eLife

Latest preprint reviews

1. Ketamine’s rapid antidepressant effects are mediated by Ca2+-permeable AMPA receptors

   This article has 8 authors:

   1. Anastasiya Zaytseva
   2. Evelina Bouckova
   3. McKennon J Wiles
   4. Madison H Wustrau
   5. Isabella G Schmidt
   6. Hadassah Mendez-Vazquez
   7. Latika Khatri
   8. Seonil Kim

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This paper addresses an important clinical concern which is how the antidepressant ketamine exerts its effects acts rapidly. The authors suggest the reason is that ketamine increases glutamatergic transmission in the hippocampus. The strengths are the data are mostly very good, and the limitations are a lack of compelling evidence that the hippocampus is the location where effects occur, as well as several other issues.

   Reviewed by eLife

2. Cyclic AMP binding to a universal stress protein in Mycobacterium tuberculosis is essential for viability

   This article has 8 authors:

   1. Arka Banerjee
   2. Moubani Chakraborty
   3. Suruchi Sharma
   4. Ruchi Chaturvedi
   5. Avipsa Bose
   6. Priyanka Biswas
   7. Amit Singh
   8. Sandhya S. Visweswariah

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study will be of interest to those working on mycobacterial signal transduction. A combination of experiments provides convincing evidence to show how universal stress proteins bind to cAMP and function by direct sequestration of the second messenger. Although the methods, data and analyses broadly support the conclusions, the main claims are only partially supported and can be strengthened through further analytic approaches.

   Reviewed by eLife

3. Rapid cell type-specific nascent proteome labeling in Drosophila

   This article has 7 authors:

   1. Stefanny Villalobos-Cantor
   2. Ruth M Barrett
   3. Alec F Condon
   4. Alicia Arreola-Bustos
   5. Kelsie M Rodriguez
   6. Michael S Cohen
   7. Ian Martin

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Villalobos-Cantor et. al. describe a new technique for cell-type specific in vivo labeling of nascent peptides, which they call POPPi. POPPi is based on sequence-independent incorporation of the puromycin analog OPP into an elongating peptide, which also simultaneously terminates the growing peptide. To achieve cell-type-specific labeling, the authors used an OPP derivative, PhAc-OPP, as the labeling substrate. The method is potentially interesting but needs further characterization to be able to assess its use.

   Reviewed by eLife

4. Promoting axon regeneration by inhibiting RNA N6-methyladenosine demethylase ALKBH5

   This article has 8 authors:

   1. Dong Wang
   2. Tiemei Zheng
   3. Songlin Zhou
   4. Mingwen Liu
   5. Yaobo Liu
   6. Xiaosong Gu
   7. Susu Mao
   8. Bin Yu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study combines a screen of known N6-methyladenine (m6A)-dependent RNA modifying factors to identify ALKBH5 as critical in crush injury response. They demonstrate through gain and loss of function an effect on ALKBH5 m6A-dependent Lpin2 mRNA stability during injury-induced axon regeneration in both dorsal root ganglia nerve and optic nerve regeneration. The results provide new insight into the role of RNA modification on neural injury. However, the limitations of the experimental design on the conclusions drawn require additional consideration. With additional control experiments and further consideration of the limitations, the paper will provide a link between N6-methyladenine and neurotrauma.

   Reviewed by eLife

5. Myofibroblast senescence promotes arrhythmogenic remodeling in the aged infarcted rabbit heart

   This article has 19 authors:

   1. Brett C Baggett
   2. Kevin R Murphy
   3. Elif Sengun
   4. Eric Mi
   5. Yueming Cao
   6. Nilufer N Turan
   7. Yichun Lu
   8. Lorraine Schofield
   9. Tae Yun Kim
   10. Anatoli Y Kabakov
   11. Peter Bronk
   12. Zhilin Qu
   13. Patrizia Camelliti
   14. Patrycja Dubielecka
   15. Dmitry Terentyev
   16. Federica del Monte
   17. Bum-Rak Choi
   18. John Sedivy
   19. Gideon Koren

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study describes important results and convincing evidence linking myofibroblast senescence in the aged heart with a pro-arrhythmogenic phenotype. This is in turn related to higher mortality after myocardial infarction in the aged rabbit heart. These constitute important empiric as opposed to detailed findings. They nevertheless will be of interest to clinician scientists studying cardiac function and disease.

   Reviewed by eLife

6. Differences in the inflammatory proteome of East African and Western European adults and associations with environmental and dietary factors

   This article has 13 authors:

   1. Godfrey S Temba
   2. Nadira Vadaq
   3. Vesla Kullaya
   4. Tal Pecht
   5. Paolo Lionetti
   6. Duccio Cavalieri
   7. Joachim L Schultze
   8. Reginald Kavishe
   9. Leo AB Joosten
   10. Andre J van der Ven
   11. Blandina T Mmbaga
   12. Mihai G Netea
   13. Quirijn de Mast

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Non-communicable diseases (NCDs) are rising rapidly in urbanizing populations in many parts of the developing world, including in sub-Saharan Africa. Temba and colleagues show important evidence that healthy Tanzanians display a pro-inflammatory phenotype with enrichment of specific immune-metabolic pathways. Dood-derived metabolites were identified as an important driver of inflammation-related molecules. These findings provide solid evidence that the dietary transition that occurs in urbanizing areas in sub-Saharan Africa may contribute significantly to the increased incidence of non communicable diseases in this part of the world.

   Reviewed by eLife

7. The prolactin receptor scaffolds Janus kinase 2 via co-structure formation with phosphoinositide-4,5-bisphosphate

   This article has 9 authors:

   1. Raul Araya-Secchi
   2. Katrine Bugge
   3. Pernille Seiffert
   4. Amalie Petry
   5. Gitte W Haxholm
   6. Kresten Lindorff-Larsen
   7. Stine Falsig Pedersen
   8. Lise Arleth
   9. Birthe B Kragelund

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important interdisciplinary study substantially advances our understanding of the prolactin receptor interactions with the membrane lipids and the effect of these interactions on cell signaling. The authors use a combination of state-of-the-art NMR structural analysis, simulations, and cellular assays to provide compelling experimental evidence for protein complexes being regulated by IDR-membrane interactions. The work will be of broad interest to structural biologists and biochemists, and the results presented herein are likely relevant for other non-tyrosine kinase receptors.

   Reviewed by eLife

8. Antagonistic role of the BTB-zinc finger transcription factors Chinmo and Broad-Complex in the juvenile/pupal transition and in growth control

   This article has 5 authors:

   1. Sílvia Chafino
   2. Panagiotis Giannios
   3. Jordi Casanova
   4. David Martín
   5. Xavier Franch-Marro

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study demonstrates that the transcription factor Chinmo is a master regulator that maintains larval growth and development as part of the metamorphic gene network in Drosophila. Chinmo does so in part by regulating Broad expression in imaginal tissues (exemplified in the wing disc) and in a Broad-independent manner in other larval tissues such as the salivary gland. Finally, they demonstrate that the role of Chinmo in promoting larval development is conserved between holometabolous insects and hemimetabolous insects, which lack a pupal stage. The data were collected and analyzed using solid and validated methodology and will be of interest to a broad audience including those interested in development and evolution.

   Reviewed by eLife

9. Force propagation between epithelial cells depends on active coupling and mechano-structural polarization

   This article has 13 authors:

   1. Artur Ruppel
   2. Dennis Wörthmüller
   3. Vladimir Misiak
   4. Manasi Kelkar
   5. Irène Wang
   6. Philippe Moreau
   7. Adrien Méry
   8. Jean Révilloud
   9. Guillaume Charras
   10. Giovanni Cappello
   11. Thomas Boudou
   12. Ulrich S Schwarz
   13. Martial Balland

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Using surface micropatterning, optical activation, and theoretical analysis, the authors provide compelling evidence that adjacent cells actively propagate mechanical stress in epithelial tissues. The response of the receiver cell is active and enhanced when the principal stress direction is perpendicular to the orientation of actin fibers. This work is important and a must-read for everybody wanting to understand tissue mechanics.

   Reviewed by eLife

10. Neural circuit-wide analysis of changes to gene expression during deafening-induced birdsong destabilization

    This article has 5 authors:

    1. Bradley M Colquitt
    2. Kelly Li
    3. Foad Green
    4. Robert Veline
    5. Michael S Brainard

    This article has been curated by 1 group:

    • Curated by eLife

      **eLife assessment
      **
      This is an important study that uses the song system in a bird model to understand the transcriptional mechanisms underlying neuronal adaptations to sensory deprivation. The manuscript offers compelling data in support of their hypothesis that these transcriptional changes are related to song plasticity. The work will be of interest to biologists who study neuronal plasticity mechanisms.

    Reviewed by eLife