eLife

Latest preprint reviews

1. Structural characterization and dynamics of AdhE ultrastructures from Clostridium thermocellum show a containment strategy for toxic intermediates

   This article has 9 authors:

   1. Samantha J Ziegler
   2. Brandon C Knott
   3. Josephine N Gruber
   4. Neal N Hengge
   5. Qi Xu
   6. Daniel G Olson
   7. Eduardo E Romero
   8. Lydia-Marie Joubert
   9. Yannick J Bomble

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This work presents valuable information on the structure of the spirosome's native extended conformation as the active form of the aldehyde-alcohol dehydrogenase (AdhE) enzyme. The evidence is solid, although the work does not provide a mechanistic understanding of the function and dynamics of AdhE.

   Reviewed by eLife

2. Amoeboid cells undergo durotaxis with soft end polarized NMIIA

   This article has 9 authors:

   1. Chenlu Kang
   2. Pengcheng Chen
   3. Xin Yi
   4. Dong Li
   5. Yiping Hu
   6. Yihong Yang
   7. Huaqing Cai
   8. Bo Li
   9. Congying Wu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents an important finding on durotaxis in various amoeboid cells that is independent of focal adhesions. The evidence supporting the authors' claims is compelling. The work will be of interest to cell biologists and biophysicists working on rigidity sensing, the cytoskeleton, and cell migration.

   Reviewed by eLife

3. Heterogeneity of Sonic Hedgehog response dynamics and fate specification in single neural progenitors

   This article has 6 authors:

   1. Fengzhu Xiong
   2. Andrea R. Tentner
   3. Sandy Nandagopal
   4. Tom W. Hiscock
   5. Peng Huang
   6. Sean G. Megason

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents an important study of the relationship between morphogen signaling and cell fate choices in the forming zebrafish neural tube, addressing a topical question in developmental biology. The authors provide a solid characterization of the precision limit for gene regulatory networks interpreting Shh, with single-cell resolution and state-of-the-art in vivo approaches. However, the analyses are at times incomplete and would benefit from a higher number of cell traces. With the analyses strengthened, this work will be of interest to developmental biologists interested in cellular decision-making.

   Reviewed by eLife

4. Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross-stage recognition of glutamate-rich repeats

   This article has 17 authors:

   1. Axelle Amen
   2. Randy Yoo
   3. Amanda Fabra-García
   4. Judith Bolscher
   5. William JR Stone
   6. Isabelle Bally
   7. Sebastián Dergan-Dylon
   8. Iga Kucharska
   9. Roos M de Jong
   10. Marloes de Bruijni
   11. Teun Bousema
   12. C Richter King
   13. Randall S MacGill
   14. Robert W Sauerwein
   15. Jean-Philippe Julien
   16. Pascal Poignard
   17. Matthijs M Jore

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study reports important results and new insights into humoral immune responses to Plasmodium falciparum sexual stage proteins. The experiments are based on the use of target-agnostic memory B cell sorting and screening approaches as well as several state-of-the-art technologies. The authors present compelling evidence that one antibody, B1E11K, is cross-reactive with multiple proteins containing glutamate-rich repeats through homotypic interactions, a process similar to what has been observed for Plasmodium circumsporozoite protein repeat-directed antibodies.

   Reviewed by eLife

5. Value construction through sequential sampling explains serial dependencies in decision making

   This article has 4 authors:

   1. Ariel Zylberberg
   2. Akram Bakkour
   3. Daphna Shohamy
   4. Michael N Shadlen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study addresses key assumptions underlying current models of the formation of value-based decisions. The authors provide convincing evidence that the subjective values human participants assign to items change across sequences of multiple decisions. They establish methods to detect these changes in frequently used behavioral task designs.

   Reviewed by eLife

6. Acquisition phase-specific contribution of climbing fiber transmission to cerebellum-dependent motor memory in mice

   This article has 6 authors:

   1. Jewoo Seo
   2. Seung Ha Kim
   3. Jaegeon Lee
   4. Min Seok Kim
   5. Yong-Seok Lee
   6. Sang Jeong Kim

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents potentially valuable insights into the role of climbing fibers in cerebellar learning. The main claim is that climbing fiber activity is necessary for optokinetic reflex adaptation, but is dispensable for its long-term consolidation. There is evidence to support the first part of this claim, though it requires a clearer demonstration of the penetrance and selectivity of the manipulation. However, support for the latter part of the claim is incomplete owing to methodological concerns, including the robustness of the CF marking and manipulation approach and the unclear efficacy of longer-duration climbing fiber activity suppression.

   Reviewed by eLife

7. A Connectome of the Male Drosophila Ventral Nerve Cord

   This article has 84 authors:

   1. Shin-ya Takemura
   2. Kenneth J Hayworth
   3. Gary B Huang
   4. Michal Januszewski
   5. Zhiyuan Lu
   6. Elizabeth C Marin
   7. Stephan Preibisch
   8. C Shan Xu
   9. John Bogovic
   10. Andrew S Champion
   11. Han SJ Cheong
   12. Marta Costa
   13. Katharina Eichler
   14. William Katz
   15. Christopher Knecht
   16. Feng Li
   17. Billy J Morris
   18. Christopher Ordish
   19. Patricia K Rivlin
   20. Philipp Schlegel
   21. Kazunori Shinomiya
   22. Tomke Stürner
   23. Ting Zhao
   24. Griffin Badalamente
   25. Dennis Bailey
   26. Paul Brooks
   27. Brandon S Canino
   28. Jody Clements
   29. Michael Cook
   30. Octave Duclos
   31. Christopher R Dunne
   32. Kelli Fairbanks
   33. Siqi Fang
   34. Samantha Finley-May
   35. Audrey Francis
   36. Reed George
   37. Marina Gkantia
   38. Kyle Harrington
   39. Gary Patrick Hopkins
   40. Joseph Hsu
   41. Philip M Hubbard
   42. Alexandre Javier
   43. Dagmar Kainmueller
   44. Wyatt Korff
   45. Julie Kovalyak
   46. Dominik Krzemiński
   47. Shirley A Lauchie
   48. Alanna Lohff
   49. Charli Maldonado
   50. Emily A Manley
   51. Caroline Mooney
   52. Erika Neace
   53. Matthew Nichols
   54. Omotara Ogundeyi
   55. Nneoma Okeoma
   56. Tyler Paterson
   57. Elliott Phillips
   58. Emily M Phillips
   59. Caitlin Ribeiro
   60. Sean M Ryan
   61. Jon Thomson Rymer
   62. Anne K Scott
   63. Ashley L Scott
   64. David Shepherd
   65. Aya Shinomiya
   66. Claire Smith
   67. Natalie Smith
   68. Alia Suleiman
   69. Satoko Takemura
   70. Iris Talebi
   71. Imaan FM Tamimi
   72. Eric T Trautman
   73. Lowell Umayam
   74. John J Walsh
   75. Tansy Yang
   76. Gerald M Rubin
   77. Louis K Scheffer
   78. Jan Funke
   79. Stephan Saalfeld
   80. Harald F Hess
   81. Stephen M Plaza
   82. Gwyneth M Card
   83. Gregory SXE Jefferis
   84. Stuart Berg

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This landmark paper introduces the generation and analysis of a connectome resource of the entire ventral nerve cord of a fruit fly which is one of the top model organisms to investigate how a nervous system forms and functions. The work introduces new and improved approaches - from tissue preparation to automated reconstruction - to generate a detailed connectome from a complex adult ventral nerve cord. This extensive new dataset provides cell type and lineage annotations, putative neurotransmitter expression information, and the potential to link to genetic driver lines, with compelling evidence to support the claims made.

   Reviewed by eLife

8. The zinc transporter Slc30a1 (ZnT1) in macrophages plays a protective role against attenuated Salmonella

   This article has 6 authors:

   1. Pinanong Na-Phatthalung
   2. Shumin Sun
   3. Enjun Xie
   4. Jia Wang
   5. Junxia Min
   6. Fudi Wang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Work described in this manuscript reveals the importance of the zinc transporter SLC30A1 in the antimicrobial function of macrophages, specifically against Salmonella. Cell-targeted deletion of the zinc transporter increased susceptibility of mice to systemic infection with Salmonella, leading to decreases in several cell functions such as nos2 expression. The authors argue that zinc homeostasis promotes macrophage cell function that is not conductive to the intracellular proliferation of Salmonella. This study provides novel and supportive evidence for a new pathway in nutritional immunity.

   Reviewed by eLife

9. Insights into early animal evolution from the genome of the xenacoelomorph worm Xenoturbella bocki

   This article has 20 authors:

   1. Philipp H Schiffer
   2. Paschalis Natsidis
   3. Daniel J Leite
   4. Helen E Robertson
   5. François Lapraz
   6. Ferdinand Marlétaz
   7. Bastian Fromm
   8. Liam Baudry
   9. Fraser Simpson
   10. Eirik Høye
   11. Anne C Zakrzewski
   12. Paschalia Kapli
   13. Katharina J Hoff
   14. Steven Müller
   15. Martial Marbouty
   16. Heather Marlow
   17. Richard R Copley
   18. Romain Koszul
   19. Peter Sarkies
   20. Maximilian J Telford

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors provide a high quality genome of the xenacoelomorph worm Xenoturbella bocki and discuss its structure and evolution. Understanding the genomic structure of this group provides important insights into bilaterian evolution. The authors make a solid case that the data they present can support the placement of Xenacoelomorpha within the deuterostomes rather than as a sister group to all other bilaterians, but do not unequivocally reject the competing scenario.

   Reviewed by eLife

10. Deep Mutagenesis of a Transporter for Uptake of a Non-Native Substrate Identifies Conformationally Dynamic Regions

    This article has 9 authors:

    1. Heather J Ellis
    2. Matthew Chan
    3. Balaji Selvam
    4. Evan Walter
    5. Christine A Devlin
    6. Steven K Szymanski
    7. Loren Keith Henry
    8. Diwakar Shukla
    9. Erik Procko

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      Serotonin is an important neurotransmitter and its synaptic concentration is controlled by re-uptake by the sodium-coupled serotonin transporter SERT. The manuscript by Chan et al reports results from a systematic deep mutagenesis approach to study the surface expression and APP+ (5HT analogue) transport mechanism of the human serotonin transporter. The authors complement this experimental evidence with large-scale molecular simulations of the transporter in the presence of APP+. The use of deep mutagenesis and large-scale adaptive sampling simulations is impressive, and could contribute to understanding the structural requirements for folding and how transporters evolve to recognize different substrates.

    Reviewed by eLife