eLife

Latest preprint reviews

1. Mitochondrial phenotypes in purified human immune cell subtypes and cell mixtures

   This article has 12 authors:

   1. Shannon Rausser
   2. Caroline Trumpff
   3. Marlon A McGill
   4. Alex Junker
   5. Wei Wang
   6. Siu-Hong Ho
   7. Anika Mitchell
   8. Kalpita R Karan
   9. Catherine Monk
   10. Suzanne C Segerstrom
   11. Rebecca G Reed
   12. Martin Picard

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of broad interest to those in the field of immunometabolism, a field which has largely used the mouse as an experimental system. The descriptive work is the first of its kind to demonstrate important aspects of biological variability and hidden aspects of mitochondrial function in human immune cells.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

2. Functional architecture of pancreatic islets identifies a population of first responder cells that drive the first-phase calcium response

   This article has 9 authors:

   1. Vira Kravets
   2. JaeAnn M. Dwulet
   3. Wolfgang E. Schleicher
   4. David J. Hodson
   5. Anna M. Davis
   6. Laura Pyle
   7. Robert A. Piscopio
   8. Maura Sticco-Ivins
   9. Richard K. P. Benninger

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper provides interesting insights in the control of insulin secretion. The authors identify a subset of 'first responder' beta-cells (the cells that secrete insulin) that - possibly -influence the activity of other beta-cells. They characterise some of the properties of these cells and demonstrate the existence of a fascinating possible hierarchy within the islet. With the data consolidated by more rigorous statistical analyses, this paper will make a useful addition to the field.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

3. Cardiac pathologies in mouse loss of imprinting models are due to misexpression of H19 long noncoding RNA

   This article has 12 authors:

   1. Ki-Sun Park
   2. Beenish Rahat
   3. Hyung Chul Lee
   4. Zu-Xi Yu
   5. Jacob Noeker
   6. Apratim Mitra
   7. Connor M Kean
   8. Russell H Knutsen
   9. Danielle Springer
   10. Claudia M Gebert
   11. Beth A Kozel
   12. Karl Pfeifer

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript models a particular class of genetic lesions observed in the imprinting disorder and overgrowth syndrome, Beckwith-Wiedemann syndrome (BWS), using a highly tractable mouse model. Because more than one gene is abnormally expressed in BWS that is caused by loss of imprinting of H19 and IGF2, the authors vary the expression of both genes to investigate the source of the cardiovascular phenotypes and are able to ascribe independent heart phenotypes resulting from IGF2 overexpression and H19 loss of expression.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

4. Neural dynamics between anterior insular cortex and right supramarginal gyrus dissociate genuine affect sharing from perceptual saliency of pretended pain

   This article has 5 authors:

   1. Yili Zhao
   2. Lei Zhang
   3. Markus Rütgen
   4. Ronald Sladky
   5. Claus Lamm

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Lamm and colleagues explore brain dynamics of empathy in response to facial expressions of simulated versus genuine pain in others. Using a novel experimental fMRI task and dynamic causal modelling, the findings suggest that activation of the anterior insula for genuine pain reflects affect sharing rather than automatic responses triggered by the perceptual salience of events. The paper is of broad interest to an audience of social and affective neuroscientists interested in how humans track the emotional responses of others.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. Role of Nrp1 in controlling cortical inter-hemispheric circuits

   This article has 6 authors:

   1. Fernando Martín-Fernández
   2. Ana Bermejo-Santos
   3. Lorena Bragg-Gonzalo
   4. Carlos G Briz
   5. Esther Serrano-Saiz
   6. Marta Nieto

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Martín-Fernández et al. show that Nrp1 acts within the primary somatosensory cortex to control the refinement of axons and the topography of contralateral targeting, particularly with regards to homotopic "matching" of gradients. While this action of Nrp1 has already been discovered between cortical areas, this work elucidates its role within a cortical area, with further insight into the developmental dynamics of projection and refinement also reported. This is impactful to the field of cortical axon guidance and corpus callosum development. The data analysis is rigorous and most conclusions are justified by the data.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens

   This article has 10 authors:

   1. Robert Coukos
   2. David Yao
   3. Mateo I Sanchez
   4. Eric T Strand
   5. Meagan E Olive
   6. Namrata D Udeshi
   7. Jonathan S Weissman
   8. Steven A Carr
   9. Michael C Bassik
   10. Alice Y Ting

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Here authors present a robust, high-throughput and genome-wide strategy to identify genes that influence protein localization in individual subcellular compartments. The results profile subsets of genes that are involved in localization of tail-anchored proteins to mitochondrial and endoplasmic reticulum membranes and identify several unexpected regulators. This new tool is adaptable to studying various types of protein trafficking processes to shed light on their molecular mechanisms.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife, preLights

7. A highly conserved host lipase deacylates oxidized phospholipids and ameliorates acute lung injury in mice

   This article has 8 authors:

   1. Benkun Zou
   2. Michael Goodwin
   3. Danial Saleem
   4. Wei Jiang
   5. Jianguo Tang
   6. Yiwei Chu
   7. Robert S Munford
   8. Mingfang Lu

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The paper describes novel structures of a protein recently reported to function as a mechanosensitive ion channel. Surprisingly, the structures and functional data rather support the formerly suggested role of this protein in lipid metabolism. The paper is of relevance for ion channel field and for those interested in fatty acid metabolism.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Dual mechanisms of opioid-induced respiratory depression in the inspiratory rhythm-generating network

   This article has 4 authors:

   1. Nathan A Baertsch
   2. Nicholas E Bush
   3. Nicholas J Burgraff
   4. Jan-Marino Ramirez

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Opioids are widely used as pain killers, but present the severe side-effect of respiratory depression. The study from Baertsch et al. provides a mechanistic understanding of the actions of opioids on breathing by elucidating some of the biophysical and synaptic mechanisms by which opioids depress breathing with the goal of identifying therapeutic strategies. The data suggest that opioid-induced respiratory depression (OIRD) is due to both presynaptic hyperpolarization, and reduction of synaptic efficacy. The paper is generally well written and the data presented for the most part advances understanding of the mechanisms of OIRD at the level of central respiratory neural circuits.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

9. Linker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization

   This article has 6 authors:

   1. Pavan Choppakatla
   2. Bastiaan Dekker
   3. Erin E Cutts
   4. Alessandro Vannini
   5. Job Dekker
   6. Hironori Funabiki

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to scientists within the field of chromosome biology. The authors take advantage of the Xenopus egg cell free system and combine classical morphological analyses by immunofluorescence with chromosome conformation (Hi-C) analyses to elucidate the contribution of linker histone H1 to mitotic chromosome organization. The authors find that linker histone H1 limits the association of condensin and topoisomerase II to control chromosome length.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

10. CTP promotes efficient ParB-dependent DNA condensation by facilitating one-dimensional diffusion from parS

    This article has 8 authors:

    1. Francisco de Asis Balaguer
    2. Clara Aicart-Ramos
    3. Gemma LM Fisher
    4. Sara de Bragança
    5. Eva M Martin-Cuevas
    6. Cesar L Pastrana
    7. Mark Simon Dillingham
    8. Fernando Moreno-Herrero

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This study applies single-molecule the nanomechanical DNA manipulation together with the direct fluorescent visualization to examine the mechanism of assembly of bacterial partition/segregation complexes by the ParB protein and the ensuing condensation of parS-containing DNA. The experiments specifically show how this assembly and its DNA specificity are promoted by CTP. The authors convincingly show that following association at ParS, CTP-binding allowed ParB to diffusively spread along the DNA. ParB spreading along the DNA was in turn the prerequisite for DNA condensation mediated by this protein. Upon clarification of the ParB diffusive spreading mechanism and its activity under physiological circumstances, this study will be of broad interest to those studying protein-DNA interactions and cell division. The nanomechanical DNA condensation experiments together with the combined direct fluorescent visualization represent a helpful methodological development for future studies of this and similar systems.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife