Cell Biology

A systematic bi-genomic split-GFP assay illuminates the mitochondrial matrix proteome and protein targeting routes

1. Yury S Bykov
2. Solène Zuttion
3. Bruno Senger
4. Ofir Klein
5. Yeynit Asraf
6. Hadar Meyer
7. Hubert D Becker
8. Róża Kucharczyk
9. Maya Schuldiner

• Curated by eLife

  eLife assessment

  This study represents a valuable addition to the catalog of mitochondrial proteins. With the use of methodology based on the bi-genomic split-GFP technology, the authors generate convincing data, including dually localized proteins and topological information, under various growth conditions in yeast. The study represents a starting point for further functional and/or mechanistic studies on mitochondrial protein biogenesis.

Reviewed by eLife

The ORP9-ORP11 dimer promotes sphingomyelin synthesis

1. Birol Cabukusta
2. Shalom Borst Pauwels
3. Jimmy JLL Akkermans
4. Niek Blomberg
5. Aat A Mulder
6. Roman I Koning
7. Martin Giera
8. Jacques Neefjes

• Curated by eLife

  eLife assessment

  This valuable manuscript systematically addresses the role of intracellular lipid transfer proteins on cellular lipid levels. It provides convincing evidence on the role of ORP9 and ORP11 in sphingolipid metabolism at the Golgi complex. This article will be of broad interest to cell biologists interested in lipid metabolism and membrane biology.

Reviewed by eLife

Spatial proteomics of ER tubules reveals CLMN, an ER-actin tether at focal adhesions that promotes cell migration

1. Holly Merta
2. Kaitlynn Gov
3. Tadamoto Isogai
4. Blessy Paul
5. Achinta Sannigrahi
6. Arun Radhakrishnan
7. Gaudenz Danuser
8. W. Mike Henne

Reviewed by PREreview

Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy

1. Mitsuhiro Abe
2. Masataka Yanagawa
3. Michio Hiroshima
4. Toshihide Kobayashi
5. Yasushi Sako

Reviewed by Review Commons

Activation of the mitochondrial unfolded protein response regulates the formation of stress granules

1. Marta Lopez-Nieto
2. Zhaozhi Sun
3. Emily Relton
4. Rahme Safakli
5. Brian D. Freibaum
6. J Paul Taylor
7. Alessia Ruggieri
8. Ioannis Smyrnias
9. Nicolas Locker

Reviewed by Review Commons

Katanin, kinesin-13, and ataxin-2 inhibit premature interaction between maternal and paternal genomes in C. elegans zygotes

1. Elizabeth A Beath
2. Cynthia Bailey
3. Meghana Mahantesh Magadam
4. Shuyan Qiu
5. Karen L McNally
6. Francis J McNally

• Curated by eLife

  eLife assessment

  This is a valuable paper that identifies a potential challenge for embryos during fertilization: holding sperm contents in the fertilized embryos away from the oocyte meiotic spindle so that they don't get ejected into the polar body during meiotic chromosome segregation. The authors identify proteins involved in cytoplasmic streaming and maintaining the grouping of paternal organelles as being critical for this process. There remain minor weaknesses in the data presented but the paper provides solid evidence for the majority of its claims, and while the findings may pertain to a narrow audience the tools used and basic characterization shown will likely be relied upon by many in the community and therefore is of high value.

Reviewed by eLife

Human receptive endometrial assembloid for deciphering the implantation window

1. Yu Zhang
2. Rusong Zhao
3. Chaoyan Yang
4. Jinzhu Song
5. Peishu Liu
6. Yan Li
7. Boyang Liu
8. Tao Li
9. Changjian Yin
10. Minghui Lu
11. Zhenzhen Hou
12. Chuanxin Zhang
13. Zi-Jiang Chen
14. Keliang Wu
15. Han Zhao

• Curated by eLife

  eLife assessment

  This study presents a valuable development of endometrial organoid culture methodology that mimics the window of implantation. Functional validation to demonstrate its robustness is lacking; therefore, the study is considered incomplete. The data may be interesting to embryologists and investigators working on reproductive biology and medicine.

Reviewed by eLife

Mapping the human hematopoietic stem and progenitor cell hierarchy through integrated single-cell proteomics and transcriptomics

1. Benjamin Furtwängler
2. Nil Üresin
3. Sabrina Richter
4. Mikkel Bruhn Schuster
5. Despoina Barmpouri
6. Henrietta Holze
7. Anne Wenzel
8. Kirsten Grønbæk
9. Kim Theilgaard-Mönch
10. Fabian J. Theis
11. Erwin M. Schoof
12. Bo T Porse

Reviewed by Arcadia Science

Coherent Raman microscopy detects nucleolar defects through amide I peak shifts originating from β-sheets: an application to visualizing ongoing cellular senescence

1. Shigeo Ishibashi
2. Akihito Inoko
3. Yuki Oka
4. Philippe Leproux
5. Hideaki Kano

Reviewed by Arcadia Science

Cell-cycle-dependent mRNA localization in P-bodies

1. Adham Safieddine
2. Marie-Noëlle Benassy
3. Thomas Bonte
4. Floric Slimani
5. Oriane Pourcelot
6. Michel Kress
7. Michèle Ernoult-Lange
8. Maïté Courel
9. Emeline Coleno
10. Arthur Imbert
11. Antoine Laine
12. Annie Munier Godebert
13. Angelique Vinit
14. Corinne Blugeon
15. Guillaume Chevreux
16. Daniel Gautheret
17. Thomas Walter
18. Edouard Bertrand
19. Marianne Bénard
20. Dominique Weil

Reviewed by preLights