Cell Biology

FAM210A mediates an inter-organelle crosstalk essential for protein synthesis and muscle growth in mouse

1. Jingjuan Chen
2. Feng Yue
3. Kun Ho Kim
4. Peipei Zhu
5. Jiamin Qiu
6. W. Andy Tao
7. Shihuan Kuang

Reviewed by Review Commons

Pathogenic aggregates alter actin organization and cellular viscosity resulting in stalled clathrin mediated endocytosis

1. Surya Bansi Singh
2. Shatruhan Singh Rajput
3. Aditya Sharma
4. Vaishnavi Ananthanarayanan
5. Amitabha Nandi
6. Shivprasad Patil
7. Amitabha Majumdar
8. Deepa Subramanyam

Reviewed by Review Commons, Arcadia Science

Chemotherapy activates inflammasomes to cause inflammation-associated bone loss

1. Chun Wang
2. Khushpreet Kaur
3. Canxin Xu
4. Yousef Abu-Amer
5. Gabriel Mbalaviele

• Curated by eLife

  eLife assessment

  This useful study, which systematically addresses off-target effects of a commonly used chemotherapy drug on bone and bone marrow cells and which therefore is of potential interest to a broad readership, presents evidence that reducing systemic inflammation induced by doxorubicin limits bone loss to some extent. The demonstration of the effect of systemic inflammation on bone loss is convincing. Building on prior work, this study sets the scene for additional genetic and pharmacologic experiments as well as future analyses of the bone phenotypes, which should speak to the mechanisms involved in doxorubicin-induced bone loss – which are not addressed in the current study – and which may substantiate the clinical relevance of targeting inflammation in order to limit the negative impact of chemotherapies on bone quality.

Reviewed by eLife

A “torn bag mechanism” of small extracellular vesicle release via limiting membrane rupture of en bloc released amphisomes (amphiectosomes)

1. Tamás Visnovitz
2. Dorina Lenzinger
3. Anna Koncz
4. Péter M Vizi
5. Tünde Bárkai
6. Krisztina V Vukman
7. Alicia Galinsoga
8. Krisztina Németh
9. Kelsey Fletcher
10. Zsolt I Komlósi
11. Péter Lőrincz
12. Gábor Valcz
13. Edit I Buzás

• Curated by eLife

  eLife assessment

  The authors present evidence that small extracellular vesicles can be secreted from cells inside larger vesicles that they call amphiectosomes, which then tear to release their small vesicle contents. There are questions and concerns relating to the quality of the data and the in vivo significance of the observations. The findings are potentially important but the data are incomplete and the claims are only partially supported.

Reviewed by eLife

DHCR24-mediated sterol homeostasis during spermatogenesis is required for sperm mitochondrial sheath formation and impacts male fertility over time

1. Sona Relovska
2. Huafeng Wang
3. Xinbo Zhang
4. Pablo Fernández-Tussy
5. Kyung Jo Jeong
6. Jungmin Choi
7. Yajaira Suárez
8. Jeffrey G. McDonald
9. Carlos Fernández-Hernando
10. Jean-Ju Chung

• Curated by eLife

  eLife assessment

  This useful study reports data supporting the importance of sterol homeostasis in sperm development and consequently male reproduction. While most of the data are supportive of the conclusion, some remain incomplete and need more experimental verification. This work would be of interest to basic researchers and clinicians working on sterol homeostasis and male fertility.

Reviewed by eLife

Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies

1. Mathieu C Husser
2. Nhat P Pham
3. Chris Law
4. Flavia RB Araujo
5. Vincent JJ Martin
6. Alisa Piekny

• Curated by eLife

  eLife assessment

  In this study, the authors develop a strategy for fluorophore-tagging endogenous proteins in human induced pluripotent stem cells (iPSCs) using a split mNeonGreen approach, and they conclude that the system will be appropriate for performing live imaging studies of highly dynamic cellular processes such as cytokinesis in iPSCs. Experimentally, the methods are solid, and the data presented support the authors' conclusions. Overall, these methodologies should be useful to a wide audience of cell biologists who want to study protein localization and dynamics at endogenous levels in iPSCs.

Reviewed by eLife

Cytoplasmic protein-free mRNA induces stress granules by two G3BP1/2-dependent mechanisms

1. Sean J. Ihn
2. Laura Farlam-Williams
3. Alexander F. Palazzo
4. Hyun O. Lee

Reviewed by PREreview

Wound-Induced Syncytia Outpace Mononucleate Neighbors during Drosophila Wound Repair

1. James S. White
2. Jasmine J. Su
3. Elizabeth M. Ruark
4. Junmin Hua
5. M. Shane Hutson
6. Andrea Page-McCaw

• Curated by eLife

  eLife assessment

  This work addresses an important biological question: what is the cellular basis of wound healing? Using the Drosophila pupal notum as a model, the paper provides an elegant, thorough, descriptive characterisation of syncytia-driven wound closure using state-of-the-art confocal live imaging of the pupal notum. The authors meticulously characterize the cell-cell fusion events during wound healing, but without any mechanisms to inhibit cell fusion, it is incomplete, since it remains unclear whether cell fusion is required or not for speeding wound healing and/ or increasing the level of actin resources at the leading edge.

Reviewed by eLife

Structural Basis for Mis18 Complex Assembly: Implications for Centromere Maintenance

1. Reshma Thamkachy
2. Bethan Medina-Pritchard
3. Sang Ho Park
4. Carla G. Chiodi
5. Juan Zou
6. Maria de la Torre-Barranco
7. Kazuma Shimanaka
8. Maria Alba Abad
9. Cristina Gallego Páramo
10. Regina Feederle
11. Emilija Ruksenaite
12. Patrick Heun
13. Owen R. Davies
14. Juri Rappsilber
15. Dina Schneidman-Duhovny
16. Uhn-Soo Cho
17. A. Arockia Jeyaprakash

Reviewed by Review Commons

Axonal distribution of mitochondria maintains neuronal autophagy during aging via eIF2β

1. Kanako Shinno
2. Yuri Miura
3. Koichi M. Iijima
4. Emiko Suzuki
5. Kanae Ando

• Curated by eLife

  eLife assessment:

  In flies defective for axonal transport of mitochondria, the authors report the upregulation of one subunit, the beta subunit, of the heterotrimeric eIF2 complex via mass spectroscopy proteome analysis. Neuronal overexpression of eIF2β phenocopied aspects of neuronal dysfunction observed when axonal transport of mitochondria was compromised. Conversely, lowering eIF2β expression suppressed aspects of neuronal dysfunction. While these are intriguing observations that are potentially useful, several technical weaknesses limit the interpretation and mean the evidence supporting the current claims is incomplete.

Reviewed by eLife