Bioengineering

Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation

1. Mami Matsuo-Takasaki
2. Sho Kambayashi
3. Yasuko Hemmi
4. Tamami Wakabayashi
5. Tomoya Shimizu
6. Yuri An
7. Hidenori Ito
8. Kazuhiro Takeuchi
9. Masato Ibuki
10. Terasu Kawashima
11. Rio Masayasu
12. Manami Suzuki
13. Yoshikazu Kawai
14. Masafumi Umekage
15. Tomoaki M Kato
16. Michiya Noguchi
17. Koji Nakade
18. Yukio Nakamura
19. Tomoyuki Nakaishi
20. Naoki Nishishita
21. Masayoshi Tsukahara
22. Yohei Hayashi

• Curated by eLife

  eLife Assessment

  This comprehensive and compelling study presents a robust, cost-effective method for expanding pluripotent stem cells. The authors have identified a media condition that maintains iPSCs in suspension cultures by inhibiting the PKCβ and Wnt signaling pathways. The manuscript is important for the pluripotent stem cell field as it seeks robust and economical approaches to expand iPSCs at scale for high throughput screens and preclinical studies. While the authors have tested their media and protocol on a few lines, given the variability of iPSCs, further testing across more cell lines and in different laboratory settings will be crucial to evaluate its reproducibility.

Reviewed by eLife

High-throughput expansion microscopy enables scalable super-resolution imaging

1. John H Day
2. Catherine M Della Santina
3. Pema Maretich
4. Alexander L Auld
5. Kirsten K Schnieder
6. Tay Shin
7. Edward S Boyden
8. Laurie A Boyer

• Curated by eLife

  eLife Assessment

  This important study develops a high throughput version of expansion microscopy that can be performed in 96-well plates. The engineered technology is convincing and compatible with standard microplates and automated microscopes and thus will be of broad interest. The application to hiPCS-derived cardiomyocytes treated with doxorubicin provides a solid proof-of-concept demonstrating the potential for high-throughput analysis.

Reviewed by eLife

Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections

1. Mighten C Yip
2. Mercedes M Gonzalez
3. Colby F Lewallen
4. Corey R Landry
5. Ilya Kolb
6. Bo Yang
7. William M Stoy
8. Ming-fai Fong
9. Matthew JM Rowan
10. Edward S Boyden
11. Craig R Forest

• Curated by eLife

  eLife Assessment

  This technical study presents a novel sampling strategy for detecting synaptic coupling between neurons from dual pipette patch-clamp recordings in acute slices of mammalian brain tissue in vitro. The authors present solid evidence that this strategy, which incorporates automated patch clamp electrode positioning and cleaning for reuse with strategic neuron targeting, has the potential to substantially improve the efficiency of neuronal sampling with paired recordings. This technique and the extensions discussed will be useful for neuroscientists wanting to apply or already conducting automated multi-pipette patch clamp recording electrophysiology experiments in vitro for neuron connectivity analyses.

Reviewed by eLife

Development of a new genotype–phenotype linked antibody screening system

1. Takashi Watanabe
2. Hikaru Hata
3. Yoshiki Mochizuki
4. Fumie Yokoyama
5. Tomoko Hasegawa
6. Naveen Kumar
7. Tomohiro Kurosaki
8. Osamu Ohara
9. Hidehiro Fukuyama

• Curated by eLife

  eLife Assessment

  The useful studies described here are broadly applicable to all antibody discovery subfields, even though they are not a significant improvement over published methods. The findings are incomplete with respect to the methodology, since details that are crucial in order to repeat the experiments are lacking (such as a timestamp). They also do not take into account multiple recent papers that have tested similar strategies. These studies will be of interest to a specialized audience working on generating antibodies to infectious agents.

Reviewed by eLife

Engineered Nanotopographies Induce Transient Openings in the Nuclear Membrane

1. Einollah Sarikhani
2. Vrund Patel
3. Zhi Li
4. Dhivya Pushpa Meganathan
5. Keivan Rahmani
6. Leah Sadr
7. Ryan Hosseini
8. Diether Visda
9. Shivani Shukla
10. Hamed Naghsh‐Nilchi
11. Adarsh Balaji
12. Gillian McMahon
13. Shaoming Chen
14. Johannes Schöneberg
15. Colleen A. McHugh
16. Lingyan Shi
17. Zeinab Jahed

Reviewed by preLights

Two mechanisms of photoenergy regulation revealed by kinetic behaviors of chlorophyll fluorescence during light adaptation

1. Junqing Chen
2. Lijiang Fu
3. Ya Guo
4. Jinglu Tan

Reviewed by Arcadia Science

A 3D-printed handheld device for quick citrus tissue lysis and nucleic acid extraction

1. Chia-Wei Liu
2. Brent Kalish
3. Sohrab Bodaghi
4. Georgios Vidalakis
5. Hideaki Tsutsui

Reviewed by Arcadia Science

Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

1. Qingqing Feng
2. Keman Cheng
3. Lizhuo Zhang
4. Xiaoyu Gao
5. Jie Liang
6. Guangna Liu
7. Nana Ma
8. Chen Xu
9. Ming Tang
10. Liting Chen
11. Xinwei Wang
12. Xuehui Ma
13. Jiajia Zou
14. Quanwei Shi
15. Pei Du
16. Qihui Wang
17. Guangjun Nie
18. Xiao Zhao

Reviewed by Rapid Reviews Infectious Diseases

Il-6 signaling exacerbates hallmarks of chronic tendon disease by stimulating reparative fibroblasts

1. Tino Stauber
2. Greta Moschini
3. Amro A Hussien
4. Patrick Klaus Jaeger
5. Katrien De Bock
6. Jess G Snedeker

• Curated by eLife

  eLife assessment

  This important study defines signaling mechanisms in tendinopathy development, which is significant as there is a clear need to identify therapeutic targets to prevent or reverse tendon pathology. The evidence supporting the conclusions are compelling combining an existing human tendinopathy transcriptomics dataset with ex-vivo assembloid model, and an in vivo injury model using genetic reporter mice. This work will be of interest to developmental and stem cell biologists.

Reviewed by eLife

Identification of pharmacological inducers of a reversible hypometabolic state for whole organ preservation

1. Megan M Sperry
2. Berenice Charrez
3. Haleh Fotowat
4. Erica Gardner
5. Kanoelani Pilobello
6. Zohreh Izadifar
7. Tiffany Lin
8. Abigail Kuelker
9. Sahith Kaki
10. Michael Lewandowski
11. Shanda Lightbown
12. Ramses Martinez
13. Susan Marquez
14. Joel Moore
15. Maria Plaza-Oliver
16. Adama M Sesay
17. Kostyantyn Shcherbina
18. Katherine Sheehan
19. Takako Takeda
20. Daniela Del Campo
21. Kristina Andrijauskaite
22. Exal Cisneros
23. Riley Lopez
24. Isabella Cano
25. Zachary Maxwell
26. Israel Jessop
27. Rafa Veraza
28. Leon Bunegin
29. Thomas J Percival
30. Jaclyn Yracheta
31. Jorge J Pena
32. Diandra M Wood
33. Zachary T Homas
34. Cody J Hinshaw
35. Jennifer Cox-Hinshaw
36. Olivia G Parry
37. Justin J Sleeter
38. Erik K Weitzel
39. Michael Levin
40. Michael Super
41. Richard Novak
42. Donald E Ingber

• Curated by eLife

  eLife assessment

  Pharmacological induction of physiological slowing combined with organ perfusion systems could provide a novel therapeutic strategy for tissue and organ preservation. Using a Xenopus model, the authors provide important findings on a use of drug to slow down metabolism for the purpose of organ preservation. The authors provide compelling evidence that SNC80 can rapidly and reversibly slow biochemical and metabolic activities while preserving cell and tissue viability. This approach may be beneficial for transplantation, trauma management, and improving organ survival in remote and low-resource settings

Reviewed by eLife