the conclusion that the research is deemed incomplete.

Reviewed by eLife

Impairment of lipid homeostasis causes lysosomal accumulation of endogenous protein aggregates through ESCRT disruption

1. John Yong
2. Jacqueline E Villalta
3. Ngoc Vu
4. Matthew A Kukurugya
5. Niclas Olsson
6. Magdalena Preciado López
7. Julia R Lazzari-Dean
8. Kayley Hake
9. Fiona E McAllister
10. Bryson D Bennett
11. Calvin H Jan

• Curated by eLife

  eLife Assessment

  Protein and lipid homeostasis is essential for maintaining cellular functions but their crosstalk remains largely unknown. This important manuscript deals with this interesting topic and applies the powerful unbiased tools of somatic cell genetics to discover evidence suggesting a link between sphingolipids/cholesterol ester metabolism and lysosomal protein aggregation. The authors provide compelling orthogonal evidence to support their conclusions.

Reviewed by eLife

PEBP1 amplifies mitochondrial dysfunction-induced integrated stress response

1. Ling Cheng
2. Ian Meliala
3. Yidi Kong
4. Jingyuan Chen
5. Christopher G Proud
6. Mikael Björklund

• Curated by eLife

  eLife Assessment

  In this article, Cheng et al present an important finding that advances the understanding of mitochondrial stress response(s). The authors employed mass spectrometry-based methods in conjunction with standard molecular and cellular biology techniques to provide compelling evidence that phosphatidylethanolamine-binding protein 1 (PEBP1) acts as a pivotal regulator of the mitochondrial component of integrated stress response. Notwithstanding that this discovery is likely to be of significant interest to researchers across a broad spectrum of disciplines ranging from cell biology to neuroscience, it was thought that further mechanistic dissection of the role of PEBP1 in modulating integrated stress response may further strengthen this study.

Reviewed by eLife

Single-molecule lipid biosensors mitigate inhibition of endogenous effector proteins

1. Victoria L. Holmes
2. Morgan M.C. Ricci
3. Claire C. Weckerly
4. Michael Worcester
5. Gerald R.V. Hammond

Reviewed by Review Commons

Cytoplasmic ribosomes hitchhike on mitochondria to dendrites

1. Corbin J Renken
2. Susie Kim
3. Youjun Wu
4. Marc Hammarlund
5. Shaul Yogev

Reviewed by PREreview

A novel human pluripotent stem cell gene activation system identifies IGFBP2 as a mediator in the production of haematopoietic progenitors in vitro

1. Paolo Petazzi
2. Telma Ventura
3. Francesca Paola Luongo
4. Heather McClafferty
5. Alisha May
6. Helen Alice Taylor
7. Michael J Shipston
8. Nicola Romanò
9. Lesley M Forrester
10. Pablo Menendez
11. Antonella Fidanza

• Curated by eLife

  eLife Assessment

  This study presents useful findings to inform and improve the in vitro differentiation of hematopoietic progenitor cells from human induced pluripotent stem cells. Relying on a well-characterised technical approach, the data analysis is overall solid and reasonably supports the main conclusions.

Reviewed by eLife

Unbiased identification of cell identity in dense mixed neural cultures

1. Sarah De Beuckeleer
2. Tim Van De Looverbosch
3. Johanna Van Den Daele
4. Peter Ponsaerts
5. Winnok H De Vos

• Curated by eLife

  eLife Assessment

  This study presents an important application of high-content image-based morphological profiling to quantitatively and systematically characterize induced pluripotent stem cell-derived mixed neural cultures cell type compositions. Exceptional evidence through rigorous experimental and computational validations support new potential applications of this cheap and simple assay.

Reviewed by eLife

The dependence of shugoshin on Bub1-kinase activity is dispensable for the maintenance of spindle assembly checkpoint response in Cryptococcus neoformans

1. Satya Dev Polisetty
2. Krishna Bhat
3. Kuladeep Das
4. Ivan Clark
5. Kevin G. Hardwick
6. Kaustuv Sanyal

Reviewed by Review Commons

Immunosuppression Drugs Exhibit Differential Effects on Endothelial Cell Function

1. Aly Elezaby
2. Ryan Dexheimer
3. David Wu
4. Sze Yu Chan
5. Ines Ross Tacco
6. Ian Y. Chen
7. Nazish Sayed
8. Karim Sallam

Reviewed by PREreview