eLife

Latest preprint reviews

1. The role of adolescent lifestyle habits in biological aging: A prospective twin study

   This article has 6 authors:

   1. Anna Kankaanpää
   2. Asko Tolvanen
   3. Aino Heikkinen
   4. Jaakko Kaprio
   5. Miina Ollikainen
   6. Elina Sillanpää

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This paper provides evidence that an unhealthy lifestyle during adolescence accelerates epigenetic age in adulthood, and that these associations are largely explained by the effect of shared genetic influences. The main strengths of this valuable paper are the relatively large sample size, longitudinal assessment of lifestyle factors, and sophisticated statistical analyses. The paper is methodologically compelling and will be of interest for a broad audience, including individuals working on methylation, epidemiology, and/or ageing.

   Reviewed by eLife

2. Spatiotemporal organisation of human sensorimotor beta burst activity

   This article has 7 authors:

   1. Catharina Zich
   2. Andrew J Quinn
   3. James J Bonaiuto
   4. George O'Neill
   5. Lydia C Mardell
   6. Nick S Ward
   7. Sven Bestmann

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors use high spatial resolution MEG in humans to link two important components (time - transient bursts, space - waves) of neural sensorimotor dynamics by investigating how transient beta bursts propagate in the brain. The authors find two directions of propagating waves during beta bursts. The work links two fundamental aspects of neural dynamics which may yield new insights into the origins of sensorimotor behavior, with wide appeal to neuroscientists and clinicians. The reviewers considered the methodological work largely sound, although concerns were raised by the reviewers to what extent the travelling waves correspond to underlying neural activity or reflect the generative nature of field potentials.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

3. Cellular features of localized microenvironments in human meniscal degeneration: a single-cell transcriptomic study

   This article has 7 authors:

   1. Weili Fu
   2. Sijie Chen
   3. Runze Yang
   4. Chen Li
   5. Haoxiang Gao
   6. Jian Li
   7. Xuegong Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to researchers studying meniscus homeostasis and knee osteoarthritis. It uncovers distinct subtypes of cell populations in inner and outer part of human meniscus using single-cell RNA sequencing. In particular, this work further identifies how alterations in meniscal cell populations may contribute to inflammation and osteoarthritis and thus serves as a resource paper for the field.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

4. Identification of orphan ligand-receptor relationships using a cell-based CRISPRa enrichment screening platform

   This article has 7 authors:

   1. Dirk H Siepe
   2. Lukas T Henneberg
   3. Steven C Wilson
   4. Gaelen T Hess
   5. Michael C Bassik
   6. Kai Zinn
   7. K Christopher Garcia

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript reports the development of a proteo-genomic screening methodology to identify protein-protein interactions between secreted proteins and their cell surface receptors. The authors use a CRISPRa-based approach to overexpress membrane proteins in cells and then use magnetic cell sorting to identify receptors that bind candidate ligands. This approach leads to the identification of several novel interaction pairs that are validated biochemically, including receptor tyrosine phosphatase ligands and other interactions with implications for immune system function. The work is of interest to a wide variety of fields including biochemistry and signaling.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. RNA sequence to structure analysis from comprehensive pairwise mutagenesis of multiple self-cleaving ribozymes

   This article has 5 authors:

   1. Jessica M Roberts
   2. James D Beck
   3. Tanner B Pollock
   4. Devin P Bendixsen
   5. Eric J Hayden

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors provide a summary of single and double mutants in five self-cleaving ribozymes using next-generation sequencing. They dissect their data in terms of epistasis effects, which provides a new angle to understanding ribozyme function. In principle, this allows conclusions to be drawn on bases involved in pairs and in catalysis that have the potential to be of use to the field, although there is also a series of technical weaknesses that should be addressed.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

6. The meningeal transcriptional response to traumatic brain injury and aging

   This article has 9 authors:

   1. Ashley C Bolte
   2. Daniel A Shapiro
   3. Arun B Dutta
   4. Wei Feng Ma
   5. Katherine R Bruch
   6. Michael A Kovacs
   7. Ana Royo Marco
   8. Hannah E Ennerfelt
   9. John R Lukens

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Identification of transcriptional phenotypes driven by TBI across meningeal immune cell subsets and the effect of aging on these responses is an important and not well-defined area in the field. Multiple complementary and high-end approaches are taken to demonstrate the long-lasting effects that TBI drives in the brain and support the main findings of the manuscript. This manuscript will be of interest to readers in the field(s) of neuroimmunology, aging, and traumatic brain injury.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

7. Arginase 1 is a key driver of immune suppression in pancreatic cancer

   This article has 20 authors:

   1. Rosa E Menjivar
   2. Zeribe C Nwosu
   3. Wenting Du
   4. Katelyn L Donahue
   5. Hanna S Hong
   6. Carlos Espinoza
   7. Kristee Brown
   8. Ashley Velez-Delgado
   9. Wei Yan
   10. Fatima Lima
   11. Allison Bischoff
   12. Padma Kadiyala
   13. Daniel Salas-Escabillas
   14. Howard C Crawford
   15. Filip Bednar
   16. Eileen Carpenter
   17. Yaqing Zhang
   18. Christopher J Halbrook
   19. Costas A Lyssiotis
   20. Marina Pasca di Magliano

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Menjivar et al. identify a previously unrecognized role of myeloid cell Arginase1 (Arg1) activity in shaping the anti-tumor immune response in pancreatic ductal adenocarcinoma (PDAC). The proposed therapeutic combination is a new approach for pancreatic cancer, with an enhanced response to immune therapy upon arginase inhibition.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Bumblebees retrieve only the ordinal ranking of foraging options when comparing memories obtained in distinct settings

   This article has 10 authors:

   1. Cwyn Solvi
   2. Yonghe Zhou
   3. Yunxiao Feng
   4. Yuyi Lu
   5. Mark Roper
   6. Li Sun
   7. Rebecca J Reid
   8. Lars Chittka
   9. Andrew B Barron
   10. Fei Peng

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors investigate what type and degree of information (either absolute, relative, or a weighted combination of both) is used by bumblebees when retrieving the value of an item. There is recent evidence in humans and birds that suggests that these organisms use a combination of absolute memories and remembering of subjective ranking in these tasks. The authors conclude that bumblebees indeed use remembered ranking, but that they seem not to be able to retain (or at least utilise) absolute property information for very long. The absence of relevant work in invertebrates would make this study a potentially valuable addition to the scientific literature.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

9. Rabphilin 3A binds the N-peptide of SNAP-25 to promote SNARE complex assembly in exocytosis

   This article has 4 authors:

   1. Tianzhi Li
   2. Qiqi Cheng
   3. Shen Wang
   4. Cong Ma

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Li et al. use biochemical binding analysis to explore the role of Rabphilin 3A in dense-core vesicle exocytosis in neuroendocrine PC12 cells and in an in vitro SNARE assembly assay. They propose that the Rph3A binding to SNAP25 pre-structures the protein to efficiently assemble with Syntaxin and VAMP2, and thus, promoting the vesicle docking and priming process. This work will be of interest to scientists studying the molecular basis of synaptic vesicle release.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. Sporulation activated via σ ^W protects Bacillus from a Tse1 peptidoglycan hydrolase T6SS effector

    This article has 4 authors:

    1. Alicia I. Pérez-Lorente
    2. Carlos Molina-Santiago
    3. Antonio de Vicente
    4. Diego Romero

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This manuscript will be of interest to readers in the field of microbiology, particularly those interested in bacterial interactions, secretion systems, and stress responses. It identifies the molecules and mechanisms that explain a competitive interaction between two soil-dwelling bacterial species. The data support most of the conclusions of the manuscript, but some controls are lacking and some of the interpretations are not fully justified by the experiments shown.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife