eLife

Latest preprint reviews

1. Competitive coordination of the dual roles of the Hedgehog co-receptor in homophilic adhesion and signal reception

   This article has 10 authors:

   1. Shu Yang
   2. Ya Zhang
   3. Chuxuan Yang
   4. Xuefeng Wu
   5. Sarah Maria El Oud
   6. Rongfang Chen
   7. Xudong Cai
   8. Xufeng S Wu
   9. Ganhui Lan
   10. Xiaoyan Zheng

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is a well-conceived and well-presented study that sheds light on two critical questions related to Hedgehog signaling, namely, the dual function of the Hedgehog co-receptor Ihog in Hh signal transduction and homophilic adhesion, and the regulation of cytoneme structure.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

2. A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis

   This article has 15 authors:

   1. Jia Li
   2. Xuan Qin
   3. Jie Shi
   4. Xiaoshuang Wang
   5. Tong Li
   6. Mengyao Xu
   7. Xiaosu Chen
   8. Yujia Zhao
   9. Jiahao Han
   10. Yongjun Piao
   11. Wenwen Zhang
   12. Pengpeng Qu
   13. Longlong Wang
   14. Rong Xiang
   15. Yi Shi

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors studied ovarian carcinoma and identified a potential role of interleukin 20 receptor subunit alpha (IL20RA) and of IL-20 in regulating the transcoelomic metastasis of ovarian carcinoma, where IL-20 signaling in tumors is protective. This leads to the production of IL-18 and an M1 macrophage phenotype, with reduction of metastasis. The study is of interest to investigators in the area of cancer and cytokines and has potential therapeutic ramifications.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

3. Misic, a general deep learning-based method for the high-throughput cell segmentation of complex bacterial communities

   This article has 10 authors:

   1. Swapnesh Panigrahi
   2. Dorothée Murat
   3. Antoine Le Gall
   4. Eugénie Martineau
   5. Kelly Goldlust
   6. Jean-Bernard Fiche
   7. Sara Rombouts
   8. Marcelo Nöllmann
   9. Leon Espinosa
   10. Tâm Mignot

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this work, Panigrahi et. al. develop a powerful deep-learning-based cell segmentation platform (MiSiC) capable of accurately segmenting brightfield, fluorescence, and phase contrast images of bacteria cells densely packed within both homogenous and heterogeneous cell populations. This algorithm, if further optimized and disseminated to the community, will have a large impact in microbial studies in that it will allow for automated analyses of essential every aspect of bacterial cell biology, including cell morphology, cell cycle, cell-cell communications, protein localization dynamics and a variety of cellular processes using time-lapse imaging.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

4. Parallel evolution between genomic segments of seasonal human influenza viruses reveals RNA-RNA relationships

   This article has 6 authors:

   1. Jennifer E Jones
   2. Valerie Le Sage
   3. Gabriella H Padovani
   4. Michael Calderon
   5. Erik S Wright
   6. Seema S Lakdawala

   Reviewed by eLife

5. Expansion of CD10neg neutrophils and CD14+HLA-DRneg/low monocytes driving proinflammatory responses in patients with acute myocardial infarction

   This article has 6 authors:

   1. Daniela Fraccarollo
   2. Jonas Neuser
   3. Julian Möller
   4. Christian Riehle
   5. Paolo Galuppo
   6. Johann Bauersachs

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of broad interest to cardiologist and scientists studying acute myocardial infarction (AMI), especially to those focussing on the immune responses during AMI. Using combination of in vivo and in vitro model, as well as tissue from patients, the authors reveal new insights regarding the immune mechanisms during AMI, highlighting the importance of neutrophils and monocytes during the early days of its process. The findings in this paper add to the understanding of how immune mechanisms may contribute to subsequent adverse events after AMI.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors)

   Reviewed by eLife

6. T-cell receptor (TCR) signaling promotes the assembly of RanBP2/RanGAP1-SUMO1/Ubc9 nuclear pore subcomplex via PKC-θ-mediated phosphorylation of RanGAP1

   This article has 11 authors:

   1. Yujiao He
   2. Zhiguo Yang
   3. Chen-si Zhao
   4. Zhihui Xiao
   5. Yu Gong
   6. Yun-Yi Li
   7. Yiqi Chen
   8. Yunting Du
   9. Dianying Feng
   10. Amnon Altman
   11. Yingqiu Li

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     PKC-theta is known to regulate T cell activation, and this manuscript reveals a novel function of PKC-theta in the regulation of the nuclear pore complexes. The work by He and colleagues reveals that PKC-theta is recruited to the nuclear pore complex wherein it serves to regulate the assembly of key components of the RanBP2 subcomplex of the NPC, which in turn enables the translocation of AP1, NFkB and NFAT into the nucleus. However, these results need to be substantiated by additional experiments or by limiting the breath of the conclusions.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

7. Allosteric communication in class A β-lactamases occurs via cooperative coupling of loop dynamics

   This article has 13 authors:

   1. Ioannis Galdadas
   2. Shen Qu
   3. Ana Sofia F Oliveira
   4. Edgar Olehnovics
   5. Andrew R Mack
   6. Maria F Mojica
   7. Pratul K Agarwal
   8. Catherine L Tooke
   9. Francesco Luigi Gervasio
   10. James Spencer
   11. Robert A Bonomo
   12. Adrian J Mulholland
   13. Shozeb Haider

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript presents a computational study aiming to understand the allosteric signaling propagation pathway in two class-A beta-lactamases. The results of this study will be of interest to the readers in the fields of beta-lactamase, antibiotic resistance, and enzyme allostery.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewers #1, #2, and #3 agreed to share their names with the authors.)

   Reviewed by eLife

8. Asymptomatic Bordetella pertussis infections in a longitudinal cohort of young African infants and their mothers

   This article has 9 authors:

   1. Christopher J Gill
   2. Christian E Gunning
   3. William B MacLeod
   4. Lawrence Mwananyanda
   5. Donald M Thea
   6. Rachel C Pieciak
   7. Geoffrey Kwenda
   8. Zacharia Mupila
   9. Pejman Rohani

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Overall the reviewers were positive about this manuscript and the importance of this analysis being in identifying the role of asymptomatic transmitters in populations. There are some revisions that will be required and a number of areas for additional analyses and clarifications that would help the reader better put this manuscript in context.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

9. Enhanced functional detection of synaptic calcium-permeable AMPA receptors using intracellular NASPM

   This article has 6 authors:

   1. Ian Coombs
   2. Cécile Bats
   3. Craig A Sexton
   4. Dorota Studniarczyk
   5. Stuart G Cull-Candy
   6. Mark Farrant

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Here the authors identify that inclusion of intracellular NASPM can fully block Ca-permeable AMPA receptors regardless of association with auxiliary subunits. The distinction between Ca-permeable and Ca-impermeable AMPA receptors is critical to synaptic physiology, and thus these results will be of interest within the field of excitatory synaptic transmission. The data is of high quality and the experimental analysis is rigorous, but the key claim that the approach provides an unambiguous functional measure of CP-AMPAR prevalence has not been fully supported.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

10. Genetic integration of behavioural and endocrine components of the stress response

    This article has 9 authors:

    1. Thomas M Houslay
    2. Ryan L Earley
    3. Stephen J White
    4. Wiebke Lammers
    5. Andrew J Grimmer
    6. Laura M Travers
    7. Elizabeth L Johnson
    8. Andrew J Young
    9. Alastair Wilson

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This is a timely paper on the genetic integration of behavioral and physiological components of the stress response in guppies. Using evolutionary quantitative genetic approaches, the authors show that genetic variation in the cortisol stress response is associated with genetic variation in stress-related behaviors. This result suggests that physiological and behavioral responses to stress should show correlated evolution in response to natural selection, which is of interest to evolutionary biologists and for animal welfare. The reviewers pointed out several conceptual and methodological issues with the definition of the phenotypes under study and and with the definition strong genetic integration.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife