Cell Biology

Multi-species genome-wide CRISPR screens identify conserved suppressors of cold-induced cell death

1. Breanna Lam
2. Kathrin M. Kajderowicz
3. Heather R. Keys
4. Julian M. Roessler
5. Evgeni M. Frenkel
6. Adina Kirkland
7. Punam Bisht
8. Mohamed A. El-Brolosy
9. Rudolf Jaenisch
10. George W. Bell
11. Jonathan S. Weissman
12. Eric C. Griffith
13. Sinisa Hrvatin

• Curated by eLife

  eLife Assessment

  This study presents important findings on cold tolerance shared between hibernating and non-hibernating mammals, identifying a key molecule, GPX4, through multi-species genome-wide CRISPR screens. The evidence supporting these conclusions is compelling, combining multi-species CRISPR screening with rigorous pharmacological assays. This work will be of significant interest to biologists studying hibernation physiology and medical researchers interested in cold tolerance.

Reviewed by eLife

MDC1 mediates Pellino recruitment to sites of DNA double-strand breaks

1. Mònica Torres Esteban
2. Matthew J Stewart
3. Eilis Bragginton
4. Alice Meroni
5. Annica Pellizzari
6. Alain Jeanrenaud
7. Stephen J Smerdon
8. Manuel Stucki

Reviewed by Review Commons

miRNA-29-CLIP uncovers new targets and functions to improve skin repair

1. Lalitha Thiagarajan
2. Rosa Sanchez-Alvarez
3. Chiho Kambara
4. Poojitha Rajasekar
5. Yuluang Wang
6. Francois Halloy
7. Jonathan Hall
8. Hans-Juergen Stark
9. Iris Martin
10. Petra Boukamp
11. Svitlana Kurinna

Reviewed by Review Commons

Osterix Facilitates Osteocytic Communication by Targeting Connexin43

1. Zuping Wu
2. Qian Chen
3. Qian Gao
4. Muchun Liang
5. Yumeng Zhou
6. Li Zhu
7. Jiahe Wang
8. Yang Shen
9. Junjun Jing
10. Jing Xie
11. Xiaoheng Liu
12. Shujuan Zou
13. Demao Zhang
14. Chenchen Zhou

• Curated by eLife

  eLife Assessment

  This study offers valuable insights into the molecular mechanisms by which Osx influences osteocyte function, particularly through its regulation of Cx43. However, the evidence supporting the authors' claims is incomplete, necessitating additional experimental data and further investigation to fully substantiate these findings. While this study presents a new perspective on the complex role of Osx in bone biology, it also raises significant questions about the intricacies of its regulatory network.

Reviewed by eLife

The Rab7-Epg5 and Rab39-ema modules cooperatively position autophagosomes for efficient lysosomal fusions

1. Attila Boda
2. Villő Balázs
3. Anikó Nagy
4. Dávid Hargitai
5. Mónika Lippai
6. Zsófia Simon-Vecsei
7. Márton Molnár
8. Fanni Fürstenhoffer
9. Gábor Juhász
10. Péter Lőrincz

• Curated by eLife

  eLife Assessment

  This paper presents valuable findings on how autophagosomes are positioned along microtubules for their efficient fusion with lysosomes, providing significant insights into the mechanism. The evidence supporting the conclusions is solid, with high-quality fluorescence microscopy combined with Drosophila genetics. This work will be of broad interest to cell biologists interested in autophagy and related cell biology fields.

Reviewed by eLife

Full-length direct RNA sequencing uncovers stress granule-dependent RNA decay upon cellular stress

1. Showkat Ahmad Dar
2. Sulochan Malla
3. Vlastimil Martinek
4. Matthew John Payea
5. Christopher Tai-Yi Lee
6. Jessica Martin
7. Aditya Jignesh Khandeshi
8. Jennifer L Martindale
9. Cedric Belair
10. Manolis Maragkakis

• Curated by eLife

  eLife Assessment

  This important study describes mRNA shortening during cellular stress and interestingly observes that this shortening is dependent on localization in stress granules. Surprisingly, this mRNA shortening does not appear to require the shortening of poly A tails. These are novel, paradigm-shifting findings, using cutting-edge technologies and convincing data, that should be of broad interest to the RNA community and beyond.

Reviewed by eLife, PREreview

MED26-enriched condensates drive erythropoiesis through modulating transcription pausing

1. Shicong Zhu
2. Xiaoting Zhang
3. Na Li
4. Xinying Zhao
5. Man Li
6. Si Xie
7. Qiuyu Yue
8. Yunfeng Li
9. Dong Li
10. Fan Wu
11. Zile Zhang
12. Ziqi Feng
13. Yiyang Zhang
14. Wonhyung Choi
15. Xinyi Jia
16. Yuelin Deng
17. Qi Hu
18. Xingyun Yao
19. Xiaofei Gao
20. Hsiang-Ying Lee

• Curated by eLife

  eLife Assessment

  The study is important to show the role of MED26 in red cell formation. Linking transcription pausing with erythropoiesis is a key discovery. The data are solid although there are still spaces to improve. The in vivo data are limited by specificity concerns on their Cre model. Having RNA-seq, using more erythroid markers such as band3 and a4-integrin, and orthogonal validation with iPSC-erythropoiesis model will improve the study.

Reviewed by eLife

Dynamic tracking of native precursors in adult mice

1. Suying Liu
2. Sarah E Adams
3. Haotian Zheng
4. Juliana Ehnot
5. Seul K Jung
6. Greer Jeffrey
7. Theresa Menna
8. Louise Purton
9. Hongzhe Lee
10. Peter Kurre

• Curated by eLife

  eLife Assessment

  This important study by Liu and colleagues uses lineage tracing of hematopoietic stem and progenitor cells in situ to infer the clonal dynamics of adult hematopoiesis. The authors apply a new mathematical analysis framework enabling a wider range of clonal estimation and the revised study 1) provides evidence of polyclonal adult hematopoiesis, 2) provides insights on clonal dynamics during fetal liver hematopoiesis, and 3) reveals unexpectedly high polyclonality in a mouse model of bone marrow failure (Fanconi anemia), arguing against the prevalent views of clonal attrition in this context. The evidence in this extensively revised and improved study is compelling, with methods, data and analyses more rigorous than the current state-of-the-art, which will be of broad interest not only to stem cell and developmental biologists working on hematopoiesis, but also to researchers working on other systems.

Reviewed by eLife

Amoeboid cells undergo durotaxis with soft end polarized NMIIA

1. Chenlu Kang
2. Pengcheng Chen
3. Xin Yi
4. Dong Li
5. Yiping Hu
6. Yihong Yang
7. Huaqing Cai
8. Bo Li
9. Congying Wu

• Curated by eLife

  eLife Assessment

  This study presents an important finding on durotaxis in various amoeboid cells that is independent of focal adhesions. The evidence supporting the authors' claims is compelling. The work will be of interest to cell biologists and biophysicists working on rigidity sensing, the cytoskeleton, and cell migration.

Reviewed by eLife

UGGT1-mediated reglucosylation of N-glycan competes with ER-associated degradation of unstable and misfolded glycoproteins

1. Satoshi Ninagawa
2. Masaki Matsuo
3. Deng Ying
4. Shuichiro Oshita
5. Shinya Aso
6. Kazutoshi Matsushita
7. Mai Taniguchi
8. Akane Fueki
9. Moe Yamashiro
10. Kaoru Sugasawa
11. Shunsuke Saito
12. Koshi Imami
13. Yasuhiko Kizuka
14. Tetsushi Sakuma
15. Takashi Yamamoto
16. Hirokazu Yagi
17. Koichi Kato
18. Kazutoshi Mori

• Curated by eLife

  eLife Assessment

  This important manuscript demonstrates that UGGT1 is involved in preventing the premature degradation of endoplasmic reticulum (ER) glycoproteins through the re-glucosylation of their N-linked glycans following release from the calnexin/calreticulin lectins. The authors include a wealth of convincing data in support of their findings, although extending these findings to other types of substrates, such as secreted proteins, could further demonstrate the global importance of this mechanism for protein trafficking through the secretory pathway. This work will be of interest to scientists interested in ER protein quality control, proteostasis, and protein trafficking.

Reviewed by eLife