eLife

Latest preprint reviews

1. Evolved bacterial resistance to the chemotherapy gemcitabine modulates its efficacy in co-cultured cancer cells

   This article has 8 authors:

   1. Serkan Sayin
   2. Brittany Rosener
   3. Carmen G Li
   4. Bao Ho
   5. Olga Ponomarova
   6. Doyle V Ward
   7. Albertha JM Walhout
   8. Amir Mitchell

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental work advances our understanding of how bacteria evolve to resist drugs used for cancer treatment and how this could potentially affect drug efficacy and treatment outcome. The data were collected and analyzed using a solid methodology and can be used as a starting point for functional studies of the interaction between microbiome interactions and cancer drug treatment. The findings will be of broad interest to microbiologists and organismal biologists interested in the role of microbiomes in drug responses.

   Reviewed by eLife

2. Mechanical basis and topological routes to cell elimination

   This article has 4 authors:

   1. Siavash Monfared
   2. Guruswami Ravichandran
   3. José Andrade
   4. Amin Doostmohammadi

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this work, Monfared et al. construct a useful three-dimensional phase-field model for cell layers and use this to investigate the link of extrusion events to defects in cellular arrangement. The extension of existing 2D phase field models to three dimensions is an important contribution of this paper. Here the model is used to study the importance of cell-cell and cell-substrate interaction in extrusion from cell monolayers. Their claim that extrusion events can be distinctly linked to defects in nematic and hexatic orders in the monolayer need to be better justified to be fully convincing.

   Reviewed by eLife

3. Muscle calcium stress cleaves junctophilin1, unleashing a gene regulatory program predicted to correct glucose dysregulation

   This article has 9 authors:

   1. Eshwar R Tammineni
   2. Lourdes Figueroa
   3. Carlo Manno
   4. Disha Varma
   5. Natalia Kraeva
   6. Carlos A Ibarra
   7. Amira Klip
   8. Sheila Riazi
   9. Eduardo Rios

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Junctophilin has been traditionally known as a structural anchor to keep excitation-contraction proteins in place for healthy contractile function of skeletal muscle. Here the authors provide an interesting and important role in skeletal muscle for Junctophilin, where it translocates to the nuclei and influences gene transcription. The authors provide convincing evidence for a novel role of junctophilin beyond its structural role as a regulator of gene expression.

   Reviewed by eLife

4. T follicular helper 17 (Tfh17) cells are superior for immunological memory maintenance

   This article has 28 authors:

   1. Xin Gao
   2. Kaiming Luo
   3. Diya Wang
   4. Yunbo Wei
   5. Yin Yao
   6. Jun Deng
   7. Yang Yang
   8. Qunxiong Zeng
   9. Xiaoru Dong
   10. Le Xiong
   11. Dongcheng Gong
   12. Lin Lin
   13. Kai Pohl
   14. Shaoling Liu
   15. Yu Liu
   16. Lu Liu
   17. Thi HO Nguyen
   18. Lilith F Allen
   19. Katherine Kedzierska
   20. Yanliang Jin
   21. Mei-Rong Du
   22. Wanping Chen
   23. Liangjing Lu
   24. Nan Shen
   25. Zheng Liu
   26. Ian A Cockburn
   27. Wenjing Luo
   28. Di Yu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The enrichment of Tfh17 cells in Tfh cell central memory compartment and the dominance of Tfh17 cell population and the Tfh17 transcriptional signature in circulating Tfh cells at the memory phase are nicely demonstrated, and may well be helpful for understanding the heterogeneity of memory Tfh cells and potentially providing clues for vaccine design. The in vitro differentiation system for mouse Tfh cells also provides a strategy for others to build upon in dissection of Tfh cell development and function.

   Reviewed by eLife

5. Structures of RecBCD in complex with phage-encoded inhibitor proteins reveal distinctive strategies for evasion of a bacterial immunity hub

   This article has 6 authors:

   1. Martin Wilkinson
   2. Oliver J Wilkinson
   3. Connie Feyerherm
   4. Emma E Fletcher
   5. Dale B Wigley
   6. Mark S Dillingham

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study addresses the ways in which bacteriophages antagonize or coopt the DNA restriction and/or recombination functions of the bacterial RecBCD helicase-nuclease. The evidence from both biochemistry and structural biology showing convergent evolution is convincing.

   Reviewed by eLife

6. Scleraxis-lineage cells are required for tendon homeostasis and their depletion induces an accelerated extracellular matrix aging phenotype

   This article has 4 authors:

   1. Antonion Korcari
   2. Anne EC Nichols
   3. Mark R Buckley
   4. Alayna E Loiselle

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental work advances our understanding of the cellular and molecular changes of the aged tendon. The evidence supporting the conclusion is convincing, using a DTR-based ScxLin cell depletion model along with state-of-art proteomic and scRNA-seq analyses. This paper is of potential interest to scientists and physicians who study the mechanisms of the tendon aging process.

   Reviewed by eLife

7. STAT3 promotes RNA polymerase III-directed transcription by controlling the miR-106a-5p/TP73 axis

   This article has 9 authors:

   1. Cheng Zhang
   2. Shasha Zhao
   3. Huan Deng
   4. Shihua Zhang
   5. Juan Wang
   6. Xiaoye Song
   7. Deen Yu
   8. Yue Zhang
   9. Wensheng Deng

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The author arrive at the convincing conclusion that STAT3 expression promotes TFIIIB assembly through miR-106A-5p-mediated inhibition of TP73 expression, thereby increasing Pol III transcription, which contributes to enhanced cell proliferation. The data are very good and clearly support the proposed model.

   Reviewed by eLife

8. Structures of human dynein in complex with the lissencephaly 1 protein, LIS1

   This article has 4 authors:

   1. Janice M Reimer
   2. Morgan E DeSantis
   3. Samara L Reck-Peterson
   4. Andres E Leschziner

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents the cryo-EM structure of the dynein regulator Lis1 bound to human dynein providing important insight into how these two proteins interact. The evidence supporting the claims of the authors is overall convincing though it requires some minor re-analysis. The work will be of interest to researchers working with motor proteins and neurodevelopmental disorders as it helps rationalize how mutations in Lis1 or dynein lead to disease.

   Reviewed by eLife

9. Ribosomal RNA (rRNA) sequences from 33 globally distributed mosquito species for improved metagenomics and species identification

   This article has 10 authors:

   1. Cassandra Koh
   2. Lionel Frangeul
   3. Hervé Blanc
   4. Carine Ngoagouni
   5. Sébastien Boyer
   6. Philippe Dussart
   7. Nina Grau
   8. Romain Girod
   9. Jean-Bernard Duchemin
   10. Maria-Carla Saleh

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript generates a valuable new genetic resource for studying mosquitos and the pathogens that they carry. For 33 species of mosquitoes, the authors have sequenced and assembled the ribosomal RNA, which will dramatically improve the power of RNA sequencing in mosquitoes.

   Reviewed by eLife

10. Myosin II regulatory light chain phosphorylation and formin availability modulate cytokinesis upon changes in carbohydrate metabolism

    This article has 10 authors:

    1. Francisco Prieto-Ruiz
    2. Elisa Gómez-Gil
    3. Rebeca Martín-García
    4. Armando Jesús Pérez-Díaz
    5. Jero Vicente-Soler
    6. Alejandro Franco
    7. Teresa Soto
    8. Pilar Pérez
    9. Marisa Madrid
    10. José Cansado

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      Force generation by the myosin motor plays an important role during cell division. Myosin activity is regulated by phosphorylation, which activates myosin in animals but was thought to inactivate it in yeast. In this valuable study, the authors use a combination of convincing approaches to show that under some growth conditions, dependent on the carbon source of the growth medium, phosphorylation becomes essential for myosin function.

    Reviewed by eLife