eLife

Latest preprint reviews

1. Regulation of different phases of AMPA receptor intracellular transport by 4.1N and SAP97

   This article has 5 authors:

   1. Caroline Bonnet
   2. Justine Charpentier
   3. Natacha Retailleau
   4. Daniel Choquet
   5. Françoise Coussen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study by Bonnet et al addresses the question of how AMPA receptor numbers at the synapse are regulated during basal conditions and during chemically induced Long Term Potentiation. Specifically, the study aims to determine the molecular mechanisms that contribute to the intracellular trafficking of AMPA receptors and determine their insertion into the synaptic plasma membrane. Using compelling methodology, the authors dissect the distinct roles of two proteins that bind to the C-terminal domain of the AMPA receptor subunit GluA1: 4.1N and SAP97. The findings will be of interest to anyone trying to understand molecular events contributing to synaptic plasticity in health and disease, and more broadly, the method could be adapted for tracking intracellular movements of a wide range of proteins.

   Reviewed by eLife

2. Selection of HIV-1 for resistance to fifth-generation protease inhibitors reveals two independent pathways to high-level resistance

   This article has 12 authors:

   1. Ean Spielvogel
   2. Sook-Kyung Lee
   3. Shuntai Zhou
   4. Gordon J Lockbaum
   5. Mina Henes
   6. Amy Sondgeroth
   7. Klajdi Kosovrasti
   8. Ellen A Nalivaika
   9. Akbar Ali
   10. Nese Kurt Yilmaz
   11. Celia A Schiffer
   12. Ronald Swanstrom

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript significantly advances our understanding of the development of drug resistance in the HIV-1 protease. The paper addresses the fundamental relationship between resistance mutations and inhibitor structure and will be useful in the design and development of the next generation of inhibitors.

   Reviewed by eLife

3. Study of efficacy and longevity of immune response to third and fourth doses of COVID-19 vaccines in patients with cancer: A single arm clinical trial

   This article has 23 authors:

   1. Astha Thakkar
   2. Kith Pradhan
   3. Benjamin Duva
   4. Juan Manuel Carreno
   5. Srabani Sahu
   6. Victor Thiruthuvanathan
   7. Sean Campbell
   8. Sonia Gallego
   9. Tushar D Bhagat
   10. Johanna Rivera
   11. Gaurav Choudhary
   12. Raul Olea
   13. Maite Sabalza
   14. Lauren C Shapiro
   15. Matthew Lee
   16. Ryann Quinn
   17. Ioannis Mantzaris
   18. Edward Chu
   19. Britta Will
   20. Liise-anne Pirofski
   21. Florian Krammer
   22. Amit Verma
   23. Balazs Halmos

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study evaluates the immunogenicity of 3rd and 4th doses of SARS-CoV2 vaccinations in patients with cancer. Their study is notable in that neutralization of Omicron was absent in all patients after the third dose but increased to 33% after the fourth dose. With the definitions and patient population better described, this paper would be of interest to those studying the effects of repeated COVID boosters on Omicron immunity.

   Reviewed by eLife

4. A model of hippocampal replay driven by experience and environmental structure facilitates spatial learning

   This article has 2 authors:

   1. Nicolas Diekmann
   2. Sen Cheng

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This paper proposes a new computational model for replay that is biologically realistic and accounts for a number of important phenomena in hippocampal replay. This is an important study with implications for multiple subfields. Whilst the majority of claims are convincingly supported by the data, simulation analyses for some crucial aspects of replay literature are currently incomplete.

   Reviewed by eLife

5. Retinal microvascular and neuronal pathologies probed in vivo by adaptive optical two-photon fluorescence microscopy

   This article has 9 authors:

   1. Qinrong Zhang
   2. Yuhan Yang
   3. Kevin J Cao
   4. Wei Chen
   5. Santosh Paidi
   6. Chun-hong Xia
   7. Richard H Kramer
   8. Xiaohua Gong
   9. Na Ji

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors developed a two-photon fluorescence microscope coupled with adaptive optics (AO-2PFM), allowing in vivo imaging of the mouse retinal structure and function. This new imaging system will be important for exploring normal retinal physiology and pathological alterations in retinal disease models.

   Reviewed by eLife

6. Competition between lysogenic and sensitive bacteria is determined by the fitness costs of the different emerging phage-resistance strategies

   This article has 3 authors:

   1. Olaya Rendueles
   2. Jorge AM de Sousa
   3. Eduardo PC Rocha

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The overarching question of the manuscript is important and the findings inform the patterns and mechanisms of phage-mediated bacterial competition, with implications for microbial evolution and antimicrobial resistance. The evidence in the manuscript is, however, still incomplete and some of the conclusions made are not supported by the data. This manuscript would additionally be strengthened by a clearer narrative, to enable readers to more easily extract the key message this paper wants to convey.

   Reviewed by eLife

7. Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis

   This article has 6 authors:

   1. Kunli Zhao
   2. Kohichi Matsunaga
   3. Kouichi Mizuno
   4. Hao Wang
   5. Katsuhide Okunishi
   6. Tetsuro Izumi

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is a well-done study to understand how Rab27 and its effectors regulate insulin secretion. The present work examines the relative hierarchy of exophilin-8 and melanophilin using single vs double knockouts and rescue experiments to show that melanophilin functions downstream of and potentially redundantly from Exo8. Imaging and protein co-localization studies were done in a rigorous way. The data are solid, and some additional data will make the work fully compelling. Overall this is an important study that sheds new light on the regulation of insulin granule exocytosis.

   Reviewed by eLife

8. Gαq-PKD/PKCμ signal regulating the nuclear export of HDAC5 to induce the IκB expression and limit the NF-κB-mediated inflammatory response essential for early pregnancy

   This article has 15 authors:

   1. Yufei Jiang
   2. Yan He
   3. Songting Liu
   4. Gaizhen Li
   5. Dunjin Chen
   6. Wenbo Deng
   7. Ping Li
   8. Ying Zhang
   9. Jinxiang Wu
   10. Jianing Li
   11. Longmei Wang
   12. Jiajing Lin
   13. Haibin Wang
   14. Shuangbo Kong
   15. Guixiu Shi

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents a valuable finding on the role and function of the Gaq axis on the inflammatory response during decidualization essential for early pregnancy. The evidence supporting the claims of the authors is solid, although some of the methodology and data interpretation require further clarification and justification. The work will be of interest to reproductive biologists and clinicians.

   Reviewed by eLife

9. Identification of quiescent FOXC2+ spermatogonial stem cells in adult mammals

   This article has 20 authors:

   1. Zhipeng Wang
   2. Cheng Jin
   3. Pengyu Li
   4. Yiran Li
   5. Jielin Tang
   6. Zhixin Yu
   7. Tao Jiao
   8. Jinhuan Ou
   9. Han Wang
   10. Dingfeng Zou
   11. Mengzhen Li
   12. Xinyu Mang
   13. Jun Liu
   14. Yan Lu
   15. Kai Li
   16. Ning Zhang
   17. Jia Yu
   18. Shiying Miao
   19. Linfang Wang
   20. Wei Song

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study reports that Foxc2+ cells in the testis represent the quiescent spermatogonial stem cells (SSCs). The data supporting this claim are solid. The finding is of great significance to reproductive and stem-cell biology as male fertility depends on the fine balance between self-renewal and differentiation activities of the male germline stem cells, i.e., SSCs.

   Reviewed by eLife

10. Non-invasive real-time genomic monitoring of the critically endangered kākāpō

    This article has 9 authors:

    1. Lara Urban
    2. Allison K Miller
    3. Daryl Eason
    4. Deidre Vercoe
    5. Megan Shaffer
    6. Shaun P Wilkinson
    7. Gert-Jan Jeunen
    8. Neil J Gemmell
    9. Andrew Digby

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This work presents important findings regarding the use of soil environmental DNA for non-invasive monitoring of the endangered kākāpō parrot population in New Zealand. The approach based on sequence analysis is convincing but comparisons to established methods are lacking. The tools presented in this study are innovative and will be relevant to those working with environmental DNA and the conservation of biodiversity.

    Reviewed by eLife