Cell Biology

Oversized cells activate global proteasome-mediated protein degradation to maintain cell size homeostasis

1. Shixuan Liu
2. Ceryl Tan
3. Chloe Melo-Gavin
4. Miriam B Ginzberg
5. Ron Blutrich
6. Nish Patel
7. Michael Rape
8. Kevin G Mark
9. Ran Kafri

• Curated by eLife

  Evaluation Summary:

  Kafri and colleagues assess the contribution of protein degradation to the cell size-dependent accumulation of total protein. As cells get too big, the efficiency of cell growth decreases, which the authors propose is due to increased protein degradation in larger cells. This is an interesting and novel mechanism, and its discovery is potentially useful for future research on understanding and controlling cell growth, though the data could be further strengthened and clarified to support the conclusions.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

The role of ER exit sites in maintaining P-body organization and transmitting ER stress response during Drosophila melanogaster oogenesis

1. Samantha N. Milano
2. Livia V. Bayer
3. Julie J. Ko
4. Caroline E. Casella
5. Diana P. Bratu

Reviewed by preLights

Septins function in exocytosis via physical interactions with the exocyst complex in fission yeast cytokinesis

1. Davinder Singh
2. Yajun Liu
3. Yi-Hua Zhu
4. Sha Zhang
5. Shelby Naegele
6. Jian-Qiu Wu

• Curated by eLife

  eLife assessment

  How secretion is regulated during cell division and how membrane trafficking factors cooperate with the cytoskeleton during cell division remain poorly understood. In this work the authors find potential direct interactions between the polymeric septin cytoskeleton and the exocyst complex, using fission yeast as a model organism. The work provides a valuable body of new information that will be of great interest to the cell biology community. The evidence is strong and rigorous in many places but is incomplete in other respects.

Reviewed by eLife

Hexokinase regulates Mondo-mediated longevity via the PPP and organellar dynamics

1. Raymond Laboy
2. Marjana Ndoci
3. Maximilian Vonolfen
4. Eugen Ballhysa
5. Shamsh Tabrez Syed
6. Tim Droth
7. Klara Schilling
8. Anna Löhrke
9. Ilian Atanassov
10. Adam Antebi

• Curated by eLife

  eLife assessment

  This important study utilizes the nematode C. elegans and mammalian cell culture to investigate the role of MML-1/Mondo in conserved regulation of metabolism and aging. The evidence supporting the conclusions is convincing and covers a range of areas including localization, upstream pathways, and conservation. The paper will be of interest to a broad range of biologists studying aging, metabolism, and transcriptional regulation.

Reviewed by eLife

Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization

1. Martina Jabloñski
2. Guillermina M Luque
3. Matias Gomez Elias
4. Claudia Sanchez Cardenas
5. Xinran Xu
6. Jose L de La Vega Beltran
7. Gabriel Corkidi
8. Alejandro Linares
9. Victor Abonza
10. Aquetzalli Arenas-Hernandez
11. María DP Ramos-Godinez
12. Alejandro López-Saavedra
13. Dario Krapf
14. Diego Krapf
15. Alberto Darszon
16. Adán Guerrero
17. Mariano G Buffone

• Curated by eLife

  eLife assessment

  This important work substantially advances our understanding of sperm motility regulation during fertilization process by uncovering the midpiece/mitochondria contraction associated with motility cessation and structural changes in the midpiece actin network as its mode of action involved. The evidence supporting the conclusion is solid, with rigorous live cell imaging using state-of-art microscopy, although more functional analysis of the midpiece/mitochondria contraction would have further strengthened the study. The work will be of broad interest to cell biologists working on the cytoskeleton, mitochondria, cell fusion, and fertilization.

Reviewed by eLife

A Ctnnb1 enhancer transcriptionally regulates Wnt signaling dosage to balance homeostasis and tumorigenesis of intestinal epithelia

1. Xiaojiao Hua
2. Chen Zhao
3. Jianbo Tian
4. Junbao Wang
5. Xiaoping Miao
6. Gen Zheng
7. Min Wu
8. Mei Ye
9. Ying Liu
10. Yan Zhou

• Curated by eLife

  eLife assessment

  Ctnnb1 encodes β-catenin, an essential component of the canonical Wnt signaling pathway. In this important study, the authors identify an upstream enhancer of Ctnnb1 responsible for the specific expression level of β-catenin in the gastrointestinal track. Deletion of this enhancer in mice and analyses of its association with human colorectal tumors provide compelling support that it controls the dosage of Wnt signaling critical to the homeostasis in intestinal epithelia and colorectal cancers.

Reviewed by eLife

Significantly reduced, but balanced, rates of mitochondrial fission and fusion are sufficient to maintain the integrity of yeast mitochondrial DNA

1. Brett T. Wisniewski
2. Laura L. Lackner

Reviewed by preLights

STAG3 promotes exit from pluripotency through post-transcriptional mRNA regulation in the cytoplasm

1. Sam Weeks
2. Dubravka Pezic
3. Martin Dodel
4. Kunal Shah
5. Amandeep Bhamra
6. Stephen Henderson
7. Silvia Surinova
8. Tyson Sharp
9. Faraz Mardakheh
10. Suzana Hadjur

• Curated by eLife

  eLife assessment

  This potentially valuable study reports new and unexpected roles of STAG3 in regulating exit from pluripotency in mouse embryonic stem cells (mESCs). However, the evidence for the proposed role of STAG3 in the post-transcriptional regulation of gene expression is viewed as yet incomplete. The work will be of interest to colleagues studying stem cells, early steps in differentiation, and gene regulation.

Reviewed by eLife

PP2A-B56 regulates Mid1 protein levels for proper cytokinesis in fission yeast

1. Madeline L. Chrupcala
2. James B. Moseley

Reviewed by Review Commons

Nuclear basket proteins regulate the distribution and mobility of nuclear pore complexes in budding yeast

1. Janka Zsok
2. Francois Simon
3. Göksu Bayrak
4. Luljeta Isaki
5. Nina Kerff
6. Yoana Kicheva
7. Amy Wolstenholme
8. Lucien E Weiss
9. Elisa Dultz

Reviewed by Review Commons