eLife

Latest preprint reviews

1. Optogenetic control of a GEF of RhoA uncovers a signaling switch from retraction to protrusion

   This article has 4 authors:

   1. Jean De Seze
   2. Maud Bongaerts
   3. Benoit Boulevard
   4. Mathieu Coppey

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study combines compelling experiments with optogenetic actuation and convincing theory to understand how signalling proteins control the switch between cell protrusion and retraction, two essential processes in single cell migration. The authors examine the importance of the basal concentration and recruitment dynamics of a guanine exchange factor (GEF) on the activity of the downstream effectors RhoA and Cdc42, which control retraction and protrusion. The experimental and theoretical evidence provides a model of RhoA's involvement in both protrusion and retraction and shows that these complex processes are highly dependent on the concentration and activity dynamics of the components.

   Reviewed by eLife

2. A 2-hydroxybutyrate-mediated feedback loop regulates muscular fatigue

   This article has 14 authors:

   1. Brennan J Wadsworth
   2. Marina Leiwe
   3. Eleanor A Minogue
   4. Pedro P Cunha
   5. Viktor Engman
   6. Carolin Brombach
   7. Christos Asvestis
   8. Shiv K Sah-Teli
   9. Emilia Marklund
   10. Peppi Koivunen
   11. Jorge L Ruas
   12. Helene Rundqvist
   13. Johanna T Lanner
   14. Randall S Johnson

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The work by Johnson and co-workers has identified an important role of 2-Hydroxybutyrate in skeletal muscle oxidative capacity in the early stages of exercise. Mechanistically, they show convincing data to support a role of 2-Hydroxybutyrate in the regulation of BCAA metabolism via SIRT4, ADP-Ribosylation, and CEBP. However, whether this is the sole mechanism and if these translate to longer exercise training regimes requires future experiments.

   Reviewed by eLife

3. Structure-guided mutagenesis of OSCAs reveals differential activation to mechanical stimuli

   This article has 4 authors:

   1. Sebastian Jojoa-Cruz
   2. Adrienne E Dubin
   3. Wen-Hsin Lee
   4. Andrew B Ward

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The manuscript seeks to dissect the molecular underpinnings of poke and stretch activation in OSCA channels. While the structural and functional experiments are well done, and the authors present some important data, the reviewers identified weaknesses in experimental design and interpretation that render the data incomplete in supporting some of the main conclusions of the paper. Nevertheless, this work will be of interest to those working in the fields of mechanosensation, sensory biology, and ion channels.

   Reviewed by eLife

4. Genetic associations between circulating immune cells and periodontitis highlight the prospect of systemic immunoregulation in periodontal care

   This article has 12 authors:

   1. Xinjian Ye
   2. Yijing Bai
   3. Mengjun Li
   4. Yuhang Ye
   5. Yitong Chen
   6. Bin Liu
   7. Yuwei Dai
   8. Shan Wang
   9. Weiyi Pan
   10. Zhiyong Wang
   11. Yingying Mao
   12. Qianming Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this fundamental study, the authors analyzed associations between circulating immune cells and periodontitis. Convincing evidence identifies three immune cell types related to periodontitis, which substantially advances our understanding of periodontitis.

   Reviewed by eLife

5. GABRD promotes the progression of breast cancer through CDK1-dependent cell cycle regulation

   This article has 12 authors:

   1. Qingyao Shang
   2. Fei Ren
   3. Kexin Feng
   4. Chenxuan Yang
   5. Shuangtao Zhao
   6. Jiaxiang Liu
   7. Xiyu Kang
   8. Jiaxian Yue
   9. Ruixuan Zhang
   10. Xiangzhi Meng
   11. Xiang Wang
   12. Xin Wang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents a useful finding on the role of GABRD and its downstream target CDK1 in the progression of breast cancer. The evidence supporting the claims of the authors is somewhat incomplete and the elaboration of the mechanistic details on GABARD/CDK1 regulation would have strengthened the study. The work will be of interest to clinicians and biologists working on breast cancer.

   Reviewed by eLife

6. Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism

   This article has 5 authors:

   1. Ivan Kulich
   2. Julia Schmid
   3. Anastasia Teplova
   4. Linlin Qi
   5. Jiří Friml

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental study addresses the earliest events that enable plant roots to reorient growth in response to gravity. Compelling molecular and cell biological data establish that plasma membrane localization of the LAZY or NEGATIVE GRAVITROPIC RESPONSE OF ROOTS (NGR) protein family is required for rapid and polar redirection of D6 protein kinase, an activator of the PIN3 auxin transporter. This work complements and extends recent publications on the NGR family in gravity sensing (PMID: 37741279 and PMID: 37561884). Collectively these papers advance our understanding of rapid plant gravity sensing and response.

   Reviewed by eLife

7. Allosteric activation of the co-receptor BAK1 by the EFR receptor kinase initiates immune signaling

   This article has 5 authors:

   1. Henning Mühlenbeck
   2. Yuko Tsutsui
   3. Mark A Lemmon
   4. Kyle W Bender
   5. Cyril Zipfel

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript reports important in vitro biochemical and in planta experiments to study the receptor activation mechanism of plant membrane receptor kinase complexes with non-catalytic intracellular kinase domains. Several lines of evidence convincingly show that one such putative pseudokinase, the immune receptor EFR achieves an active conformation following phosphorylation by a co-receptor kinase, and then in turn activates the co-receptor kinase allosterically to enable it to phosphorylate down-stream signaling components. This manuscript will be of interest to scientists focusing on cell signalling and allosteric regulation.

   Reviewed by eLife

8. BMP2 and BMP7 cooperate with H3.3K27M to promote quiescence and invasiveness in pediatric diffuse midline gliomas

   This article has 27 authors:

   1. Paul Huchede
   2. Swann Meyer
   3. Clément Berthelot
   4. Maud Hamadou
   5. Adrien Bertrand-Chapel
   6. Andria Rakotomalala
   7. Line Manceau
   8. Julia Tomine
   9. Nicolas Lespinasse
   10. Paul Lewandowski
   11. Martine Cordier-Bussat
   12. Laura Broutier
   13. Aurélie Dutour
   14. Isabelle Rochet
   15. Jean-Yves Blay
   16. Cyril Degletagne
   17. Valéry Attignon
   18. Angel Montero-Carcaboso
   19. Marion Le Grand
   20. Eddy Pasquier
   21. Alexandre Vasiljevic
   22. Pascale Gilardi-Hebenstreit
   23. Samuel Meignan
   24. Pierre Leblond
   25. Vanessa Ribes
   26. Erika Cosset
   27. Marie Castets

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable study examines whether the BMP signaling pathway has a role in H3.3K27M DMG tumors, regardless of the presence of ACRVR1 activating mutations. The authors provide solid evidence that BMP2/7 synergizes with H3.3K27M to induce a transcriptomic rewiring associated with a quiescent but invasive cell state. Although this work could be further enhanced by the inclusion of additional models, the study overall points to BMP2/7 as a potential target for future therapies in this deadly cancer.

   Reviewed by eLife

9. Dynamic 1D search and processive nucleosome translocations by RSC and ISW2 chromatin remodelers

   This article has 12 authors:

   1. Jee Min Kim
   2. Claudia C Carcamo
   3. Sina Jazani
   4. Zepei Xie
   5. Xinyu A Feng
   6. Maryam Yamadi
   7. Matthew Poyton
   8. Katie L Holland
   9. Jonathan B Grimm
   10. Luke D Lavis
   11. Taekjip Ha
   12. Carl Wu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript describes fundamental single-molecule correlative force and fluorescence microscopy experiments to visualize the 1D diffusion dynamics and long-range nucleosome sliding activity of the yeast chromatin remodelers, RSC and ISW2. Compelling evidence shows that both remodelers exhibit 1D diffusion on bare DNA but utilize different mechanisms, with RSC primarily hopping and ISW2 mainly sliding on DNA. These results will be of interest to researchers working on chromatin remodeling.

   Reviewed by eLife

10. Phosphorylation, disorder, and phase separation govern the behavior of Frequency in the fungal circadian clock

    This article has 7 authors:

    1. Daniyal Tariq
    2. Nicole Maurici
    3. Bradley M Bartholomai
    4. Siddarth Chandrasekaran
    5. Jay C Dunlap
    6. Alaji Bah
    7. Brian R Crane

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This manuscript is a fundamental contribution to the understanding of the role of intrinsically disordered proteins in circadian clocks and the potential involvement of phase separation mechanisms. The authors convincingly report on the structural and biochemical aspects and the molecular interactions of the intrinsically disordered protein FRQ. The paper will be of interest to scientists focusing on circadian clock regulation, liquid-liquid phase separation, and phosphorylation.

    Reviewed by eLife