eLife

Latest preprint reviews

1. Retinal microvascular and neuronal pathologies probed in vivo by adaptive optical two-photon fluorescence microscopy

   This article has 9 authors:

   1. Qinrong Zhang
   2. Yuhan Yang
   3. Kevin J Cao
   4. Wei Chen
   5. Santosh Paidi
   6. Chun-hong Xia
   7. Richard H Kramer
   8. Xiaohua Gong
   9. Na Ji

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors developed a two-photon fluorescence microscope coupled with adaptive optics (AO-2PFM), allowing in vivo imaging of the mouse retinal structure and function. This new imaging system will be important for exploring normal retinal physiology and pathological alterations in retinal disease models.

   Reviewed by eLife

2. Competition between lysogenic and sensitive bacteria is determined by the fitness costs of the different emerging phage-resistance strategies

   This article has 3 authors:

   1. Olaya Rendueles
   2. Jorge AM de Sousa
   3. Eduardo PC Rocha

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The overarching question of the manuscript is important and the findings inform the patterns and mechanisms of phage-mediated bacterial competition, with implications for microbial evolution and antimicrobial resistance. The evidence in the manuscript is, however, still incomplete and some of the conclusions made are not supported by the data. This manuscript would additionally be strengthened by a clearer narrative, to enable readers to more easily extract the key message this paper wants to convey.

   Reviewed by eLife

3. Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis

   This article has 6 authors:

   1. Kunli Zhao
   2. Kohichi Matsunaga
   3. Kouichi Mizuno
   4. Hao Wang
   5. Katsuhide Okunishi
   6. Tetsuro Izumi

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is a well-done study to understand how Rab27 and its effectors regulate insulin secretion. The present work examines the relative hierarchy of exophilin-8 and melanophilin using single vs double knockouts and rescue experiments to show that melanophilin functions downstream of and potentially redundantly from Exo8. Imaging and protein co-localization studies were done in a rigorous way. The data are solid, and some additional data will make the work fully compelling. Overall this is an important study that sheds new light on the regulation of insulin granule exocytosis.

   Reviewed by eLife

4. Gαq-PKD/PKCμ signal regulating the nuclear export of HDAC5 to induce the IκB expression and limit the NF-κB-mediated inflammatory response essential for early pregnancy

   This article has 15 authors:

   1. Yufei Jiang
   2. Yan He
   3. Songting Liu
   4. Gaizhen Li
   5. Dunjin Chen
   6. Wenbo Deng
   7. Ping Li
   8. Ying Zhang
   9. Jinxiang Wu
   10. Jianing Li
   11. Longmei Wang
   12. Jiajing Lin
   13. Haibin Wang
   14. Shuangbo Kong
   15. Guixiu Shi

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents a valuable finding on the role and function of the Gaq axis on the inflammatory response during decidualization essential for early pregnancy. The evidence supporting the claims of the authors is solid, although some of the methodology and data interpretation require further clarification and justification. The work will be of interest to reproductive biologists and clinicians.

   Reviewed by eLife

5. Identification of quiescent FOXC2+ spermatogonial stem cells in adult mammals

   This article has 20 authors:

   1. Zhipeng Wang
   2. Cheng Jin
   3. Pengyu Li
   4. Yiran Li
   5. Jielin Tang
   6. Zhixin Yu
   7. Tao Jiao
   8. Jinhuan Ou
   9. Han Wang
   10. Dingfeng Zou
   11. Mengzhen Li
   12. Xinyu Mang
   13. Jun Liu
   14. Yan Lu
   15. Kai Li
   16. Ning Zhang
   17. Jia Yu
   18. Shiying Miao
   19. Linfang Wang
   20. Wei Song

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study reports that Foxc2+ cells in the testis represent the quiescent spermatogonial stem cells (SSCs). The data supporting this claim are solid. The finding is of great significance to reproductive and stem-cell biology as male fertility depends on the fine balance between self-renewal and differentiation activities of the male germline stem cells, i.e., SSCs.

   Reviewed by eLife

6. Non-invasive real-time genomic monitoring of the critically endangered kākāpō

   This article has 9 authors:

   1. Lara Urban
   2. Allison K Miller
   3. Daryl Eason
   4. Deidre Vercoe
   5. Megan Shaffer
   6. Shaun P Wilkinson
   7. Gert-Jan Jeunen
   8. Neil J Gemmell
   9. Andrew Digby

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This work presents important findings regarding the use of soil environmental DNA for non-invasive monitoring of the endangered kākāpō parrot population in New Zealand. The approach based on sequence analysis is convincing but comparisons to established methods are lacking. The tools presented in this study are innovative and will be relevant to those working with environmental DNA and the conservation of biodiversity.

   Reviewed by eLife

7. Dynamic compartmentalization of the pro-invasive transcription factor NHR-67 reveals a role for Groucho in regulating a proliferative-invasive cellular switch in C. elegans

   This article has 13 authors:

   1. Taylor N Medwig-Kinney
   2. Brian A Kinney
   3. Michael AQ Martinez
   4. Callista Yee
   5. Sydney S Sirota
   6. Angelina A Mullarkey
   7. Neha Somineni
   8. Justin Hippler
   9. Wan Zhang
   10. Kang Shen
   11. Christopher Hammell
   12. Ariel M Pani
   13. David Q Matus

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable data study presents convincing data that expression of the C. elegans transcription factor NHR-67 is sufficient to drive an invasive fate, and that the alternative proliferative fate is associated with NHR-67 transcriptional down-regulation. While the observation that NHR-67 forms punctae associated with transcriptional repressors in non-invasive cells is intriguing, the work does not yet established a clear link between the formation and dissolution of NHR-67 condensates with the activation of downstream genes that NHR-67 is actively repressing. The work will be of interest to developmental biologists studying transcriptional control of cell fate specification in animals, especially once issues around the functional significance of the NHR-67 contiaining punctae are resolved.

   Reviewed by eLife

8. De Novo Multi-Omics Pathway Analysis Designed for Prior Data Independent Inference of Cell Signaling Pathways

   This article has 4 authors:

   1. Katri Vaparanta
   2. Johannes A.M. Merilahti
   3. Veera K. Ojala
   4. Klaus Elenius

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment:

     This manuscript describes development of a new algorithm for integrative analysis of multi-omics data. This work should be of potential interest to scientists performing bioinformatic pathway discovery in multi-omic datasets especially those that relate to signaling.

   Reviewed by eLife

9. The ER tether VAPA is required for proper cell motility and anchors ER-PM contact sites to focal adhesions

   This article has 9 authors:

   1. Hugo Siegfried
   2. Georges Farkouh
   3. Rémi Le Borgne
   4. Catherine Pioche-Durieu
   5. Thaïs De Azevedo Laplace
   6. Agathe Verraes
   7. Lucien Daunas
   8. Jean-Marc Verbavatz
   9. Mélina L Heuzé

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript presents valuable findings that bring together two important topics in cell biology: the function of membrane contact sites and cell migration. The authors describe a role of the ER tether protein VAP-A in focal adhesion dynamics and cell motility. Although the authors present solid evidence to support some of the main claims of the paper, some of the other claims would benefit from stronger experimental support. Nonetheless, this paper will be of interest to those cell biologists and biophysicists working on adhesion, migration, and membrane contact site biology.

   Reviewed by eLife

10. Bone marrow Adipoq-lineage progenitors are a major cellular source of M-CSF that dominates bone marrow macrophage development, osteoclastogenesis, and bone mass

    This article has 10 authors:

    1. Kazuki Inoue
    2. Yongli Qin
    3. Yuhan Xia
    4. Jie Han
    5. Ruoxi Yuan
    6. Jun Sun
    7. Ren Xu
    8. Jean X Jiang
    9. Matthew B Greenblatt
    10. Baohong Zhao

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This paper is of interest for skeletal biologists studying bone marrow stem/progenitor cells and bone remodeling. Using Adipoq-Cre-driven conditional deletion of Csf1 that encodes M-CSF and reanalyzing publicly available scRNAseq data, the authors recognize a subpopulation of bone marrow cells (i.e. AdipoQ-lineage progenitors) as an important source of M-CSF. The authors found that M-CSF production from these bone marrow cells influences the development of macrophages and osteoclasts as well as bone mass, including the bone loss associated with estrogen deficiency. This is a clearly written and nicely presented study that has potential to offer important new information regarding the source of M-CSF in the bone marrow.

    Reviewed by eLife