Bioengineering

Identification of pharmacological inducers of a reversible hypometabolic state for whole organ preservation

1. Megan M Sperry
2. Berenice Charrez
3. Haleh Fotowat
4. Erica Gardner
5. Kanoelani Pilobello
6. Zohreh Izadifar
7. Tiffany Lin
8. Abigail Kuelker
9. Sahith Kaki
10. Michael Lewandowski
11. Shanda Lightbown
12. Ramses Martinez
13. Susan Marquez
14. Joel Moore
15. Maria Plaza-Oliver
16. Adama M Sesay
17. Kostyantyn Shcherbina
18. Katherine Sheehan
19. Takako Takeda
20. Daniela Del Campo
21. Kristina Andrijauskaite
22. Exal Cisneros
23. Riley Lopez
24. Isabella Cano
25. Zachary Maxwell
26. Israel Jessop
27. Rafa Veraza
28. Leon Bunegin
29. Thomas J Percival
30. Jaclyn Yracheta
31. Jorge J Pena
32. Diandra M Wood
33. Zachary T Homas
34. Cody J Hinshaw
35. Jennifer Cox-Hinshaw
36. Olivia G Parry
37. Justin J Sleeter
38. Erik K Weitzel
39. Michael Levin
40. Michael Super
41. Richard Novak
42. Donald E Ingber

• Curated by eLife

  eLife assessment

  Pharmacological induction of physiological slowing combined with organ perfusion systems could provide a novel therapeutic strategy for tissue and organ preservation. Using a Xenopus model, the authors provide important findings on a use of drug to slow down metabolism for the purpose of organ preservation. The authors provide compelling evidence that SNC80 can rapidly and reversibly slow biochemical and metabolic activities while preserving cell and tissue viability. This approach may be beneficial for transplantation, trauma management, and improving organ survival in remote and low-resource settings

Reviewed by eLife

Development of a highly active engineered PETase enzyme for polyester degradation

1. Shapla Bhattacharya
2. Rossella Castagna
3. Hajar Estiri
4. Toms Upmanis
5. Andrea Ricci
6. Alfonso Gautieri
7. Emilio Parisini

Reviewed by Arcadia Science

Programming Aliphatic Polyester Degradation by Engineered Bacterial Spores

1. Ziyu Cui
2. Masamu Kawada
3. Yue Hui
4. Seunghyun Sim

Reviewed by Arcadia Science

Rapid protein evolution by few-shot learning with a protein language model

1. Kaiyi Jiang
2. Zhaoqing Yan
3. Matteo Di Bernardo
4. Samantha R. Sgrizzi
5. Lukas Villiger
6. Alisan Kayabolen
7. Byungji Kim
8. Josephine K. Carscadden
9. Masahiro Hiraizumi
10. Hiroshi Nishimasu
11. Jonathan S. Gootenberg
12. Omar O. Abudayyeh

Reviewed by Arcadia Science

Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cells

1. Srishti U. Sahu
2. Madalena Castro
3. Joseph J. Muldoon
4. Kunica Asija
5. Stacia K. Wyman
6. Netravathi Krishnappa
7. Lorena de Oñate
8. Justin Eyquem
9. David N. Nguyen
10. Ross C. Wilson

Reviewed by Arcadia Science

ESPRESSO: Spatiotemporal omics based on organelle phenotyping

1. Lorenzo Scipioni
2. Giulia Tedeschi
3. Mariana Navarro
4. Yunlong Jia
5. Scott Atwood
6. Jennifer A. Prescher
7. Michelle Digman

Reviewed by Arcadia Science

CYpHER: catalytic extracellular targeted protein degradation with high potency and durable effect

1. Zachary R. Crook
2. Gregory P. Sevilla
3. Pamela Young
4. Emily J. Girard
5. Tinh-Doan Phi
6. Monique L. Howard
7. Jason Price
8. James M. Olson
9. Natalie W. Nairn

Reviewed by PREreview

A three filament mechanistic model of musculotendon force and impedance

1. Matthew Millard
2. David W Franklin
3. Walter Herzog

• Curated by eLife

  eLife assessment

  This is a valuable study that develops a new model of the way muscle responds to perturbations, synthesizing models of how it responds to small and large perturbations, both of which are used to predict how muscles function for stability but also how they can be injured, and which tend to be predicted poorly by classic Hill-type models. The evidence presented to support the model is solid, since it outperforms Hill-type models in a variety of conditions. Although the combination of phenomenological and mechanistic aspects of the model may sometimes make it challenging to interpret the output, the work will be of interest to those developing realistic models of the stability and control of movement in humans or other animals.

Reviewed by eLife

Overcoming the nutritional immunity by engineering iron-scavenging bacteria for cancer therapy

1. Sin-Wei Huang
2. See-Khai Lim
3. Yao-An Yu
4. Yi-Chung Pan
5. Wan-Ju Lien
6. Chung-Yuan Mou
7. Che-Ming Jack Hu
8. Kurt Yun Mou

• Curated by eLife

  eLife assessment

  This valuable study combines proteomics and a mouse model to reveal the importance of iron uptake in bacterial therapy for cancer. The evidence presented is convincing. Notably, the authors showed upregulation of iron uptake of bacteria significantly inhibits tumor growth in vivo. This paper will be of interest to a broad audience including researchers in cancer biology, cell biology, and microbiology.

Reviewed by eLife

Near-perfect precise on-target editing of human hematopoietic stem and progenitor cells

1. Fanny-Mei Cloarec-Ung
2. Jamie Beaulieu
3. Arunan Suthananthan
4. Bernhard Lehnertz
5. Guy Sauvageau
6. Hilary M Sheppard
7. David JHF Knapp

• Curated by eLife

  eLife assessment

  This study presents an important methodology to increase the efficiency and precision of gene editing in human hematopoietic stem and progenitor cells. The evidence supporting the claims is convincing in that primitive LTC-ICs were minimally affected as a result of the editing procedure and the lack of edits at predicted off-target sites. The work will be of interest to biologists studying hematopoietic stem and progenitor cells and genome editing for potential clinical applications.

Reviewed by eLife