eLife

Latest preprint reviews

1. Microtubule-mediated GLUT4 trafficking is disrupted in insulin-resistant skeletal muscle

   This article has 13 authors:

   1. Jonas R Knudsen
   2. Kaspar W Persson
   3. Carlos Henriquez-Olguin
   4. Zhencheng Li
   5. Nicolas Di Leo
   6. Sofie A Hesselager
   7. Steffen H Raun
   8. Janne R Hingst
   9. Raphaël Trouillon
   10. Martin Wohlwend
   11. Jørgen FP Wojtaszewski
   12. Martin AM Gijs
   13. Thomas Elbenhardt Jensen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This paper provides strong evidence for the important point that microtubule function is required for the proper localization of Glut4 glucose transporters in an insulin responsive compartment. This membrane localization is required in turn for effective translocation of Glut4 to the muscle cell surface in response to the hormone.

   Reviewed by eLife

2. Precise temporal control of neuroblast migration through combined regulation and feedback of a Wnt receptor

   This article has 7 authors:

   1. Erik S Schild
   2. Shivam Gupta
   3. Clément Dubois
   4. Euclides E Fernandes Póvoa
   5. Marie-Anne Félix
   6. Andrew Mugler
   7. Hendrik C Korswagen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This paper deals with an important unsolved problem in developmental biology of how cells execute their dynamics at the right time. The study combines compelling quantitative single cell and single transcript experiments with genetic perturbations and computational modelling and provides important insights into how the timing of transcription is regulated. The work would be strengthened by better integration of modeling and data.

   Reviewed by eLife

3. Projected long-term effects of colorectal cancer screening disruptions following the COVID-19 pandemic

   This article has 9 authors:

   1. Pedro Nascimento de Lima
   2. Rosita van den Puttelaar
   3. Anne I Hahn
   4. Matthias Harlass
   5. Nicholson Collier
   6. Jonathan Ozik
   7. Ann G Zauber
   8. Iris Lansdorp-Vogelaar
   9. Carolyn M Rutter

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study uses two well-established colorectal cancer models to estimate the potential impact of disruptions in screening caused by the COVID-19 pandemic. By dividing the population into separate cohorts based on age and pre-pandemic screening status, the authors provide convincing evidence for the adverse impact of delays in screening, switching regimens, and screening discontinuation. The finding that discontinuation has a much greater impact on screening-associated gains in life expectancy than shorter-term delays or switching of regimens suggests that access-related barriers to screening resumption may lead to the worsening of current disparities.

   Reviewed by eLife

4. Differential ripple propagation along the hippocampal longitudinal axis

   This article has 2 authors:

   1. Roberto De Filippo
   2. Dietmar Schmitz

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is an interesting study investigating the propagation dynamics of ripples recorded from the dorsal hippocampus of mice using an open-access dataset. Findings may have theoretical and practical implications for the study and manipulation of sharp-wave ripples, a main oscillatory event underlying memory consolidation. While the strength of evidence is solid and claims broadly supported, there are some points requiring additional analysis to clarify issues regarding the anatomical axes involved and to reinforce mechanistic insights.

   Reviewed by eLife

5. Integrated signaling and transcriptome analysis reveals Src family kinase individualities and novel pathways controlled by their constitutive activity

   This article has 8 authors:

   1. Nikolaos Koutras
   2. Vasileios Morfos
   3. Kyriakos Konnaris
   4. Adamantia Kouvela
   5. Athanasios-Nasir Shaukat
   6. Constantinos Stathopoulos
   7. Vassiliki Stamatopoulou
   8. Konstantina Nika

   This article has been curated by 1 group:

   • Curated by eLife

     **eLife assessment
     **
     This study presents valuable findings on how the ectopic expression of the Lck protein tyrosine kinase in Chronic Lymphocytic Leukemias (CLL) contributes to malignant transformation. The evidence supporting the claims of the authors is solid, although the use of gene editing to directly explore the functions unique to LCK in a CLL model would increase the appeal of the work.

   Reviewed by eLife

6. Structural and functional properties of a plant NRAMP-related aluminum transporter

   This article has 8 authors:

   1. Karthik Ramanadane
   2. Márton Liziczai
   3. Dragana Markovic
   4. Monique S Straub
   5. Gian T Rosalen
   6. Anto Udovcic
   7. Raimund Dutzler
   8. Cristina Manatschal

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study provides valuable insight into the diversity within the NRAMP superfamily of transporters. Evidence of divalent metal ion transport and the structure (obtained without added metal ions) are convincing. However, molecular insight into Al3+ recognition and transport is incomplete, and the work would be strengthened by the determination of a metal-bound structure or additional experiments (such as molecular dynamics simulations or quantitative Al3+ binding/transport assays) to support the proposed Al3+ binding site. The work will be of interest to structural biologists and biophysicists studying NRAMP transporters.

   Reviewed by eLife

7. Changes in transmission of Enterovirus D68 (EV-D68) in England inferred from seroprevalence data

   This article has 7 authors:

   1. Margarita Pons-Salort
   2. Ben Lambert
   3. Everlyn Kamau
   4. Richard Pebody
   5. Heli Harvala
   6. Peter Simmonds
   7. Nicholas C Grassly

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors use data from 3 cross-sectional age-stratified serosurveys on Enterovirus D68 from England between 2006 and 2017 to examine the transmission dynamics of this pathogen in this setting. Understanding these dynamics, including how it changes over time, may help uncover potential changes in the transmissibility of the virus. While the topic is relevant, interpretation of the results challenging largely due to the great uncertainty around how to interpret the serological (serostatus) data, and the impact this has on the inferences made. We ask the authors to perform some additional analyses and to provide more intuition to understand some of the key findings of this analysis.

   Reviewed by eLife

8. Local generation and efficient evaluation of numerous drug combinations in a single sample

   This article has 2 authors:

   1. Vlad Elgart
   2. Joseph Loscalzo

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this manuscript, a method to test a large number of drug combinations in a single cell culture sample is presented. The strength of the evidence lies in their multiple experiments with different combinations of agents. The paper suggests that results from this application are feasible and the methodology could be applied in other laboratories to use drug combinations for defined outcomes.

   Reviewed by eLife

9. Stimulation of the catalytic activity of the tyrosine kinase Btk by the adaptor protein Grb2

   This article has 5 authors:

   1. Laura M Nocka
   2. Timothy J Eisen
   3. Anthony T Iavarone
   4. Jay T Groves
   5. John Kuriyan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors' finding that Btk kinase activation relies on specific interactions with the Grb2 scaffold protein, including for recruitment into signaling clusters on membranes, is an unexpected finding that will potentially be of broad interest. The authors make a case for reinterpretation of the "Saraste dimer" of Btk as a signaling entity and assign roles to the component domains in the Src module in Btk activation, but the data provided are not yet fully convincing for this scenario.

   Reviewed by eLife

10. A descending inhibitory mechanism of nociception mediated by an evolutionarily conserved neuropeptide system in Drosophila

    This article has 8 authors:

    1. Izumi Oikawa
    2. Shu Kondo
    3. Kao Hashimoto
    4. Akiho Yoshida
    5. Megumi Hamajima
    6. Hiromu Tanimoto
    7. Katsuo Furukubo-Tokunaga
    8. Ken Honjo

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This is a very interesting and important study that convincingly demonstrates a descending pathway for the control of nociception in non-mammalian organisms.

    Reviewed by eLife