eLife

Latest preprint reviews

1. BBB recovery after stroke by self-replenishing E-pericytes transdifferentiated from local endothelial cells

   This article has 11 authors:

   1. Li Tingbo
   2. Tu Jiaqi
   3. Gao Qingzhu
   4. Li Xuzhao
   5. Zhu Zhu
   6. Jin Yuxiao
   7. Zhang Yiyi
   8. Xie Jiongfang
   9. Zhu Peiran
   10. Zhao Bingrui
   11. Jia Jie-Min

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study aims to understand the role of endothelial cell differentiation into pericytes in the restoration of blood-brain barrier function after ischemic stroke. Identification of pericytes derived from endothelial cells and the involvement of myeloid cell-derived TGFβ1 signaling are compelling new findings, but the evidence supporting the origin and nature of these pericytes is incomplete and would benefit from more rigorous approaches demonstrating reproducibility. The work will be of interest to researchers whose work focuses on the blood-brain barrier and basic and translational stroke.

   Reviewed by eLife

2. Optogenetic control of Protein Kinase C-epsilon activity reveals its intrinsic signaling properties with spatiotemporal resolution

   This article has 17 authors:

   1. Qunxiang Ong
   2. Crystal Jing Yi Lim
   3. Yilie Liao
   4. Justin Tze-Yang Ng
   5. Ler Ting Rachel Lim
   6. Shernys Xuan Yi Koh
   7. Sher En Chan
   8. Pheobe Lee Yu Ying
   9. Huijun Lim
   10. Chen Rui Ye
   11. Loo Chien Wang
   12. Siok Ghee Ler
   13. Radoslaw M Sobota
   14. Yaw Sing Tan
   15. Gerald I Shulman
   16. Xiaoyong Yang
   17. Weiping Han

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript introduces Opto-PKCε, an optogenetic tool that enabled important findings derived from interactome and phosphoproteome studies. Light-dependent recruitment of Opto-PKCε to the plasma membrane revealed the specific phosphorylation of the insulin receptor at Thr 1160. In turn, recruitment to mitochondria led to phosphorylation of the complex I subunit NDUFS4, correlating with reduced spare respiratory capacity. The evidence supporting these conclusions is solid, although additional clarification on data analysis would further enhance readability.

   Reviewed by eLife

3. A library of lineage-specific driver lines connects developing neuronal circuits to behavior in the Drosophila Ventral Nerve Cord

   This article has 12 authors:

   1. Jelly HM Soffers
   2. Erin Beck
   3. Daniel J Sytkowski
   4. Marianne E Maughan
   5. Devarakonda Devasi
   6. Yi Zhu
   7. Beth Wilson
   8. Yu-Chieh David Chen
   9. Ted Erclik
   10. James W Truman
   11. James B Skeath
   12. Haluk Lacin

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work presents an important genetic toolkit for Drosophila neurobiologists to access and manipulate neuronal lineages during development and adulthood. The evidence supporting the fidelity of this toolkit is convincing. This work will interest Drosophila neurobiologists in general, and some of the genetic tools may be used outside the nervous system. The conceptual approaches used in this paper are likely transferable to other fields as comparable data and genomic methods are obtained.

   Reviewed by eLife

4. The general version of Hamilton’s rule

   This article has 1 author:

   1. Matthijs van Veelen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Kin selection and inclusive fitness have generated significant controversy. While not entirely new, this paper reconsiders the general form of Hamilton's rule in which benefits and costs are defined as regression coefficients, with higher-order coefficients being added to accommodate non-linear interactions. The paper is a valuable contribution to the field with convincing, systematic analysis, giving clarity to long-standing debates.

   Reviewed by eLife

5. ImPaqT - A Golden Gate-based Toolkit for Zebrafish Transgenesis

   This article has 3 authors:

   1. Saskia Hurst
   2. Christiane Dimmler
   3. Mark R Cronan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study introduces a useful toolkit for zebrafish transgenesis, significantly enhancing the flexibility and efficiency of transgene generation for immunological applications. The authors provide supporting evidence through well-designed experiments, demonstrating the toolkit's utility in generating diverse and functional transgenic lines. While the findings are solid, additional functional validation and broader comparisons to existing systems would strengthen the overall evidence base and ensure broader relevance to the zebrafish field, thereby increasing the significance of the study.

   Reviewed by eLife

6. Revealing global stoichiometry conservation architecture in cells from Raman spectral patterns

   This article has 6 authors:

   1. Ken-ichiro F Kamei
   2. Koseki J Kobayashi-Kirschvink
   3. Takashi Nozoe
   4. Hidenori Nakaoka
   5. Miki Umetani
   6. Yuichi Wakamoto

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This paper reports the fundamental finding of how Raman spectral patterns correlate with proteome profiles. The authors then go further to show that this can be used to infer global stochiometric regulation of the proteomes. These findings are likely general and the authors provide compelling evidence by analyzing bacterial and human cells but there are some suggestions provided below to make the work clearer and more accessible for it to reach a broader audience.

   Reviewed by eLife, Arcadia Science

7. Diverse Genotype-by-Weather Interactions in Switchgrass

   This article has 13 authors:

   1. Alice H MacQueen
   2. Li Zhang
   3. Samuel Pattillo Smith
   4. Jason E Bonnette
   5. Arvid R Boe
   6. Philip A Fay
   7. Felix B Fritschi
   8. David B Lowry
   9. Robert B Mitchell
   10. Francis M Rouquette
   11. Yanqi Wu
   12. Arbel Harpak
   13. Thomas E Juenger

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The study reports valuable findings on the nature of genotype-by-climate interaction, parameterised in a framework that allows integrating genetics and ecophysiological variation in switchgrass. The evidence provided is solid overall but the analysis could be improved to better support some of the claims.

   Reviewed by eLife

8. Loss of olfaction reduces caterpillar performance and increases susceptibility to a natural enemy

   This article has 8 authors:

   1. Qi Wang
   2. Yufei Jia
   3. Hans M Smid
   4. Berhane T Weldegergis
   5. Liana O Greenberg
   6. Maarten Jongsma
   7. Marcel Dicke
   8. Alexander Haverkamp

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important manuscript investigates the role of olfactory cues in Pieris brassicae larvae, focusing on their interactions with the host plant Brassica oleracea and the parasitoid wasp Cotesia glomerata. The authors' demonstration that impaired olfactory perception reduces caterpillar performance and increases susceptibility to parasitism is solid. These findings highlight the ecological significance of olfaction in mediating feeding behavior and predator avoidance in herbivorous insects.

   Reviewed by eLife

9. DANCE: An open-source analysis pipeline and low-cost hardware to quantify aggression and courtship in Drosophila

   This article has 11 authors:

   1. R Sai Prathap Yadav
   2. Paulami Dey
   3. Faizah Ansari
   4. Tanvi Kottat
   5. P Pallavi Prabhu
   6. Manohar Vasam
   7. Shrinivas Ayyangar
   8. Swathi Bhaskar S
   9. Krishnananda Prabhu
   10. Monalisa Ghosh
   11. Pavan Agrawal

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a valuable open-source and cost-effective method for automating the quantification of male aggression and courtship in Drosophila melanogaster. The work as presented provides solid evidence that the use of the behavioral setup that the authors designed - using readily available laboratory equipment and standardised high-performing classifiers they developed using existing software packages - accurately and reliably characterises social behavior in Drosophila. The work will be of interest to Drosophila neurobiologists and particularly to those working on male social behaviors.

   Reviewed by eLife

10. Mac/Lac-tosylceramide regulates intestinal homeostasis and secretory cell fate commitment by facilitating Notch signaling

    This article has 9 authors:

    1. Kebei Tang
    2. Xuewen Li
    3. Jiulong Hu
    4. Jingyuan Shi
    5. Yumei Li
    6. Yansu Chen
    7. Chang Yin
    8. Fengchao Wang
    9. Rongwen Xi

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This important study provides solid evidence that glucosylceramide synthase (GlcT), a rate-limiting enzyme for glycosphingolipid (GSL) production, plays a role in the differentiation of intestinal cells. Mutations in GlcT compromise Notch signaling in the Drosophila intestinal stem cell lineage resulting in the formation of enteroendocrine tumors, and preliminary data suggests that a homolog of glucosylceramide synthase also influences Notch signaling in the mammalian intestine. While the outstanding strengths of the initial genetic and downstream pathway analyses are noted, there are weaknesses in the data regarding the potential role of this pathway in Delta trafficking. Nevertheless, this study opens the way for future mechanistic studies addressing how specific lipids modulate Notch signalling activity.

    Reviewed by eLife