eLife

Latest preprint reviews

1. Metabolic disruption impairs ribosomal protein levels, resulting in enhanced aminoglycoside tolerance

   This article has 2 authors:

   1. Rauf Shiraliyev
   2. Mehmet A Orman

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The findings of this study are valuable as they challenge the dogma regarding the link between lowered bacterial metabolism and tolerance to aminoglycosides. The authors propose that the well-known tolerance to AG of mutants such as those of complexes I and II is not due to a decrease in the proton motive force and thus antibiotic uptake. The results presented here are convincing.

   Reviewed by eLife

2. Activating SRC/MAPK signaling via 5-HT1A receptor contributes to the effect of vilazodone on improving thrombocytopenia

   This article has 17 authors:

   1. Ling Zhou
   2. Chengyang Ni
   3. Ruixue Liao
   4. Xiaoqin Tang
   5. Taian Yi
   6. Mei Ran
   7. Miao Huang
   8. Rui Liao
   9. Xiaogang Zhou
   10. Dalian Qin
   11. Long Wang
   12. Feihong Huang
   13. Xiang Xie
   14. Ying Wan
   15. Jiesi Luo
   16. Yiwei Wang
   17. Jianming Wu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents a rather valuable finding that vilazodone can restore the normal platelet level through regulating 5-HT1A receptor. The evidence supporting the claims of the authors is solid, although inclusion of more cell lines and more detailed analysis of the results would have strengthened the study. The work will be of interest to scientists working in the field of thrombocytopenia.

   Reviewed by eLife

3. Polysaccharide breakdown products drive degradation-dispersal cycles of foraging bacteria through changes in metabolism and motility

   This article has 11 authors:

   1. Astrid Katharina Maria Stubbusch
   2. Johannes M Keegstra
   3. Julia Schwartzman
   4. Sammy Pontrelli
   5. Estelle E Clerc
   6. Samuel Charlton
   7. Roman Stocker
   8. Cara Magnabosco
   9. Olga T Schubert
   10. Martin Ackermann
   11. Glen G D'Souza

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript is a valuable contribution to our understanding of foraging behaviors in marine bacteria. The authors present a conceptual model for how a marine bacterial species consumes an abundant polysaccharide. Using experiments in microfluidic devices and through measurements of motility and gene expression, the authors offer convincing evidence that the degradation products of polysaccharide digestion can stimulate motility.

   Reviewed by eLife

4. Mapping variation in the morphological landscape of human cells with optical pooled CRISPRi screening

   This article has 8 authors:

   1. Ramon Lorenzo D. Labitigan
   2. Adrian L. Sanborn
   3. Cynthia V. Hao
   4. Caleb K. Chan
   5. Nathan M. Belliveau
   6. Eva M. Brown
   7. Mansi Mehrotra
   8. Julie A. Theriot

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this important study by Theriot et al., the authors utilize an impressive set of innovative approaches to conduct a CRISPRi pooled screen in human cells using large-scale microscopy screen data. They leverage an improved barcoding approach to identify genes targeted in specific cells and examine the effects on cell morphology using high-dimensional phenotypic analysis. The method and data presented are compelling.

   Reviewed by eLife

5. Metabolite profiling of human renal cell carcinoma reveals tissue-origin dominance in nutrient availability

   This article has 21 authors:

   1. Keene L Abbott
   2. Ahmed Ali
   3. Bradley I Reinfeld
   4. Amy Deik
   5. Sonu Subudhi
   6. Madelyn D Landis
   7. Rachel A Hongo
   8. Kirsten L Young
   9. Tenzin Kunchok
   10. Christopher S Nabel
   11. Kayla D Crowder
   12. Johnathan R Kent
   13. Maria Lucia L Madariaga
   14. Rakesh K Jain
   15. Kathryn E Beckermann
   16. Caroline A Lewis
   17. Clary B Clish
   18. Alexander Muir
   19. W Kimryn Rathmell
   20. Jeffrey Rathmell
   21. Matthew G Vander Heiden

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study provides an important finding that the local abundance of metabolites impacts the biology of the tumor microenvironment by utilizing kidney tumors from patients and adjacent normal tissues. The evidence supporting the claims of the authors is convincing. The work will of interest to the research community working on metabolism and kidney cancer especially.

   Reviewed by eLife

6. Visuo-motor updating in individuals with heightened autistic traits

   This article has 2 authors:

   1. Antonella Pomè
   2. Eckart Zimmermann

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study shows that a high autism quotient in neurotypical adults is associated with suboptimal motor planning and visual updating after eye movements, suggesting a disrupted efference copy mechanism. The implication is that abnormal visuomotor updating may contribute to sensory overload - a key symptom in autism spectrum disorder. The evidence presented is convincing, with few limitations, and should be of broad interest to neuroscientists at large.

   Reviewed by eLife

7. Knockdown of PHOX2B in the retrotrapezoid nucleus reduces the central CO2 chemoreflex in rats

   This article has 4 authors:

   1. Silvia Cardani
   2. Tara A Janes
   3. William Betzner
   4. Silvia Pagliardini

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study utilizes a viral-mediated short hairpin RNA (shRNA) approach to investigate in a novel way the role of the wild-type PHOX2B transcription factor expressed in critical chemosensory neurons in the brainstem retrotrapezoid nucleus (RTN) region for maintaining normal CO2 chemoreflex control of breathing in adult rats. The convincing results show blunted ventilation during elevated inhaled CO2 (hypercapnia) with knockdown of PHOX2B, accompanied by a reduced expression of Gpr4 and Task2 mRNA for the proposed RTN neuron proton sensor proteins GPR4 and TASK2. These results indicate that maintained expression of wild-type PHOX2B affects respiratory control in adult animals, complementing previous studies showing that PHOX2B-expressing RTN neurons may be critical for chemosensory control throughout the lifespan, and with implications for neurological disorders involving the RTN, which will be of interest to neuroscientists studying respiratory neurobiology as well as the neurodevelopmental control of motor behavior.

   Reviewed by eLife

8. Allosteric modulation of the CXCR4:CXCL12 axis by targeting receptor nanoclustering via the TMV-TMVI domain

   This article has 25 authors:

   1. Eva M García-Cuesta
   2. Pablo Martínez
   3. Karthik Selvaraju
   4. Gabriel Ulltjärn
   5. Adrián Miguel Gómez Pozo
   6. Gianluca D'Agostino
   7. Sofia Gardeta
   8. Adriana Quijada-Freire
   9. Patricia Blanco Gabella
   10. Carlos Roca
   11. Daniel del Hoyo
   12. Rodrigo Jiménez-Saiz
   13. Alfonso García-Rubia
   14. Blanca Soler Palacios
   15. Pilar Lucas
   16. Rosa Ayala-Bueno
   17. Noelia Santander Acerete
   18. Yolanda Carrasco
   19. Carlos Oscar Sorzano
   20. Ana Martinez
   21. Nuria E Campillo
   22. Lasse D Jensen
   23. Jose Miguel Rodriguez Frade
   24. César Santiago
   25. Mario Mellado

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is an important study that describes an elegant modelling driven approach to design of allosteric antagonists for CXCR4 that have a selective effect on receptor nanocluster formation, cell polarisation and chemotaxis, but spare binding of CXCL12 to the receptor and inhibition of adenylate cyclase. This enables selective targeting of processes dependent upon cell polarisation and chemotaxis without impacting signalling effects and may avoid some of the toxicity associated with antagonists that target CXCL12 binding and thus block all CXCR4 signalling. The revised manuscript offers convincing evidence to support the claims. The modelling work is better described and additional data has been presented that better illustrates the unique features of the new antagonist. The in vivo studies in the zebrafish model open a path to studies in mammalian models.

   Reviewed by eLife

9. A Myelin Map of Trunk Folds in the Elephant Trigeminal Nucleus

   This article has 11 authors:

   1. Noémie Reveyaz
   2. Undine Schneeweiß
   3. Olivia Heise
   4. Ben Gerhardt
   5. Andreea M Gui
   6. Lena V Kaufmann
   7. Jette Alfken
   8. Jakob Reichmann
   9. Tim Salditt
   10. Thomas Hildebrandt
   11. Michael Brecht

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable study uses neuroanatomical techniques to investigate somatosensory projections from the elephant trunk to the brainstem. Given its unique specializations, understanding how the elephant trunk is represented within the brain is of general interest to evolutionary and comparative neuroscientists. The authors present solid evidence for the existence of a novel isomorphism in which the folds of the trunk are mapped onto the trigeminal nucleus; however, due to their unusual structure, some uncertainty remains about the identification and anatomical organization of nuclei within the elephant brainstem.

   Reviewed by eLife

10. Antigenic drift and subtype interference shape A(H3N2) epidemic dynamics in the United States

    This article has 26 authors:

    1. Amanda C Perofsky
    2. John Huddleston
    3. Chelsea L Hansen
    4. John R Barnes
    5. Thomas Rowe
    6. Xiyan Xu
    7. Rebecca Kondor
    8. David E Wentworth
    9. Nicola Lewis
    10. Lynne Whittaker
    11. Burcu Ermetal
    12. Ruth Harvey
    13. Monica Galiano
    14. Rodney Stuart Daniels
    15. John W McCauley
    16. Seiichiro Fujisaki
    17. Kazuya Nakamura
    18. Noriko Kishida
    19. Shinji Watanabe
    20. Hideki Hasegawa
    21. Sheena G Sullivan
    22. Ian G Barr
    23. Kanta Subbarao
    24. Florian Krammer
    25. Trevor Bedford
    26. Cécile Viboud

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This paper explores the relationships among evolutionary and epidemiological quantities in influenza, and presents fundamental findings that substantially advance our understanding of the drivers of influenza epidemics. The authors use a rich set of data sources to gather and analyze compelling evidence on the roles of genetic distance, other influenza dynamics and epidemiological indicators in predicting influenza epidemics. The central findings highlight the significant influence of genetic distance on A(H3N2) virus epidemiology and emphasize the role of A(H1N1) virus incidence in shaping A(H3N2) epidemics, suggesting subtype interference as a key factor. This paper also makes relevant data available to the research community.

    Reviewed by eLife