eLife

Latest preprint reviews

1. Multiple modes of cholesterol translocation in the human Smoothened receptor

   This article has 4 authors:

   1. Prateek D Bansal
   2. Maia Kinnebrew
   3. Rajat Rohatgi
   4. Diwakar Shukla

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this useful study, the authors conducted a set of computational and experimental investigations of the mechanism of cholesterol transport in the smoothened (SMO) protein. The computational component integrated multiple state-of-the-art approaches such as adaptive sampling, free energy simulations, and Markov state modeling, providing support for the proposed mechanistic model, which is also consistent with the experimental mutagenesis data. However, substantial revisions are needed for the discussion of the computational results and interpretation of the literature to provide a more balanced and accurate perspective on cholesterol-mediated SMO regulation. In the current form, therefore, the strength of evidence of the study is considered incomplete.

   Reviewed by eLife

2. MIRO1 controls energy production and proliferation of vascular smooth muscle cells

   This article has 15 authors:

   1. Lan Qian
   2. Olha M Koval
   3. Benney T Endoni
   4. Denise Juhr
   5. Colleen S Stein
   6. Chantal Allamargot
   7. Li-Hsien Lin
   8. Deng-Fu Guo
   9. Kamal Rahmouni
   10. Antentor O Hinton
   11. E Dale Abel
   12. Ryan L Boudreau
   13. Jennifer Streeter
   14. William H Thiel
   15. Isabella M Grumbach

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The findings are important, as they identify MIRO1 as a central regulator linking mitochondrial positioning and respiratory chain function to VSMC proliferation, neointima formation, and human vasoproliferative disease. Overall, the strength of evidence is convincing, with comprehensive in vivo and in vitro data, including human cells and added bioenergetic analyses, that broadly support the main claims despite some remaining limitations in mechanistic and mitochondrial assays.

   Reviewed by eLife

3. Dynamic assembly of malate dehydrogenase-citrate synthase multienzyme complex in the mitochondria

   This article has 5 authors:

   1. Joy Omini
   2. Inga Krassovskaya
   3. Taiwo Dele-Osibanjo
   4. Connor Pedersen
   5. Toshihiro Obata

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study reports a dynamic association/dissociation between malate dehydrogenase (MDH1) and citrate synthase (CIT1) in Saccharomyces cerevisiae under different metabolic conditions that control TCA pathway flux rate. The research question is timely, the use of the NanoBiT split-luciferase system to monitor protein-protein interactions is innovative, and the significance of the findings is valuable. However, the strength of evidence needed to support the conclusions was found to be incomplete based on a lack of critical control and mechanistic experiments.

   Reviewed by eLife

4. Faroese Whole Genomes Provide Insight into Ancestry and Recent Selection

   This article has 13 authors:

   1. Iman Hamid
   2. Ólavur Mortensen
   3. Alba Refoyo-Martinez
   4. Leivur N Lydersen
   5. Anne-Katrin Emde
   6. Melissa Hendershott
   7. Katrin D Apol
   8. Guðrið Andorsdóttir
   9. Jonas Meisner
   10. Kaja A Wasik
   11. Fernando Racimo
   12. Stephane E Castel
   13. Noomi O Gregersen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This paper presents an analysis of demography and selection from whole-genome sequencing of 40 Faroese, with data that are useful beyond the study region. Much of the analysis is solid, but a more fine-scale analysis of demographic history could have led to more interesting findings. In addition, there are concerns about the selection analyses, given the special nature of the studied population and sampling scheme. Finally, lack of data availability limits the broader value of the paper.

   Reviewed by eLife

5. Acquired resistance to sotorasib in KRASG12C mutant NSCLC is vulnerable to PI3K-mTOR pathway inhibition mediated by 4E-BP1 regulator of cap-dependent translation

   This article has 15 authors:

   1. Ismail M Meraz
   2. Shuhong Wu
   3. Yi Xu
   4. Lihui Gao
   5. Meng Feng
   6. Chenghui Ren
   7. Renduo Song
   8. Ran Zhang
   9. Qi Wang
   10. Yuanxin Xi
   11. Sung Yun Jung
   12. Jing Wang
   13. Bingliang Fang
   14. Mourad Majidi
   15. Jack A Roth

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors investigate mechanisms of acquired resistance (AR) to KRAS-G12C inhibitors (sotorasib) in non-small cell lung cancer, proposing that resistance arises from signaling rewiring rather than additional mutations. While the study addresses a valuable clinical question, it is limited by several weaknesses in experimental rigor, data interpretation, and presentation, meaning the strength of evidence is incomplete

   Reviewed by eLife

6. Systematic characterization of site-specific proline hydroxylation using hydrophilic interaction chromatography and mass spectrometry

   This article has 7 authors:

   1. Hao Jiang
   2. Jimena Druker
   3. James W Wilson
   4. Dalila Bensaddek
   5. Jason R Swedlow
   6. Sonia Rocha
   7. Angus I Lamond

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The study presents a valuable resource of proline hydroxylation proteins for molecular biology studies in oxygen-sensing and cell signaling with the characterization of Repo-man proline hydroxylation site. The evidence supporting the claim of the authors is solid, although further clarification of the overall efficiency of the HILIC analysis, the specificity/sensitivity of immonium ion analysis, as well as quantification of proline hydroxylation identifications will be helpful. The work will be of interest to researchers studying post-translational modification, oxygen sensing, and cell signaling.

   Reviewed by eLife

7. Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol

   This article has 6 authors:

   1. Samuel Bru
   2. Lydie Michaillat Mayer
   3. Geun-Don Kim
   4. Danye Qiu
   5. Henning J Jessen
   6. Andreas Mayer

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides fundamental insights into eukaryotic phosphate homeostasis by demonstrating how yeast vacuoles dynamically regulate cytosolic phosphate levels. The conclusions are convincing, supported by an elegant combination of in vitro assays and in vivo measurements. This study will be of interest to cell biologists, particularly for those who are working in the field of phosphate metabolism.

   Reviewed by eLife

8. Structural insights into the recruitment of viral Type 2 IRES to ribosomal preinitiation complex for protein synthesis

   This article has 2 authors:

   1. Deepakash Das
   2. Tanweer Hussain

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript offers valuable structural and mechanistic insights into the structure and assembly of the Type II internal ribosome entry site (IRES) from encephalomyocarditis virus (EMCV) and the translation initiation complex, revealing a direct interaction between the IRES and the 40S ribosomal subunit. While a solid cryo-EM method was used, enhancing the overall resolution or adding complementary biochemical data would further improve the clarity and impact of this study. This manuscript will attract researchers in cap-independent translation, host-pathogen interactions, and virology.

   Reviewed by eLife

9. Task structure tailors the geometry of neural representations in human lateral prefrontal cortex

   This article has 4 authors:

   1. Apoorva Bhandari
   2. Haley Keglovits
   3. Defne Buyukyazgan
   4. David Badre

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a valuable finding on the representational structure of task encoding in the prefrontal cortex. The evidence supporting the claims of the authors is solid, representing an impressive data collection effort and best-practice fMRI analyses. However, at least including visual regions as a control and controlling for behavioral differences in the task in representation analyses would have strengthened the study. The work will be of interest to cognitive neuroscientists interested in the neural basis of cognitive control.

   Reviewed by eLife

10. Dynamics of mesoscale brain network during visual discrimination learning revealed by chronic, large-scale single-unit recording

    This article has 5 authors:

    1. Tian-Yi Wang
    2. Chengcong Feng
    3. Chengyao Wang
    4. Chi Ren
    5. Zhengtuo Zhao

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This study presents experiments suggesting intriguing mesoscale reorganization of functional connectivity across distributed cortical and subcortical circuits during learning. The approach is technically impressive and the results are potentially of valuable significance. The authors have also made clear effort to address concerns in revision. However, the strength of evidence remains incomplete. Acquisition of data from additional animals in the primary experiment could bolster these findings.

    Reviewed by eLife