eLife

Latest preprint reviews

1. Direct modulation of TRPM8 ion channels by rapamycin and analog macrolide immunosuppressants

   This article has 13 authors:

   1. Balázs István Tóth
   2. Bahar Bazeli
   3. Annelies Janssens
   4. Erika Lisztes
   5. Márk Racskó
   6. Balázs Kelemen
   7. Mihály Herczeg
   8. Tamás Milán Nagy
   9. Katalin E Kövér
   10. Argha Mitra
   11. Attila Borics
   12. Tamás Bíró
   13. Thomas Voets

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript presents valuable findings showing that rapamycin directly activates the cool-sensing ion channel, TRPM8, acting through a different binding site than other small-molecule cooling agents such as menthol. The use of Ca2+-imaging, electrophysiology, and computational biology provides solid evidence to support the finding. The authors also present a novel NMR-based method to help identify details of the binding site interactions. In this revised version, some analysis and the presentation have been corrected and improved. Their findings provide insights into TRP channel pharmacology and may indicate previously unknown physiological effects or therapeutic mechanisms of the immunosuppressant, rapamycin.

   Reviewed by eLife

2. The identification of extensive samples of motor units in human muscles reveals diverse effects of neuromodulatory inputs on the rate coding

   This article has 5 authors:

   1. Simon Avrillon
   2. François Hug
   3. Roger M Enoka
   4. Arnault HD Caillet
   5. Dario Farina

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Leveraging state-of-the-art experimental and analytical approaches, this important study characterizes the recruitment and activation of large populations of human motor units during slow isometric contractions in two lower limb muscles. Evidence for the main claims is solid and advances our understanding of how humans generate and control voluntary force.

   Reviewed by eLife

3. Transcriptomic profiling of Schlemm’s canal cells reveals a lymphatic-biased identity and three major cell states

   This article has 15 authors:

   1. Revathi Balasubramanian
   2. Krishnakumar Kizhatil
   3. Taibo Li
   4. Nicholas Tolman
   5. Aakriti Bhandari
   6. Graham Clark
   7. Violet Bupp-Chickering
   8. Ruth A Kelly
   9. Sally Zhou
   10. John Peregrin
   11. Marina Simón
   12. Christa Montgomery
   13. W Daniel Stamer
   14. Jiang Qian
   15. Simon WM John

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable study has characterized the unique expression of Schlemm's canal endothelial cells (SECs) using FACS-sorted specific cell bulk RNA-Seq and scRNA-/snRNA-Seq of mouse SECs. The compelling study identified novel biomarkers for SECs and molecular markers for two inner wall SEC states and outwall SECs in mouse eyes. Significant gene networks and pathways were elucidated for their potential contribution to glaucoma pathogenesis, providing targets for further research in relation to glaucoma.

   Reviewed by eLife

4. Two long-axis dimensions of hippocampal-cortical integration support memory function across the adult lifespan

   This article has 12 authors:

   1. Kristin Nordin
   2. Robin Pedersen
   3. Farshad Falahati
   4. Jarkko Johansson
   5. Filip Grill
   6. Micael Andersson
   7. Saana M Korkki
   8. Lars Bäckman
   9. Andrew Zalesky
   10. Anna Rieckmann
   11. Lars Nyberg
   12. Alireza Salami

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This fundamental work demonstrates the importance of considering overlapping modes of functional organization (i.e. gradients) in the hippocampus, showing associations with aging, dopaminergic receptor distribution and episodic memory. The evidence supporting the conclusions is convincing, although not all analyses were performed in a replication sample. The work will be of broad interest to basic and clinical neuroscientists.

   Reviewed by eLife

5. Genetic stability of Mycobacterium smegmatis under the stress of first-line antitubercular agents

   This article has 6 authors:

   1. Dániel Molnár
   2. Éva Viola Surányi
   3. Tamás Trombitás
   4. Dóra Füzesi
   5. Rita Hirmondó
   6. Judit Toth

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This useful study reports on the impact of antibiotic pressure on the genomic stability of the mc2155 strain of Mycobacterium smegmatis, a model for Mycobacterium tuberculosis. The findings of the study indicate that exposure to antibiotics did not lead to the development of new adaptive mutations in controlled laboratory environments, challenging the notion that antibiotic resistance arises from drug-induced microevolution. The genomic analysis provides detailed insights into the stability of M. smegmatis following exposure to standard TB treatment antibiotics, and the evidence suggesting that antibiotic pressure does not contribute to the emergence of new adaptive mutations is solid.

   Reviewed by eLife

6. High-density sampling reveals volume growth in human tumours

   This article has 6 authors:

   1. Arman Angaji
   2. Michel Owusu
   3. Christoph Velling
   4. Nicola Dick
   5. Donate Weghorn
   6. Johannes Berg

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The article uses a cell-based model to investigate how mutations and cells spread throughout a tumour. The paper uses published data and the proposed model to understand how growth and death mechanisms lead to the observed data. This work provides an important insight into the early stages of tumour development. From the work provided here, the results are convincing, using a thorough analysis.

   Reviewed by eLife

7. Connecting Chromatin Structures to Gene Regulation Using Dynamic Polymer Simulations

   This article has 6 authors:

   1. Yi Fu
   2. Tianxiao Zhao
   3. Finnegan Clark
   4. Sofia Nomikou
   5. Aristotelis Tsirigos
   6. Timothée Lionnet

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents a valuable optimization algorithm to identify polymer models that best fit population-averaged chromosome contact data that will be of interest to physicists and biologists working on chromatin organization. The conclusions are supported by solid evidence.

   Reviewed by eLife

8. Single-nucleus multiomics reveals the gene regulatory networks underlying sex determination of murine primordial germ cells

   This article has 8 authors:

   1. Adriana K Alexander
   2. Karina F Rodriguez
   3. Yu-Ying Chen
   4. Ciro Amato
   5. Martin A Estermann
   6. Barbara Nicol
   7. Xin Xu
   8. Humphrey HC Yao

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study reports single-nucleus multiomics-based profiling of transcriptome and chromatin accessibility of mouse XX and XY primordial germ cells (PGCs). The main conclusions of this study, which will be of interest to developmental and reproductive biologists, as well as andrologists, are supported by convincing data.

   Reviewed by eLife

9. Hosts manipulate lifestyle switch and pathogenicity heterogeneity of opportunistic pathogens in the single-cell resolution

   This article has 14 authors:

   1. Ziguang Wang
   2. Shuai Li
   3. Sheng Zhang
   4. Tianyu Zhang
   5. Yujie Wu
   6. Anqi Liu
   7. Kui Wang
   8. Xiaowen Ji
   9. Haiqun Cao
   10. Yinglao Zhang
   11. Eng King Tan
   12. Yongcheng Wang
   13. Yirong Wang
   14. Wei Liu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The fundamental findings of this work substantially advance our understanding of the impact of the host on its gut microbes. The authors provided compelling evidence at single-cell resolution that the host can drive heterogeneity in the populations of gut microbes with significant consequences for the host physiology.

   Reviewed by eLife

10. Partial rejuvenation of the spermatogonial stem cell niche after gender-affirming hormone therapy in trans women

    This article has 9 authors:

    1. Emily Delgouffe
    2. Samuel Madureira Silva
    3. Frédéric Chalmel
    4. Wilfried Cools
    5. Camille Raets
    6. Kelly Tilleman
    7. Guy T'Sjoen
    8. Yoni Baert
    9. Ellen Goossens

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This important study presents new knowledge of the spermatogonial stem cell (SSC) niche in trans women after gender-affirming hormone therapy (GAHT). The evidence supporting the claims is convincing. The work will be of interest to researchers and clinicians working in the field of reproductive medicine and andrology.

    Reviewed by eLife