Bioengineering

Development of a highly active engineered PETase enzyme for polyester degradation

1. Shapla Bhattacharya
2. Rossella Castagna
3. Hajar Estiri
4. Toms Upmanis
5. Andrea Ricci
6. Alfonso Gautieri
7. Emilio Parisini

Reviewed by Arcadia Science

Programming Aliphatic Polyester Degradation by Engineered Bacterial Spores

1. Ziyu Cui
2. Masamu Kawada
3. Yue Hui
4. Seunghyun Sim

Reviewed by Arcadia Science

Rapid protein evolution by few-shot learning with a protein language model

1. Kaiyi Jiang
2. Zhaoqing Yan
3. Matteo Di Bernardo
4. Samantha R. Sgrizzi
5. Lukas Villiger
6. Alisan Kayabolen
7. Byungji Kim
8. Josephine K. Carscadden
9. Masahiro Hiraizumi
10. Hiroshi Nishimasu
11. Jonathan S. Gootenberg
12. Omar O. Abudayyeh

Reviewed by Arcadia Science

Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cells

1. Srishti U. Sahu
2. Madalena Castro
3. Joseph J. Muldoon
4. Kunica Asija
5. Stacia K. Wyman
6. Netravathi Krishnappa
7. Lorena de Oñate
8. Justin Eyquem
9. David N. Nguyen
10. Ross C. Wilson

Reviewed by Arcadia Science

ESPRESSO: Spatiotemporal omics based on organelle phenotyping

1. Lorenzo Scipioni
2. Giulia Tedeschi
3. Mariana Navarro
4. Yunlong Jia
5. Scott Atwood
6. Jennifer A. Prescher
7. Michelle Digman

Reviewed by Arcadia Science

CYpHER: catalytic extracellular targeted protein degradation with high potency and durable effect

1. Zachary R. Crook
2. Gregory P. Sevilla
3. Pamela Young
4. Emily J. Girard
5. Tinh-Doan Phi
6. Monique L. Howard
7. Jason Price
8. James M. Olson
9. Natalie W. Nairn

Reviewed by PREreview

A three filament mechanistic model of musculotendon force and impedance

1. Matthew Millard
2. David W Franklin
3. Walter Herzog

• Curated by eLife

  eLife assessment

  This is a valuable study that develops a new model of the way muscle responds to perturbations, synthesizing models of how it responds to small and large perturbations, both of which are used to predict how muscles function for stability but also how they can be injured, and which tend to be predicted poorly by classic Hill-type models. The evidence presented to support the model is solid, since it outperforms Hill-type models in a variety of conditions. Although the combination of phenomenological and mechanistic aspects of the model may sometimes make it challenging to interpret the output, the work will be of interest to those developing realistic models of the stability and control of movement in humans or other animals.

Reviewed by eLife

Overcoming the nutritional immunity by engineering iron-scavenging bacteria for cancer therapy

1. Sin-Wei Huang
2. See-Khai Lim
3. Yao-An Yu
4. Yi-Chung Pan
5. Wan-Ju Lien
6. Chung-Yuan Mou
7. Che-Ming Jack Hu
8. Kurt Yun Mou

• Curated by eLife

  eLife assessment

  This valuable study combines proteomics and a mouse model to reveal the importance of iron uptake in bacterial therapy for cancer. The evidence presented is convincing. Notably, the authors showed upregulation of iron uptake of bacteria significantly inhibits tumor growth in vivo. This paper will be of interest to a broad audience including researchers in cancer biology, cell biology, and microbiology.

Reviewed by eLife

Near-perfect precise on-target editing of human hematopoietic stem and progenitor cells

1. Fanny-Mei Cloarec-Ung
2. Jamie Beaulieu
3. Arunan Suthananthan
4. Bernhard Lehnertz
5. Guy Sauvageau
6. Hilary M Sheppard
7. David JHF Knapp

• Curated by eLife

  eLife assessment

  This study presents an important methodology to increase the efficiency and precision of gene editing in human hematopoietic stem and progenitor cells. The evidence supporting the claims is convincing in that primitive LTC-ICs were minimally affected as a result of the editing procedure and the lack of edits at predicted off-target sites. The work will be of interest to biologists studying hematopoietic stem and progenitor cells and genome editing for potential clinical applications.

Reviewed by eLife

A versatile high-throughput assay based on 3D ring-shaped cardiac tissues generated from human induced pluripotent stem cell-derived cardiomyocytes

1. Magali Seguret
2. Patricia Davidson
3. Stijn Robben
4. Charlène Jouve
5. Celine Pereira
6. Quitterie Lelong
7. Lucille Deshayes
8. Cyril Cerveau
9. Maël Le Berre
10. Rita S Rodrigues Ribeiro
11. Jean-Sébastien Hulot

• Curated by eLife

  eLife assessment

  This paper reports a valuable platform for cardiac tissue cultivation. The throughput, consistency of the tissue, and the potential integration of high-throughput automation are an advantage over other approaches. The tissues and the platform are validated using appropriate methodology to provide convincing evidence of the tissue cultivation capability.

Reviewed by eLife