Bioengineering

A three filament mechanistic model of musculotendon force and impedance

1. Matthew Millard
2. David W Franklin
3. Walter Herzog

• Curated by eLife

  eLife assessment

  This is a valuable study that develops a new model of the way muscle responds to perturbations, synthesizing models of how it responds to small and large perturbations, both of which are used to predict how muscles function for stability but also how they can be injured, and which tend to be predicted poorly by classic Hill-type models. The evidence presented to support the model is solid, since it outperforms Hill-type models in a variety of conditions. Although the combination of phenomenological and mechanistic aspects of the model may sometimes make it challenging to interpret the output, the work will be of interest to those developing realistic models of the stability and control of movement in humans or other animals.

Reviewed by eLife

Overcoming the nutritional immunity by engineering iron-scavenging bacteria for cancer therapy

1. Sin-Wei Huang
2. See-Khai Lim
3. Yao-An Yu
4. Yi-Chung Pan
5. Wan-Ju Lien
6. Chung-Yuan Mou
7. Che-Ming Jack Hu
8. Kurt Yun Mou

• Curated by eLife

  eLife assessment

  This valuable study combines proteomics and a mouse model to reveal the importance of iron uptake in bacterial therapy for cancer. The evidence presented is convincing. Notably, the authors showed upregulation of iron uptake of bacteria significantly inhibits tumor growth in vivo. This paper will be of interest to a broad audience including researchers in cancer biology, cell biology, and microbiology.

Reviewed by eLife

Near-perfect precise on-target editing of human hematopoietic stem and progenitor cells

1. Fanny-Mei Cloarec-Ung
2. Jamie Beaulieu
3. Arunan Suthananthan
4. Bernhard Lehnertz
5. Guy Sauvageau
6. Hilary M Sheppard
7. David JHF Knapp

• Curated by eLife

  eLife assessment

  This study presents an important methodology to increase the efficiency and precision of gene editing in human hematopoietic stem and progenitor cells. The evidence supporting the claims is convincing in that primitive LTC-ICs were minimally affected as a result of the editing procedure and the lack of edits at predicted off-target sites. The work will be of interest to biologists studying hematopoietic stem and progenitor cells and genome editing for potential clinical applications.

Reviewed by eLife

A versatile high-throughput assay based on 3D ring-shaped cardiac tissues generated from human induced pluripotent stem cell-derived cardiomyocytes

1. Magali Seguret
2. Patricia Davidson
3. Stijn Robben
4. Charlène Jouve
5. Celine Pereira
6. Quitterie Lelong
7. Lucille Deshayes
8. Cyril Cerveau
9. Maël Le Berre
10. Rita S Rodrigues Ribeiro
11. Jean-Sébastien Hulot

• Curated by eLife

  eLife assessment

  This paper reports a valuable platform for cardiac tissue cultivation. The throughput, consistency of the tissue, and the potential integration of high-throughput automation are an advantage over other approaches. The tissues and the platform are validated using appropriate methodology to provide convincing evidence of the tissue cultivation capability.

Reviewed by eLife

Automated cell annotation in multi-cell images using an improved CRF_ID algorithm

1. Hyun Jee Lee
2. Jingting Liang
3. Shivesh Chaudhary
4. Sihoon Moon
5. Zikai Yu
6. Taihong Wu
7. He Liu
8. Myung-Kyu Choi
9. Yun Zhang
10. Hang Lu

• Curated by eLife

  eLife Assessment

  This Research Advance describes a valuable image analysis method to identify individual neurons within a ‎population of fluorescently labeled cells in the nematode C. elegans. The findings are solid and the method succeeds to identify cells with high precision. The method will be be of interest to the C. elegans research community.

Reviewed by eLife

Endo-Lysosome-Targeted Nanoparticle Delivery of Antiviral Therapy for Coronavirus Infections

1. Anton Petcherski
2. Brett M Tingley
3. Andrew Martin
4. Sarah Adams
5. Alexandra J Brownstein
6. Ross A Steinberg
7. Byourak Shabane
8. Jennifer Ngo
9. Corey Osto
10. Gustavo Garcia
11. Michaela Veliova
12. Vaithilingaraja Arumugaswami
13. Aaron H Colby
14. Orian S Shirihai
15. Mark W Grinstaff

Reviewed by Review Commons

The vascularised chamber device significantly enhances the survival of transplanted liver organoids

1. Denis D. Shi
2. Evelyn Makris
3. Yi-Wen Gerrand
4. Pu-Han Lo
5. George C. Yeoh
6. Wayne A. Morrison
7. Geraldine M. Mitchell
8. Kiryu K. Yap

• Curated by eLife

  eLife assessment

  This solid manuscript describes a preclinical model to assess different methods of infusion of organoids for clinical applications. This is an important and timely study with practical implications beyond a single subfield. The methods described, including the analysis, broadly support the claims although there are some areas for improvement.

Reviewed by eLife

Technical upgrade of an open-source liquid handler to support bacterial colony screening

1. Irene del Olmo
2. Pablo Yubero
3. Álvaro Gómez-Luengo
4. Juan Nogales
5. David R. Espeso

Reviewed by Arcadia Science

Targeting sex determination to suppress mosquito populations

1. Ming Li
2. Nikolay P Kandul
3. Ruichen Sun
4. Ting Yang
5. Elena D Benetta
6. Daniel J Brogan
7. Igor Antoshechkin
8. Héctor M Sánchez C
9. Yinpeng Zhan
10. Nicolas A DeBeaubien
11. YuMin M Loh
12. Matthew P Su
13. Craig Montell
14. John M Marshall
15. Omar S Akbari

• Curated by eLife

  eLife assessment

  This valuable paper builds on a method, previously conceptualized and validated, of genetic control for insect populations. The method, called pgSIT, uses integrated CRISPR-Cas9 based constructs to generate, in certain combinations of genotypes, mutations that cause both male sterility and female inviability. Release of such genotypes in sufficiently large numbers can lead to an inundation of a local insect population with sterile males and this can lead to localised population suppression, which represents an effective method of control for problematic insect populations. The data are convincing and will be of interest to anyone working on vector control strategies.

Reviewed by eLife

Synergistic induction of blood–brain barrier properties

1. Gergő Porkoláb
2. Mária Mészáros
3. Anikó Szecskó
4. Judit P. Vigh
5. Fruzsina R. Walter
6. Ricardo Figueiredo
7. Ildikó Kálomista
8. Zsófia Hoyk
9. Gaszton Vizsnyiczai
10. Ilona Gróf
11. Jeng-Shiung Jan
12. Fabien Gosselet
13. Melinda K. Pirity
14. Monika Vastag
15. Natalie Hudson
16. Matthew Campbell
17. Szilvia Veszelka
18. Mária A. Deli

Reviewed by PREreview