eLife

Latest preprint reviews

1. Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays

   This article has 8 authors:

   1. Jonathan G Van Vranken
   2. Jiaming Li
   3. Julian Mintseris
   4. Ting-Yu Wei
   5. Catherine M Sniezek
   6. Meagan Gadzuk-Shea
   7. Steven P Gygi
   8. Devin K Schweppe

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The study provides a valuable showcase of a workflow to perform large-scale characterization of drug mechanisms of action using proteomics in which on-target and off-targets of 166 compounds using proteome solubility analysis in living cells and cell lysates were determined. The evidence supporting the claims of the authors is solid, however, the inclusion of more replicate experiments and more statistical rigor would have strengthened the study. This will be of broad interest to medicinal chemists, toxicologists, computational biologists and biochemists.

   Reviewed by PREreview, eLife

2. Spontaneous activity of striatal projection neurons supports maturation of striatal inputs to substantia nigra dopaminergic neurons

   This article has 8 authors:

   1. Bojana Kokinovic
   2. Patricia Seja
   3. Angelica Donati
   4. Maria Ryazantseva
   5. Alban de Kerchove d’Exaerde
   6. Serge N. Schiffmann
   7. Tomi Taira
   8. Svetlana M. Molchanova

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable study describes early postnatal compartmental differences in the functional maturation of striatal projection neurons. It explores how the postnatal activity of these neurons may determine the GABAergic innervation of dopaminergic neurons in the adult substantia nigra pars compacta. While the functional characterization of striatal neuron development is solid, analysis of how early postnatal activity of striatal projection neurons shapes their functional innervation of dopaminergic neurons is incomplete.

   Reviewed by eLife

3. Role of N343 glycosylation on the SARS-CoV-2 S RBD structure and co-receptor binding across variants of concern

   This article has 7 authors:

   1. Callum M Ives
   2. Linh Nguyen
   3. Carl A Fogarty
   4. Aoife M Harbison
   5. Yves Durocher
   6. John Klassen
   7. Elisa Fadda

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents an important finding on the structural role of glycosylation at position N343 of the SARS-CoV-2 spike protein's receptor-binding domain in maintaining its stability, with implications across different variants of concern. The evidence supporting the claims of the authors is convincing, since appropriate and validated methodology in line with current state-of-the-art has been approached. The work will be of interest to evolutionary virologists.

   Reviewed by eLife

4. Deletion of sulfate transporter SUL1 extends yeast replicative lifespan via reduced PKA signaling instead of decreased sulfate uptake

   This article has 7 authors:

   1. Juan Long
   2. Meng Ma
   3. Yuting Chen
   4. Bo Gong
   5. Yi Zheng
   6. Hao Li
   7. Jing Yang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study offers a valuable contribution to the understanding of how inorganic nutrient transporters, particularly SUL1, influence yeast lifespan through signaling pathways rather than transport functions. The findings suggest a novel link between SUL1 deletion and extended replicative lifespan, supported by transcriptomic and stress-response data. However, the strength of the evidence remains incomplete, with key experiments-such as sulfate supplementation tests, functional autophagy validation, and transport assays-either missing or insufficiently described. As a result, while the manuscript presents promising insights, additional work is needed to robustly support its conclusions.

   Reviewed by eLife

5. The geometric basis of epithelial convergent extension

   This article has 5 authors:

   1. Fridtjof Brauns
   2. Nikolas H Claussen
   3. Matthew F Lefebvre
   4. Eric F Wieschaus
   5. Boris I Shraiman

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study analyzes in an original way how tension pattern dynamics can reveal the contribution of active versus passive intercalation during tissue elongation. The authors develop a compelling, elegant analytical framework (isogonal tension decomposition) to disentangle the passive (adjacent tissues pulling) and active (local tension anisotropy) contributions to intercalation events. This allows the generation of global maps of tissue mechanics that will be extremely helpful in the field of biomechanics.

   Reviewed by eLife

6. S-acylation of NLRP3 provides a nigericin sensitive gating mechanism that controls access to the Golgi

   This article has 2 authors:

   1. Daniel M Williams
   2. Andrew A Peden

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important paper implicates S-acylation of Cys-130 in recruitment of the inflammasome receptor NLRP3 to the Golgi, and it provides convincing evidence that S-acylation plays a key role in response to the stress induced by nigericin treatment. While Cys-130 does seem to play a previously unappreciated role in membrane association of NLRP3, further work will be needed to clarify the details of the mechanism.

   Reviewed by eLife

7. Mitosis sets nuclear homeostasis of cancer cells under confinement

   This article has 6 authors:

   1. Malèke Mouelhi
   2. Alexis Saffon
   3. Morgane Roinard
   4. Hélène Delanoë-Ayari
   5. Sylvain Monnier
   6. Charlotte Rivière

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study describes the new observation that nuclear volume responds to confinement in a manner that requires transit through mitosis. The authors present solid evidence demonstrating that nuclear volume decreases upon nuclear envelope reformation under confinement in a manner that reestablishes a homeostatic state of nuclear envelope tension. Additional experimental support could provide a more complete case for the proposed underlying mechanisms governing this response. The work will be of broad interest to cell biologists and those interested in cell and organismal scaling.

   Reviewed by eLife

8. The relationship between gut and nasopharyngeal microbiome composition can predict the severity of COVID-19

   This article has 26 authors:

   1. Benita Martin-Castaño
   2. Patricia Diez-Echave
   3. Jorge García-García
   4. Laura Hidalgo-García
   5. Antonio Jesús Ruiz-Malagon
   6. José Alberto Molina-Tijeras
   7. María Jesús Rodríguez-Sojo
   8. Anaïs Redruello-Romero
   9. Margarita Martínez-Zaldívar
   10. Emilio Mota
   11. Fernando Cobo
   12. Xando Díaz-Villamarin
   13. Marta Alvarez-Estevez
   14. Federico García
   15. Concepción Morales-García
   16. Silvia Merlos
   17. Paula Garcia-Flores
   18. Manuel Colmenero-Ruiz
   19. José Hernández-Quero
   20. Maria Nuñez
   21. Maria Elena Rodriguez-Cabezas
   22. Angel Carazo
   23. Javier Martin
   24. Rocio Moron
   25. Alba Rodríguez Nogales
   26. Julio Galvez

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This potentially valuable work characterizes the changes in the microbial composition of the nasal and fecal microbiomes in COVID-19 patients based on disease severity. This study enhances the understanding of COVID-19 severity predictors by identifying changes in bacterial species abundance in nasopharyngeal and fecal samples as a biomarker for predicting disease severity. The methods and statistics used appear to be solid and in line with the standards of the field.

   Reviewed by eLife

9. Sibling chimerism among microglia in marmosets

   This article has 11 authors:

   1. Ricardo C.H. del Rosario
   2. Fenna M. Krienen
   3. Qiangge Zhang
   4. Melissa Goldman
   5. Curtis Mello
   6. Alyssa Lutservitz
   7. Kiku Ichihara
   8. Alec Wysoker
   9. James Nemesh
   10. Guoping Feng
   11. Steven A. McCarroll

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental study substantially advances our understanding of sibling chimerism in marmosets by demonstrating that chimerism is limited to hematopoietic cells. The evidence supporting these findings is compelling, demonstrated through comprehensive analyses, including single-cell RNA-seq data from multiple individuals and tissues. The work will be of broad interest to many fields of biology.

   Reviewed by eLife

10. Boosting biodiversity monitoring using smartphone-driven, rapidly accumulating community-sourced data

    This article has 6 authors:

    1. Keisuke Atsumi
    2. Yuusuke Nishida
    3. Masayuki Ushio
    4. Hirotaka Nishi
    5. Takanori Genroku
    6. Shogoro Fujiki

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This important study presents findings of great practical value, offering fresh insights into natural species distributions across Japan. By combining multiple data sources (including those from non-academic sectors, aka citizen scientists), the manuscript also presents a compelling new tool that can be used to aid conservation agendas, detect species distribution changes, and testing of ecological theories.

    Reviewed by eLife