Cell Biology

Intestinal stem cell renewal controlled by capillary morphogenesis gene 2 following injury

1. Lucie Bracq
2. Audrey Chuat
3. Béatrice Kunz
4. Olivier Burri
5. Romain Guiet
6. F. Gisou van der Goot

Reviewed by Review Commons

The ESCRT protein CHMP5 restricts bone formation by controlling endolysosome-mitochondrion-mediated cell senescence

1. Fan Zhang
2. Yuan Wang
3. Luyang Zhang
4. Chunjie Wang
5. Deping Chen
6. Haibo Liu
7. Ren Xu
8. Cole M Haynes
9. Jae-Hyuck Shim
10. Xianpeng Ge

• Curated by eLife

  eLife Assessment

  This important work advances our understanding of CHMP5's role in regulating osteogenesis through its impact on cellular senescence. The evidence supporting the conclusion is convincing and the revised manuscript is largely improved. This paper holds potential interest for skeletal biologists who study the pathogenesis of age-associated skeletal disorders.

Reviewed by eLife

Clearance of protein aggregates during cell division

1. Shoukang Du
2. Yuhan Wang
3. Bowen Chen
4. Shuangshuang Xie
5. Kuan Yoow Chan
6. David C Hay
7. Ting Gang Chew

• Curated by eLife

  eLife Assessment

  How misfolded proteins are segregated and cleared is a significant question in cell biology, since clearance of these aggregates can protect against pathologies that may otherwise arise. The authors discover a cell cycle stage-dependent clearing mechanism that involves the ER chaperone BiP, the proteosome, and CDK inactivation, but is curiously independent of the anaphase promoting complex (APC). These are valuable and interesting new observations, and the evidence supporting these claims is solid.

Reviewed by eLife

Profiling the physiological impact of aberrant folded-state protein filamentation in cells

1. Tal Levin
2. Hector Garcia-Seisdedos
3. Arseniy Lobov
4. Matthias Wojtynek
5. Alexander Alexandrov
6. Ghil Jona
7. Dikla Levi
8. Ohad Medalia
9. D. Emmanuel Levy

Reviewed by Review Commons

N-cadherin mechanosensing in ovarian follicles controls oocyte maturation and ovulation

1. Alaknanda Emery
2. Orest W Blaschuk
3. Doan T Dinh
4. Tim McPhee
5. Rouven Becker
6. Andrew D Abell
7. Krzysztof M Mrozik
8. Andrew CW Zannettino
9. Rebecca L Robker
10. Darryl L Russell

• Curated by eLife

  eLife assessment

  The manuscript describes important findings regarding the significance of CHD2 in ovarian folliculogenesis. Overall, the results lead to convincing conclusions, with minimal concerns raised by the reviewers. Both the results and conclusions are well discussed. This work will be of interest to ovarian biologists and physicians working on female fertility.

Reviewed by eLife

Kinesin-2 autoinhibition requires elbow phosphorylation

1. Guanghan Chen
2. Zhengyang Guo
3. Zhiwen Zhu
4. Shanshan Xie
5. Tianhua Zhou
6. Guangshuo Ou

• Curated by eLife

  eLife Assessment

  In their important manuscript, Chen et al. investigate the phospho-regulation of the C. elegans kinesin-2 motor protein OSM-3, revealing that the kinase, NEKL-3, phosphorylates a serine/threonine patch at the hinge region of the motor to mediate autoinhibition until it reaches the ciliary middle segment. The findings are supported by robust genetic data, in vivo imaging, and motility assays with wild-type and mutant motors. Overall, the study provides a compelling contribution to understanding the regulation of OSM-3 kinesin activity both on the molecular and cellular levels.

Reviewed by eLife

A novel approach to tagging tubulin reveals MT assembly dynamics of the axoneme in Trypanosoma brucei

1. Daniel Abbühl
2. Martina Pružincová
3. Luděk Štěpánek
4. Eleonore Bouscasse
5. Rita Azevedo
6. Mariette Matondo
7. Vladimir Varga
8. Serge Bonnefoy
9. Philippe Bastin

Reviewed by Review Commons

Image Correlation Spectroscopy is a Robust Tool to Quantify Cellular DNA Damage Response

1. Angelica A Gopal
2. Bianca Fernandez
3. Paul W Wiseman
4. J Matthew Dubach

• Curated by eLife

  eLife Assessment

  This valuable paper shows image correlation spectroscopy (ICS) as a new tool to analyze the clustering of proteins involved in DNA damage response (DDR). The convincing evidence presented demonstrates that ICS is more sensitive than traditional foci counting. This new method provides an alternative tool to quantify immunostained foci for researchers in the fields of DDR and cell biology.

Reviewed by eLife

p66Shc Mediates SUMO2-induced Endothelial Dysfunction

1. Jitendra Kumar
2. Shravan K Uppulapu
3. Sujata Kumari
4. Kanika Sharma
5. William Paradee
6. Ravi Prakash Yadav
7. Vikas Kumar
8. Santosh Kumar

• Curated by eLife

  eLife Assessment

  This study offers valuable insights into the role of post-translational modifiers, specifically SUMO2ylation at K81 in p66Shc, and its impact on endothelial function through reactive oxygen species. A series of compelling experiments demonstrated that lysine 81 of p66Shc is the site of SUMO2 conjugation, which is crucial for mitochondrial localization and essential for S36 phosphorylation, leading to specific pathological effects. The combination of cell overexpression and animal studies provides solid data supporting this mechanistic link.

Reviewed by eLife

Mutations that prevent phosphorylation of the BMP4 prodomain impair proteolytic maturation of homodimers leading to lethality in mice

1. Hyung-Seok Kim
2. Mary L Sanchez
3. Joshua Silva
4. Heidi L Schubert
5. Rebecca Dennis
6. Christopher P Hill
7. Jan L Christian

• Curated by eLife

  eLife Assessment

  This fundamental work presents two clinically relevant BMP4 mutations that contribute to vertebrate development. The compelling evidence, both from wet lab and AI generated predictions, supports that the site-specific cleavage at the BMP4 pro-domain precisely regulates its function and provides mechanistic insight how homodimers and heterodimers behave differently. The work will be of broad interest to researchers working on growth factor signaling mechanisms and vertebrate development.

Reviewed by eLife