Cell Biology

Disease-associated programming of cell memory in glycogen storage disorder type 1a

1. U Sprecher
2. J D’Souza
3. K Mishra
4. N Muchtar
5. O Shalev
6. A Eliassaf
7. A Morshina
8. A Canella Miliano
9. G Mithieux
10. F Rajas
11. S Avraham
12. F Castellani Moses
13. H Kauffman
14. Y Bergman
15. N Garti
16. S Garti
17. M Linial
18. Y Anikster
19. O Kakhlon
20. M Weil

Reviewed by Review Commons

NFAT2 drives both Orai3 transcription and protein degradation by harnessing the differences in epigenetic landscape

1. Sharon Raju
2. Akshay Sharma
3. Gyan Ranjan
4. Rajender K Motiani

Reviewed by Review Commons

Differential spatial regulation and activation of integrin nanoclusters inside focal adhesions

1. Sarah Keary
2. Nicolas Mateos
3. Felix Campelo
4. Maria F Garcia-Parajo

• Curated by eLife

  eLife Assessment

  In this manuscript, the authors analyse the nanoscale localisation of α5β1 and αVβ3 integrins in integrin adhesion complexes (IAC) by dual-colour STORM and assess the spatial organisation at the nano and mesoscale of their main adaptors (paxillin, talin and vinculin). This is an important work that provides detailed analyses that reveal how elements of these complex structures are really organised at the nanoscale, an essential perspective for a better understanding of how IACs function and regulate mechanotransduction processes. The evidence presented is solid, with super-resolution imaging experiments conducted using a single, validated methodology and subsequent computational modelling that enabled a quantitative assessment of the resulting data.

Reviewed by eLife

Nim1-related kinases regulate septin organization and cytokinesis by modulating Hof1 at the cell division site

1. Bindu Bhojappa
2. Anubhav Dhar
3. Bagyashree VT
4. Jayanti Kumari
5. Freya Cardozo
6. Vaseef Rizvi
7. Saravanan Palani

• Curated by eLife

  eLife Assessment

  This valuable study determines the functional requirements for localization and activity of S. cerevisiae septin-associated kinases using in vivo imaging, in vitro and in vivo protein-protein interaction assays, and an instructive in vivo "tethering" approach. In addition to confirming previous results, the study offers evidence that the septin-associated kinases may directly interact with the contractile ring machinery. Although the experiments appear to have been conducted correctly, the quantitative analysis of some experiments is incomplete and should be improved to strengthen the conclusions.

Reviewed by eLife

Glutamine catabolism supports amino acid biosynthesis and suppresses the integrated stress response to promote photoreceptor survival

1. Moloy T Goswami
2. Eric Weh
3. Shubha Subramanya
4. Katherine M Weh
5. Hima Bindu Durumutla
6. Heather Hager
7. Nicholas Miller
8. Sraboni Chaudhury
9. Anthony Andren
10. Peter Sajjakulnukit
11. Li Zhang
12. Cagri Besirli
13. Costas A Lyssiotis
14. Thomas J Wubben

• Curated by eLife

  eLife Assessment

  Goswami and colleagues used rod-specific Gls1 (the gene encoding glutaminase 1) knockout mice to investigate the role of GLS1 in photoreceptor health when GLS1 was deleted from developing or adult photoreceptor cells. This study is fundamental as it shows the critical role of glutamine catabolism in photoreceptor cell health using in vivo model systems. The evidence supporting the authors' claims is compelling. The studies add new insight into how specific metabolites support vision.

Reviewed by eLife

CellSeg3D, Self-supervised 3D cell segmentation for fluorescence microscopy

1. Cyril Achard
2. Timokleia Kousi
3. Markus Frey
4. Maxime Vidal
5. Yves Paychere
6. Colin Hofmann
7. Asim Iqbal
8. Sebastien B Hausmann
9. Stéphane Pagès
10. Mackenzie Weygandt Mathis

• Curated by eLife

  eLife Assessment

  This important work presents a self-supervised method for the segmentation of 3D cells in fluorescent microscopy images, conveniently packaged as a Napari plugin and tested on an annotated dataset. The segmentation method is solid and compares favorably to other learning-based methods and Otsu thresholding on four datasets, offering the possibility of eliminating time-consuming data labeling to speed up quantitative analysis. This work will be of interest to a wide variety of laboratories analysing fluorescently labeled images.

Reviewed by eLife

SDS-22 stabilizes the PP1 catalytic subunits GSP-1/-2 contributing to polarity establishment in C. elegans embryos

1. Yi Li
2. Ida Calvi
3. Monica Gotta

Reviewed by Review Commons

Disruption of the novel nested gene Aff3ir mediates disturbed flow-induced atherosclerosis in mice

1. Shuo He
2. Lei Huang
3. Zhuozheng Chen
4. Ze Yuan
5. Yue Zhao
6. Lingfang Zeng
7. Yi Zhu
8. Jinlong He

• Curated by eLife

  eLife Assessment

  The study presents valuable findings on the role of Aff3ir, a gene implicated in flow-induced atherosclerosis and regulating the inflammation-associated transcription factor, IRF5. The in vivo data are solid in providing evidence on the role of Aff3ir in shear stress and formation of atheromatous plaques. The work will be of interest to clinical researchers and biologists focusing on inflammation and atherosclerosis in cardiovascular disease with a broad eLife readership.

Reviewed by eLife

Molecular mapping and functional validation of GLP-1R cholesterol binding sites in pancreatic beta cells

1. Affiong Ika Oqua
2. Kin Chao
3. Liliane El Eid
4. Lisa Casteller
5. Billy P Baxter
6. Alba Miguéns-Gómez
7. Sebastian Barg
8. Ben Jones
9. Jorge Bernardino de la Serna
10. Sarah L Rouse
11. Alejandra Tomas

• Curated by eLife

  eLife Assessment

  The study presents a valuable finding on the role of cholesterol-binding sites on GLP-1 receptors although the clinical ramifications are unclear and not eminent at this point. Based on the detailed and persuasive responses provided by authors to the concerns raised by reviewers, the revised manuscript is improved substantially and is convincing enough in its scientific merit. The study is a good addition to the scientific community working on receptor biology and drug development for GLP-1 R.

Reviewed by eLife

HNF4α-TET2-FBP1 axis contributes to gluconeogenesis and type 2 diabetes

1. Hongchen Li
2. Xinchao Zhang
3. Xiaoben Liang
4. Shuyan Li
5. Ziyi Cui
6. Xinyu Zhao
7. Kai Wang
8. Bingbing Zha
9. Haijie Ma
10. Ming Xu
11. Lei Lv
12. Yanping Xu

• Curated by eLife

  eLife Assessment

  Zhang et al. present important findings that reveal a new role for TET2 in controlling glucose production in the liver, showing that both fasting and a high-fat diet increase TET2 levels, while its absence reduces glucose production. TET2 works with HNF4α to activate the FBP1 gene upon glucagon stimulation, while metformin disrupts TET2-HNF4α interaction, lowering FBP1 levels and improving glucose homeostasis. The results are convincing and expand our understanding of gluconeogenesis regulation.

Reviewed by eLife